Cardioprotective effects of Fenugreek (Trigonella foenum-graceum) seed extract in streptozotocin induced diabetic rats
Introduction: Inadequate control of diabetes mellitus (DM) leads to considerable cardiovascular implications like diabetic cardiomyopathy (DCM). Cardiomyocyte apoptosis is one of the main mechanisms of DCM pathogenesis associated with hyperglycemia, oxidative stress, inflammation, hyperlipidemia and...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2021-02-01
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| Series: | Journal of Cardiovascular and Thoracic Research |
| Subjects: | |
| Online Access: | https://jcvtr.tbzmed.ac.ir/PDF/jcvtr-13-28.pdf |
| _version_ | 1819004466262704128 |
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| author | Soleyman Bafadam Maryam Mahmoudabady Saeed Niazmand Seyed Abdolrahim Rezaee Mohammad Soukhtanloo |
| author_facet | Soleyman Bafadam Maryam Mahmoudabady Saeed Niazmand Seyed Abdolrahim Rezaee Mohammad Soukhtanloo |
| author_sort | Soleyman Bafadam |
| collection | DOAJ |
| description | Introduction: Inadequate control of diabetes mellitus (DM) leads to considerable cardiovascular implications like diabetic cardiomyopathy (DCM). Cardiomyocyte apoptosis is one of the main mechanisms of DCM pathogenesis associated with hyperglycemia, oxidative stress, inflammation, hyperlipidemia and several other factors. Trigonella foenum-graecum (Fenugreek) has been long used as a traditional medicine and has many therapeutic effects, including anti-diabetic, anti-hyperlipidemia, anti-inflammatory and anti-oxidant properties. The current study aimed to investigate cardioprotective effects of fenugreek seed on diabetic rats. Methods: Diabetes was induced in forty-two male rats by injection of streptozotocin (STZ) (60 mg/ kg). Diabetic animals were treated with three different doses of fenugreek seed extract (50, 100 and 200 mg/kg) or metformin (300 mg/kg) for six weeks by gavage. Nondiabetic rats served as controls. Glucose, cholesterol, and triglycerides levels were measured in the blood samples, and oxidative stress markers as well as gene expression of ICAM1, Bax and Bcl2 were assessed in the cardiac tissues of the experimental groups. Results: Diabetic rats exhibited increased serum glucose, cholesterol and triglycerides levels, elevated markers of oxidative stress thiobarbituric acid–reacting substances (TBARS) levels , total thiol groups (SH), catalase (CAT) and superoxide dismutase (SOD) activity, and enhanced apoptosis cell death (ratio of Bax/Bcl2). Fenugreek seed extract considerably improved metabolism abnormalities, attenuated oxidative stress and diminished apoptosis index. Conclusion: Our study suggests that fenugreek seed may protect the cardiac structure in STZ-induced diabetic rats by attenuating oxidative stress and apoptosis. |
| first_indexed | 2024-12-20T23:37:20Z |
| format | Article |
| id | doaj.art-004206b6858f41bdbc91ff74b87cd302 |
| institution | Directory Open Access Journal |
| issn | 2008-5117 2008-6830 |
| language | English |
| last_indexed | 2024-12-20T23:37:20Z |
| publishDate | 2021-02-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Journal of Cardiovascular and Thoracic Research |
| spelling | doaj.art-004206b6858f41bdbc91ff74b87cd3022022-12-21T19:23:10ZengTabriz University of Medical SciencesJournal of Cardiovascular and Thoracic Research2008-51172008-68302021-02-01131283610.34172/jcvtr.2021.01jcvtr-28804Cardioprotective effects of Fenugreek (Trigonella foenum-graceum) seed extract in streptozotocin induced diabetic ratsSoleyman Bafadam0Maryam Mahmoudabady1Saeed Niazmand2Seyed Abdolrahim Rezaee3Mohammad Soukhtanloo4Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranImmunology Research Center, Inflammation and Inflammatory Diseases Division, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranIntroduction: Inadequate control of diabetes mellitus (DM) leads to considerable cardiovascular implications like diabetic cardiomyopathy (DCM). Cardiomyocyte apoptosis is one of the main mechanisms of DCM pathogenesis associated with hyperglycemia, oxidative stress, inflammation, hyperlipidemia and several other factors. Trigonella foenum-graecum (Fenugreek) has been long used as a traditional medicine and has many therapeutic effects, including anti-diabetic, anti-hyperlipidemia, anti-inflammatory and anti-oxidant properties. The current study aimed to investigate cardioprotective effects of fenugreek seed on diabetic rats. Methods: Diabetes was induced in forty-two male rats by injection of streptozotocin (STZ) (60 mg/ kg). Diabetic animals were treated with three different doses of fenugreek seed extract (50, 100 and 200 mg/kg) or metformin (300 mg/kg) for six weeks by gavage. Nondiabetic rats served as controls. Glucose, cholesterol, and triglycerides levels were measured in the blood samples, and oxidative stress markers as well as gene expression of ICAM1, Bax and Bcl2 were assessed in the cardiac tissues of the experimental groups. Results: Diabetic rats exhibited increased serum glucose, cholesterol and triglycerides levels, elevated markers of oxidative stress thiobarbituric acid–reacting substances (TBARS) levels , total thiol groups (SH), catalase (CAT) and superoxide dismutase (SOD) activity, and enhanced apoptosis cell death (ratio of Bax/Bcl2). Fenugreek seed extract considerably improved metabolism abnormalities, attenuated oxidative stress and diminished apoptosis index. Conclusion: Our study suggests that fenugreek seed may protect the cardiac structure in STZ-induced diabetic rats by attenuating oxidative stress and apoptosis.https://jcvtr.tbzmed.ac.ir/PDF/jcvtr-13-28.pdfdiabetesfenugreek seedcardiomyopathyoxidative stressapoptosis |
| spellingShingle | Soleyman Bafadam Maryam Mahmoudabady Saeed Niazmand Seyed Abdolrahim Rezaee Mohammad Soukhtanloo Cardioprotective effects of Fenugreek (Trigonella foenum-graceum) seed extract in streptozotocin induced diabetic rats Journal of Cardiovascular and Thoracic Research diabetes fenugreek seed cardiomyopathy oxidative stress apoptosis |
| title | Cardioprotective effects of Fenugreek (Trigonella foenum-graceum) seed extract in streptozotocin induced diabetic rats |
| title_full | Cardioprotective effects of Fenugreek (Trigonella foenum-graceum) seed extract in streptozotocin induced diabetic rats |
| title_fullStr | Cardioprotective effects of Fenugreek (Trigonella foenum-graceum) seed extract in streptozotocin induced diabetic rats |
| title_full_unstemmed | Cardioprotective effects of Fenugreek (Trigonella foenum-graceum) seed extract in streptozotocin induced diabetic rats |
| title_short | Cardioprotective effects of Fenugreek (Trigonella foenum-graceum) seed extract in streptozotocin induced diabetic rats |
| title_sort | cardioprotective effects of fenugreek trigonella foenum graceum seed extract in streptozotocin induced diabetic rats |
| topic | diabetes fenugreek seed cardiomyopathy oxidative stress apoptosis |
| url | https://jcvtr.tbzmed.ac.ir/PDF/jcvtr-13-28.pdf |
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