Integrative Analysis Revealed Stemness Features and a Novel Stemness-Related Classification in Colorectal Cancer Patients
Cancer stem cells play crucial roles in colorectal cancer (CRC) tumorigenesis and treatment response. This study aimed to determine the value of the mRNA stemness index (mRNAsi) in CRC and introduce a stemness-related classification to predict the outcome of patients. mRNAsi scores and RNA sequence...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2022.817509/full |
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| author | Meng-Ling Ye Si-Qi Li Yi-Xin Yin Ke-Zhi Li Ji-Lin Li Bang-Li Hu |
| author_facet | Meng-Ling Ye Si-Qi Li Yi-Xin Yin Ke-Zhi Li Ji-Lin Li Bang-Li Hu |
| author_sort | Meng-Ling Ye |
| collection | DOAJ |
| description | Cancer stem cells play crucial roles in colorectal cancer (CRC) tumorigenesis and treatment response. This study aimed to determine the value of the mRNA stemness index (mRNAsi) in CRC and introduce a stemness-related classification to predict the outcome of patients. mRNAsi scores and RNA sequence data of CRC patients were analyzed. We found that high mRNAsi scores were related to early-stage CRC and a better patient prognosis. Two stemness-based subtypes (subtype I and II) were identified. Patients in subtype I presented a significantly better prognosis than those in subtype II. Patients in these two subtype groups presented significantly different tumor immunity scores and immune cell infiltration patterns. Genomic variations revealed that patients in subtype I had a lower tumor mutation burden than those in subtype II. A three-gene stemness subtype predictor was established, showing good diagnostic value in discriminating patients in different subtypes. A prognostic signature based on five stemness-related genes was established and validated in two independent cohorts and clinical samples, showing a better predictive performance than other clinical parameters. We concluded that mRNAsi scores were associated with the clinical outcome in CRC patients. The stemness-related classification was a promising prognostic predictor for CRC patients. |
| first_indexed | 2024-04-13T17:35:39Z |
| format | Article |
| id | doaj.art-003880e232d447daba58991d73d3a4cf |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-13T17:35:39Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-003880e232d447daba58991d73d3a4cf2022-12-22T02:37:22ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-06-011010.3389/fcell.2022.817509817509Integrative Analysis Revealed Stemness Features and a Novel Stemness-Related Classification in Colorectal Cancer PatientsMeng-Ling YeSi-Qi LiYi-Xin YinKe-Zhi LiJi-Lin LiBang-Li HuCancer stem cells play crucial roles in colorectal cancer (CRC) tumorigenesis and treatment response. This study aimed to determine the value of the mRNA stemness index (mRNAsi) in CRC and introduce a stemness-related classification to predict the outcome of patients. mRNAsi scores and RNA sequence data of CRC patients were analyzed. We found that high mRNAsi scores were related to early-stage CRC and a better patient prognosis. Two stemness-based subtypes (subtype I and II) were identified. Patients in subtype I presented a significantly better prognosis than those in subtype II. Patients in these two subtype groups presented significantly different tumor immunity scores and immune cell infiltration patterns. Genomic variations revealed that patients in subtype I had a lower tumor mutation burden than those in subtype II. A three-gene stemness subtype predictor was established, showing good diagnostic value in discriminating patients in different subtypes. A prognostic signature based on five stemness-related genes was established and validated in two independent cohorts and clinical samples, showing a better predictive performance than other clinical parameters. We concluded that mRNAsi scores were associated with the clinical outcome in CRC patients. The stemness-related classification was a promising prognostic predictor for CRC patients.https://www.frontiersin.org/articles/10.3389/fcell.2022.817509/fullcolorectal cancercancer stemnesstumor microenvironmentimmunotherapyprognosis |
| spellingShingle | Meng-Ling Ye Si-Qi Li Yi-Xin Yin Ke-Zhi Li Ji-Lin Li Bang-Li Hu Integrative Analysis Revealed Stemness Features and a Novel Stemness-Related Classification in Colorectal Cancer Patients Frontiers in Cell and Developmental Biology colorectal cancer cancer stemness tumor microenvironment immunotherapy prognosis |
| title | Integrative Analysis Revealed Stemness Features and a Novel Stemness-Related Classification in Colorectal Cancer Patients |
| title_full | Integrative Analysis Revealed Stemness Features and a Novel Stemness-Related Classification in Colorectal Cancer Patients |
| title_fullStr | Integrative Analysis Revealed Stemness Features and a Novel Stemness-Related Classification in Colorectal Cancer Patients |
| title_full_unstemmed | Integrative Analysis Revealed Stemness Features and a Novel Stemness-Related Classification in Colorectal Cancer Patients |
| title_short | Integrative Analysis Revealed Stemness Features and a Novel Stemness-Related Classification in Colorectal Cancer Patients |
| title_sort | integrative analysis revealed stemness features and a novel stemness related classification in colorectal cancer patients |
| topic | colorectal cancer cancer stemness tumor microenvironment immunotherapy prognosis |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2022.817509/full |
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