Dichloromethane fraction of Citrus grandis induces apoptosis in a human colorectal cancer cell lines via apoptotic signaling pathway
The incidence of colorectal cancer is rapidly growing in Korea, due to increasingly westernized dietary. We investigated the inhibitory effects of Citrus grandis extracts rich in polymethoxylated flavones in colorectal cancer. CGDF treatment changed the ratio of pro-apoptotic and anti-apoptotic Bcl-...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464621005521 |
| _version_ | 1818733155684712448 |
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| author | Taekil Eom Jae Ho Choi Jungman Kim Jusung Kim Tatsuya Unno |
| author_facet | Taekil Eom Jae Ho Choi Jungman Kim Jusung Kim Tatsuya Unno |
| author_sort | Taekil Eom |
| collection | DOAJ |
| description | The incidence of colorectal cancer is rapidly growing in Korea, due to increasingly westernized dietary. We investigated the inhibitory effects of Citrus grandis extracts rich in polymethoxylated flavones in colorectal cancer. CGDF treatment changed the ratio of pro-apoptotic and anti-apoptotic Bcl-2 family proteins and generated ROS. CGDF treatment could promote the release of cytochrome c into the cytosol by activating the expression of caspases and decreasing the expression of IAPs. ROS generated by CGDF may initiate upstream signaling in the apoptotic signaling pathway. CGDF treatment inhibited the growth of xenograft tumors in mice. Gut microbiota analysis showed significant intestinal microbial shifts caused by CGDF ingestion, in which SCFA producers, Clostridium_IV, Oscillibacter, and Acetatifactor, were increased, while the number of potentially tumorigenic bacteria Staphylococcus was decreased. Therefore, we suggest that C. grandis peel extract and its fractions promote the mitochondrial apoptotic cascade in human colorectal cells by shifting the gut microbiota. |
| first_indexed | 2024-12-17T23:44:58Z |
| format | Article |
| id | doaj.art-0041ff43816c43b29b283985ab8ea28d |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-17T23:44:58Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-0041ff43816c43b29b283985ab8ea28d2022-12-21T21:28:20ZengElsevierJournal of Functional Foods1756-46462022-01-0188104903Dichloromethane fraction of Citrus grandis induces apoptosis in a human colorectal cancer cell lines via apoptotic signaling pathwayTaekil Eom0Jae Ho Choi1Jungman Kim2Jusung Kim3Tatsuya Unno4Subtropical/Tropical Organism Gene Bank, SARI, Jeju National University, Jeju 63243, Republic of KoreaSubtropical/Tropical Organism Gene Bank, SARI, Jeju National University, Jeju 63243, Republic of KoreaFaculty of Biotechnology, School of Life Sciences, SARI, Jeju National University, Jeju 63243, Republic of KoreaMajors in Plant Resource and Environment, College of Agriculture & Life Sciences, SARI, Jeju National University, Jeju 63243, Republic of KoreaSubtropical/Tropical Organism Gene Bank, SARI, Jeju National University, Jeju 63243, Republic of Korea; Faculty of Biotechnology, School of Life Sciences, SARI, Jeju National University, Jeju 63243, Republic of Korea; Corresponding author at: Subtropical/Tropical Organism Gene Bank, SARI, Jeju National University, Jeju 63243, Republic of Korea; Faculty of Biotechnology, School of Life Sciences, SARI, Jeju National University, Jeju 63243, Korea.The incidence of colorectal cancer is rapidly growing in Korea, due to increasingly westernized dietary. We investigated the inhibitory effects of Citrus grandis extracts rich in polymethoxylated flavones in colorectal cancer. CGDF treatment changed the ratio of pro-apoptotic and anti-apoptotic Bcl-2 family proteins and generated ROS. CGDF treatment could promote the release of cytochrome c into the cytosol by activating the expression of caspases and decreasing the expression of IAPs. ROS generated by CGDF may initiate upstream signaling in the apoptotic signaling pathway. CGDF treatment inhibited the growth of xenograft tumors in mice. Gut microbiota analysis showed significant intestinal microbial shifts caused by CGDF ingestion, in which SCFA producers, Clostridium_IV, Oscillibacter, and Acetatifactor, were increased, while the number of potentially tumorigenic bacteria Staphylococcus was decreased. Therefore, we suggest that C. grandis peel extract and its fractions promote the mitochondrial apoptotic cascade in human colorectal cells by shifting the gut microbiota.http://www.sciencedirect.com/science/article/pii/S1756464621005521Citrus grandis (L.) OsbeckCGDFColorectal cancerApoptosis, Gut microbiota |
| spellingShingle | Taekil Eom Jae Ho Choi Jungman Kim Jusung Kim Tatsuya Unno Dichloromethane fraction of Citrus grandis induces apoptosis in a human colorectal cancer cell lines via apoptotic signaling pathway Journal of Functional Foods Citrus grandis (L.) Osbeck CGDF Colorectal cancer Apoptosis, Gut microbiota |
| title | Dichloromethane fraction of Citrus grandis induces apoptosis in a human colorectal cancer cell lines via apoptotic signaling pathway |
| title_full | Dichloromethane fraction of Citrus grandis induces apoptosis in a human colorectal cancer cell lines via apoptotic signaling pathway |
| title_fullStr | Dichloromethane fraction of Citrus grandis induces apoptosis in a human colorectal cancer cell lines via apoptotic signaling pathway |
| title_full_unstemmed | Dichloromethane fraction of Citrus grandis induces apoptosis in a human colorectal cancer cell lines via apoptotic signaling pathway |
| title_short | Dichloromethane fraction of Citrus grandis induces apoptosis in a human colorectal cancer cell lines via apoptotic signaling pathway |
| title_sort | dichloromethane fraction of citrus grandis induces apoptosis in a human colorectal cancer cell lines via apoptotic signaling pathway |
| topic | Citrus grandis (L.) Osbeck CGDF Colorectal cancer Apoptosis, Gut microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S1756464621005521 |
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