The DcPS1 cooperates with OSDLa during pollen development and 2n gamete production in carnation meiosis
Abstract Background Deciphering the mechanisms of meiosis has important implications for potential applications in plant breeding programmes and species evolution. However, the process of meiosis is poorly understood in carnation, which is famous for its cut flowers. Results We report that Dianthus...
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| Format: | Article |
| Language: | English |
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BMC
2022-05-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-022-03648-z |
| _version_ | 1818213870722875392 |
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| author | Xuhong Zhou Shuying Li Xiaomi Yang |
| author_facet | Xuhong Zhou Shuying Li Xiaomi Yang |
| author_sort | Xuhong Zhou |
| collection | DOAJ |
| description | Abstract Background Deciphering the mechanisms of meiosis has important implications for potential applications in plant breeding programmes and species evolution. However, the process of meiosis is poorly understood in carnation, which is famous for its cut flowers. Results We report that Dianthus caryophyllus parallel spindle 1 (DcPS1) regulates omission of second division like a (OSDLa) during pollen development and 2n gamete production in carnation meiosis. In DcPS1 and OSDLa RNAi lines, an absence of the second meiotic division and the abnormal orientation of spindles at meiosis II might be the main reason for dyad/triad formation, resulting in unreduced gametes. We also found that carnation OSDLa interacted with DcPS1 and DcRAD51D. In the DcPS1 RNAi lines, a decrease in OSDLa and DcRAD51D expression was observed. In the OSDLa RNAi lines, a decrease in DcPS1 and DcRAD51D expression was also observed. We propose that DcPS1 regulates OSDLa expression, allowing entry into meiosis II and the proper orientation of the metaphase II spindle in meiosis II. We also propose that OSDLa regulates DcRAD51D expression, allowing for homologous recombination. Conclusions These results suggest a critical role for DcPS1 and OSDLa in diplogamete production during meiosis and open a new pathway for meiosis-related studies. |
| first_indexed | 2024-12-12T06:11:10Z |
| format | Article |
| id | doaj.art-003636510f814a32ad2420338b76d2c9 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-12T06:11:10Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-003636510f814a32ad2420338b76d2c92022-12-22T00:35:10ZengBMCBMC Plant Biology1471-22292022-05-0122111110.1186/s12870-022-03648-zThe DcPS1 cooperates with OSDLa during pollen development and 2n gamete production in carnation meiosisXuhong Zhou0Shuying Li1Xiaomi Yang2Office of Science and Technology, Yunnan University of Chinese MedicineFlower Research Institute, Yunnan Academy of Agricultural Sciences, National Engineering Research Center for Ornamental HorticultureOffice of Science and Technology, Yunnan University of Chinese MedicineAbstract Background Deciphering the mechanisms of meiosis has important implications for potential applications in plant breeding programmes and species evolution. However, the process of meiosis is poorly understood in carnation, which is famous for its cut flowers. Results We report that Dianthus caryophyllus parallel spindle 1 (DcPS1) regulates omission of second division like a (OSDLa) during pollen development and 2n gamete production in carnation meiosis. In DcPS1 and OSDLa RNAi lines, an absence of the second meiotic division and the abnormal orientation of spindles at meiosis II might be the main reason for dyad/triad formation, resulting in unreduced gametes. We also found that carnation OSDLa interacted with DcPS1 and DcRAD51D. In the DcPS1 RNAi lines, a decrease in OSDLa and DcRAD51D expression was observed. In the OSDLa RNAi lines, a decrease in DcPS1 and DcRAD51D expression was also observed. We propose that DcPS1 regulates OSDLa expression, allowing entry into meiosis II and the proper orientation of the metaphase II spindle in meiosis II. We also propose that OSDLa regulates DcRAD51D expression, allowing for homologous recombination. Conclusions These results suggest a critical role for DcPS1 and OSDLa in diplogamete production during meiosis and open a new pathway for meiosis-related studies.https://doi.org/10.1186/s12870-022-03648-zMeiosis2n gameteSpindleDcPS1OSDLa |
| spellingShingle | Xuhong Zhou Shuying Li Xiaomi Yang The DcPS1 cooperates with OSDLa during pollen development and 2n gamete production in carnation meiosis BMC Plant Biology Meiosis 2n gamete Spindle DcPS1 OSDLa |
| title | The DcPS1 cooperates with OSDLa during pollen development and 2n gamete production in carnation meiosis |
| title_full | The DcPS1 cooperates with OSDLa during pollen development and 2n gamete production in carnation meiosis |
| title_fullStr | The DcPS1 cooperates with OSDLa during pollen development and 2n gamete production in carnation meiosis |
| title_full_unstemmed | The DcPS1 cooperates with OSDLa during pollen development and 2n gamete production in carnation meiosis |
| title_short | The DcPS1 cooperates with OSDLa during pollen development and 2n gamete production in carnation meiosis |
| title_sort | dcps1 cooperates with osdla during pollen development and 2n gamete production in carnation meiosis |
| topic | Meiosis 2n gamete Spindle DcPS1 OSDLa |
| url | https://doi.org/10.1186/s12870-022-03648-z |
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