Antimicrobial compound from Trichoderma harzianum, an endophytic fungus associated with ginger (Zingiber officinale)
Background: Genus Trichoderma of fungal kingdom are largely used as biological control agents due to broad-spectrum activity against plant pathogens. Objective: This study aimed to investigate Trichoderma harzianum, an endophytic fungus obtained from ginger (Zingiber officinale) leaves. Methods: The...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Medicine
2021-08-01
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| Series: | JKKI (Jurnal Kedokteran dan Kesehatan Indonesia) |
| Subjects: | |
| Online Access: | https://journal.uii.ac.id/JKKI/article/view/18839 |
| _version_ | 1827906243818160128 |
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| author | Harwoko Harwoko Jungho Lee Georgios Daletos Michael Feldbrügge Rainer Kalscheuer Peter Proksch |
| author_facet | Harwoko Harwoko Jungho Lee Georgios Daletos Michael Feldbrügge Rainer Kalscheuer Peter Proksch |
| author_sort | Harwoko Harwoko |
| collection | DOAJ |
| description | Background: Genus Trichoderma of fungal kingdom are largely used as biological control agents due to broad-spectrum activity against plant pathogens.
Objective: This study aimed to investigate Trichoderma harzianum, an endophytic fungus obtained from ginger (Zingiber officinale) leaves.
Methods: The chemical structures of the isolated compounds were deduced on the basis of UV, 1H NMR and MS data analyses, as well as comparison with literature.
Results: Two known tetramic acid derivatives were isolated from this fungus, including harzianic acid (A) and isoharzianic acid (B). Compound B inhibited the growth of a corn pathogenic fungus, Ustilago maydis, with inhibition zone diameter (39 ± 0.33 mm) larger than nystatin (29 mm). Additionally, iso-HA (B) revealed antibacterial effect towards Staphylococcus aureus with MIC value of 25 µM. However, both compounds showed no cytotoxicity against human cervical and ovarian cancer cell lines.
Conclusion: T. harzianum produced antimicrobial compound like iso-HA which has potential application either in agricultural or health. |
| first_indexed | 2024-03-13T00:53:47Z |
| format | Article |
| id | doaj.art-0034423bf84c4979b5ca91e2db526b87 |
| institution | Directory Open Access Journal |
| issn | 2085-4145 2527-2950 |
| language | English |
| last_indexed | 2024-03-13T00:53:47Z |
| publishDate | 2021-08-01 |
| publisher | Faculty of Medicine |
| record_format | Article |
| series | JKKI (Jurnal Kedokteran dan Kesehatan Indonesia) |
| spelling | doaj.art-0034423bf84c4979b5ca91e2db526b872023-07-07T07:41:35ZengFaculty of MedicineJKKI (Jurnal Kedokteran dan Kesehatan Indonesia)2085-41452527-29502021-08-0115115710.20885/JKKI.Vol12.Iss2.art814941Antimicrobial compound from Trichoderma harzianum, an endophytic fungus associated with ginger (Zingiber officinale)Harwoko Harwoko0https://orcid.org/0000-0002-4111-7147Jungho Lee1Georgios Daletos2Michael Feldbrügge3Rainer Kalscheuer4Peter Proksch5Department of Pharmacy, Faculty of Health Sciences, Universitas Jenderal Soedirman, Purwokerto, IndonesiaInstitute for Microbiology, Heinrich-Heine-Universitaet, Universitaetsstrasse 1, DüsseldorfDepartment of Chemical Engineering, Massachusetts Institute of Technology, MassachusettsInstitute for Microbiology, Heinrich-Heine-Universitaet, Universitaetsstrasse 1, DüsseldorfInstitute of Pharmaceutical Biology and Biotechnology, Heinrich-Heine-Universitaet, Universitaetsstrasse 1, DüsseldorfInstitute of Pharmaceutical Biology and Biotechnology, Heinrich-Heine-Universitaet, Universitaetsstrasse 1, DüsseldorfBackground: Genus Trichoderma of fungal kingdom are largely used as biological control agents due to broad-spectrum activity against plant pathogens. Objective: This study aimed to investigate Trichoderma harzianum, an endophytic fungus obtained from ginger (Zingiber officinale) leaves. Methods: The chemical structures of the isolated compounds were deduced on the basis of UV, 1H NMR and MS data analyses, as well as comparison with literature. Results: Two known tetramic acid derivatives were isolated from this fungus, including harzianic acid (A) and isoharzianic acid (B). Compound B inhibited the growth of a corn pathogenic fungus, Ustilago maydis, with inhibition zone diameter (39 ± 0.33 mm) larger than nystatin (29 mm). Additionally, iso-HA (B) revealed antibacterial effect towards Staphylococcus aureus with MIC value of 25 µM. However, both compounds showed no cytotoxicity against human cervical and ovarian cancer cell lines. Conclusion: T. harzianum produced antimicrobial compound like iso-HA which has potential application either in agricultural or health.https://journal.uii.ac.id/JKKI/article/view/18839trichoderma harzianumendophytic fungusantimicrobialisoharzianic acid |
| spellingShingle | Harwoko Harwoko Jungho Lee Georgios Daletos Michael Feldbrügge Rainer Kalscheuer Peter Proksch Antimicrobial compound from Trichoderma harzianum, an endophytic fungus associated with ginger (Zingiber officinale) JKKI (Jurnal Kedokteran dan Kesehatan Indonesia) trichoderma harzianum endophytic fungus antimicrobial isoharzianic acid |
| title | Antimicrobial compound from Trichoderma harzianum, an endophytic fungus associated with ginger (Zingiber officinale) |
| title_full | Antimicrobial compound from Trichoderma harzianum, an endophytic fungus associated with ginger (Zingiber officinale) |
| title_fullStr | Antimicrobial compound from Trichoderma harzianum, an endophytic fungus associated with ginger (Zingiber officinale) |
| title_full_unstemmed | Antimicrobial compound from Trichoderma harzianum, an endophytic fungus associated with ginger (Zingiber officinale) |
| title_short | Antimicrobial compound from Trichoderma harzianum, an endophytic fungus associated with ginger (Zingiber officinale) |
| title_sort | antimicrobial compound from trichoderma harzianum an endophytic fungus associated with ginger zingiber officinale |
| topic | trichoderma harzianum endophytic fungus antimicrobial isoharzianic acid |
| url | https://journal.uii.ac.id/JKKI/article/view/18839 |
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