Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital
The aim of this study was to determine the prevalence of plasmid-mediated colistin resistance <i>mcr</i>-1 to <i>mcr</i>-5 genes among colistin and multi-drug-resistant Gram-negative bacilli strains isolated from patients in a tertiary hospital in Toluca, Mexico. The presence...
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MDPI AG
2023-08-01
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| Online Access: | https://www.mdpi.com/2076-2607/11/8/1996 |
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| author | Mario Galindo-Méndez Humberto Navarrete-Salazar Reinaldo Pacheco-Vásquez Devanhí Quintas-de la Paz Isabel Baltazar-Jiménez José David Santiago-Luna Laura Guadarrama-Monroy |
| author_facet | Mario Galindo-Méndez Humberto Navarrete-Salazar Reinaldo Pacheco-Vásquez Devanhí Quintas-de la Paz Isabel Baltazar-Jiménez José David Santiago-Luna Laura Guadarrama-Monroy |
| author_sort | Mario Galindo-Méndez |
| collection | DOAJ |
| description | The aim of this study was to determine the prevalence of plasmid-mediated colistin resistance <i>mcr</i>-1 to <i>mcr</i>-5 genes among colistin and multi-drug-resistant Gram-negative bacilli strains isolated from patients in a tertiary hospital in Toluca, Mexico. The presence of <i>mcr</i> genes among the 241 strains collected was assessed by PCR. In the case of <i>mcr</i>-carrying <i>E. coli</i>, further PCR tests were performed to determine the presence of <i>bla</i><sub>CTX-M</sub> and whether the strains belonged to the O25b-ST131 clone. Conjugation experiments were also carried out to assess the horizontal transmission of colistin resistance. A total of twelve strains (5.0%), of which four were <i>E. coli</i>; four were <i>P. aeruginosa</i>; three were <i>K. pneumoniae,</i> and one <i>E. cloacae</i>, were found to be resistant to colistin. Of these strains, two <i>E. coli</i> isolates were found to carry <i>mcr</i>-1, and Southern blot hybridization demonstrated its presence on an approximately 60 kb plasmid. Both <i>mcr</i>-1-carrying <i>E. coli</i> strains were found to co-express <i>bla</i><sub>CTX-M</sub>, belong to the O25b-ST131 clone, and horizontally transmit their colistin resistance. The results of this study confirm the presence of plasmid-mediated colistin resistance in hospitalized patients in Mexico and demonstrated that the multi-drug-resistant O25b-ST131 <i>E. coli</i> clone can acquire <i>mcr</i> genes and transmit such resistance traits to other bacteria. |
| first_indexed | 2024-03-10T23:42:08Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T23:42:08Z |
| publishDate | 2023-08-01 |
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| series | Microorganisms |
| spelling | doaj.art-003b180b845c449ba12c7a0d3134949b2023-11-19T02:17:25ZengMDPI AGMicroorganisms2076-26072023-08-01118199610.3390/microorganisms11081996Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary HospitalMario Galindo-Méndez0Humberto Navarrete-Salazar1Reinaldo Pacheco-Vásquez2Devanhí Quintas-de la Paz3Isabel Baltazar-Jiménez4José David Santiago-Luna5Laura Guadarrama-Monroy6Laboratorios Galindo SC, Av Juárez 501-A, Col Centro, Oaxaca 68000, Oax, MexicoSchool of Medicine, Universidad Anáhuac Oaxaca, Blvd. Guadalupe Hinojosa de Murat 1100, San Raymundo Jalpam 71248, Oax, MexicoCentro Médico ISSEMYM Toluca, Av. Baja Velocidad KM. 57.5, Carr. Mex./Tol. Col. San Jeronimo Chicahualco, Metepec 52170, Edomex, MexicoSchool of Medicine, Universidad Anáhuac Oaxaca, Blvd. Guadalupe Hinojosa de Murat 1100, San Raymundo Jalpam 71248, Oax, MexicoSchool of Medicine, Universidad Anáhuac Oaxaca, Blvd. Guadalupe Hinojosa de Murat 1100, San Raymundo Jalpam 71248, Oax, MexicoSchool of Medicine, Universidad Anáhuac Oaxaca, Blvd. Guadalupe Hinojosa de Murat 1100, San Raymundo Jalpam 71248, Oax, MexicoCentro Médico ISSEMYM Toluca, Av. Baja Velocidad KM. 57.5, Carr. Mex./Tol. Col. San Jeronimo Chicahualco, Metepec 52170, Edomex, MexicoThe aim of this study was to determine the prevalence of plasmid-mediated colistin resistance <i>mcr</i>-1 to <i>mcr</i>-5 genes among colistin and multi-drug-resistant Gram-negative bacilli strains isolated from patients in a tertiary hospital in Toluca, Mexico. The presence of <i>mcr</i> genes among the 241 strains collected was assessed by PCR. In the case of <i>mcr</i>-carrying <i>E. coli</i>, further PCR tests were performed to determine the presence of <i>bla</i><sub>CTX-M</sub> and whether the strains belonged to the O25b-ST131 clone. Conjugation experiments were also carried out to assess the horizontal transmission of colistin resistance. A total of twelve strains (5.0%), of which four were <i>E. coli</i>; four were <i>P. aeruginosa</i>; three were <i>K. pneumoniae,</i> and one <i>E. cloacae</i>, were found to be resistant to colistin. Of these strains, two <i>E. coli</i> isolates were found to carry <i>mcr</i>-1, and Southern blot hybridization demonstrated its presence on an approximately 60 kb plasmid. Both <i>mcr</i>-1-carrying <i>E. coli</i> strains were found to co-express <i>bla</i><sub>CTX-M</sub>, belong to the O25b-ST131 clone, and horizontally transmit their colistin resistance. The results of this study confirm the presence of plasmid-mediated colistin resistance in hospitalized patients in Mexico and demonstrated that the multi-drug-resistant O25b-ST131 <i>E. coli</i> clone can acquire <i>mcr</i> genes and transmit such resistance traits to other bacteria.https://www.mdpi.com/2076-2607/11/8/1996antibiotic resistance<i>mcr</i>-1plasmid-mediated colistin resistanceO25b-ST131CTX-M |
| spellingShingle | Mario Galindo-Méndez Humberto Navarrete-Salazar Reinaldo Pacheco-Vásquez Devanhí Quintas-de la Paz Isabel Baltazar-Jiménez José David Santiago-Luna Laura Guadarrama-Monroy Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital Microorganisms antibiotic resistance <i>mcr</i>-1 plasmid-mediated colistin resistance O25b-ST131 CTX-M |
| title | Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital |
| title_full | Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital |
| title_fullStr | Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital |
| title_full_unstemmed | Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital |
| title_short | Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital |
| title_sort | detection of plasmid mediated resistance against colistin in multi drug resistant gram negative bacilli isolated from a tertiary hospital |
| topic | antibiotic resistance <i>mcr</i>-1 plasmid-mediated colistin resistance O25b-ST131 CTX-M |
| url | https://www.mdpi.com/2076-2607/11/8/1996 |
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