Functional ProTracer identifies patterns of cell proliferation in tissues and underlying regulatory mechanisms
Abstract A genetic system, ProTracer, has been recently developed to record cell proliferation in vivo. However, the ProTracer is initiated by an infrequently used recombinase Dre, which limits its broad application for functional studies employing floxed gene alleles. Here we generated Cre-activate...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-08-01
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| Series: | npj Regenerative Medicine |
| Online Access: | https://doi.org/10.1038/s41536-023-00318-y |
| _version_ | 1797577549390282752 |
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| author | Xiuxiu Liu Maoying Han Wendong Weng Yan Li Wenjuan Pu Kuo Liu Xufeng Li Lingjuan He Ruilin Sun Ruling Shen Yulong He Dandan Liang Yi-Han Chen Qing-Dong Wang Jan S. Tchorz Bin Zhou |
| author_facet | Xiuxiu Liu Maoying Han Wendong Weng Yan Li Wenjuan Pu Kuo Liu Xufeng Li Lingjuan He Ruilin Sun Ruling Shen Yulong He Dandan Liang Yi-Han Chen Qing-Dong Wang Jan S. Tchorz Bin Zhou |
| author_sort | Xiuxiu Liu |
| collection | DOAJ |
| description | Abstract A genetic system, ProTracer, has been recently developed to record cell proliferation in vivo. However, the ProTracer is initiated by an infrequently used recombinase Dre, which limits its broad application for functional studies employing floxed gene alleles. Here we generated Cre-activated functional ProTracer (fProTracer) mice, which enable simultaneous recording of cell proliferation and tissue-specific gene deletion, facilitating broad functional analysis of cell proliferation by any Cre driver. |
| first_indexed | 2024-03-10T22:09:55Z |
| format | Article |
| id | doaj.art-003857d550184788a930ee3f005fbb6c |
| institution | Directory Open Access Journal |
| issn | 2057-3995 |
| language | English |
| last_indexed | 2024-03-10T22:09:55Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Regenerative Medicine |
| spelling | doaj.art-003857d550184788a930ee3f005fbb6c2023-11-19T12:37:03ZengNature Portfolionpj Regenerative Medicine2057-39952023-08-01811710.1038/s41536-023-00318-yFunctional ProTracer identifies patterns of cell proliferation in tissues and underlying regulatory mechanismsXiuxiu Liu0Maoying Han1Wendong Weng2Yan Li3Wenjuan Pu4Kuo Liu5Xufeng Li6Lingjuan He7Ruilin Sun8Ruling Shen9Yulong He10Dandan Liang11Yi-Han Chen12Qing-Dong Wang13Jan S. Tchorz14Bin Zhou15New Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of SciencesNew Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of SciencesNew Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of SciencesNew Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of SciencesNew Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of SciencesKey Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of SciencesKey Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of SciencesWestlake University School of Life SciencesShanghai Model Organisms Center, Inc.Shanghai Laboratory Animal Research CenterCyrus Tang Hematology Center, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Cam-Su Genomic Resources Center, Soochow UniversityState Key Laboratory of Cardiology, Shanghai East Hospital, Tongji University School of MedicineState Key Laboratory of Cardiology, Shanghai East Hospital, Tongji University School of MedicineBioscience Cardiovascular, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D, AstraZenecaNovartis Institutes for BioMedical Research, Novartis Pharma AGNew Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of SciencesAbstract A genetic system, ProTracer, has been recently developed to record cell proliferation in vivo. However, the ProTracer is initiated by an infrequently used recombinase Dre, which limits its broad application for functional studies employing floxed gene alleles. Here we generated Cre-activated functional ProTracer (fProTracer) mice, which enable simultaneous recording of cell proliferation and tissue-specific gene deletion, facilitating broad functional analysis of cell proliferation by any Cre driver.https://doi.org/10.1038/s41536-023-00318-y |
| spellingShingle | Xiuxiu Liu Maoying Han Wendong Weng Yan Li Wenjuan Pu Kuo Liu Xufeng Li Lingjuan He Ruilin Sun Ruling Shen Yulong He Dandan Liang Yi-Han Chen Qing-Dong Wang Jan S. Tchorz Bin Zhou Functional ProTracer identifies patterns of cell proliferation in tissues and underlying regulatory mechanisms npj Regenerative Medicine |
| title | Functional ProTracer identifies patterns of cell proliferation in tissues and underlying regulatory mechanisms |
| title_full | Functional ProTracer identifies patterns of cell proliferation in tissues and underlying regulatory mechanisms |
| title_fullStr | Functional ProTracer identifies patterns of cell proliferation in tissues and underlying regulatory mechanisms |
| title_full_unstemmed | Functional ProTracer identifies patterns of cell proliferation in tissues and underlying regulatory mechanisms |
| title_short | Functional ProTracer identifies patterns of cell proliferation in tissues and underlying regulatory mechanisms |
| title_sort | functional protracer identifies patterns of cell proliferation in tissues and underlying regulatory mechanisms |
| url | https://doi.org/10.1038/s41536-023-00318-y |
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