<i>Aerococcus viridans</i> Phage Lysin AVPL Had Lytic Activity against <i>Streptococcus suis</i> in a Mouse Bacteremia Model
<i>Streptococcus suis</i> (<i>S. suis</i>) is a swine pathogen that can cause sepsis, meningitis, endocarditis, and other infectious diseases; it is also a zoonotic pathogen that has caused a global surge in fatal human infections. The widespread prevalence of multidrug-resis...
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MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/23/16670 |
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| author | Hengyu Xi Yao Fu Chong Chen Xin Feng Wenyu Han Jingmin Gu Yalu Ji |
| author_facet | Hengyu Xi Yao Fu Chong Chen Xin Feng Wenyu Han Jingmin Gu Yalu Ji |
| author_sort | Hengyu Xi |
| collection | DOAJ |
| description | <i>Streptococcus suis</i> (<i>S. suis</i>) is a swine pathogen that can cause sepsis, meningitis, endocarditis, and other infectious diseases; it is also a zoonotic pathogen that has caused a global surge in fatal human infections. The widespread prevalence of multidrug-resistant <i>S. suis</i> strains and the decline in novel antibiotic candidates have necessitated the development of alternative antimicrobial agents. In this study, AVPL, the <i>Aerococcus viridans</i> (<i>A. viridans</i>) phage lysin, was found to exhibit efficient bactericidal activity and broad lytic activity against multiple serotypes of <i>S. suis</i>. A final concentration of 300 μg/mL AVPL reduced <i>S. suis</i> counts by 4–4.5 log10 within 1 h in vitro. Importantly, AVPL effectively inhibited 48 h <i>S. suis</i> biofilm formation and disrupted preformed biofilms. In a mouse model, 300 μg/mouse AVPL protected 100% of mice from infection following the administration of lethal doses of multidrug-resistant <i>S. suis</i> type 2 (SS2) strain SC19, reduced the bacterial load in different organs, and effectively alleviated inflammation and histopathological damage in infected mice. These data suggest that AVPL is a valuable candidate antimicrobial agent for treating <i>S. suis</i> infections. |
| first_indexed | 2024-03-09T01:50:27Z |
| format | Article |
| id | doaj.art-00426662298341abb578966169398080 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T01:50:27Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-00426662298341abb5789661693980802023-12-08T15:16:42ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124231667010.3390/ijms242316670<i>Aerococcus viridans</i> Phage Lysin AVPL Had Lytic Activity against <i>Streptococcus suis</i> in a Mouse Bacteremia ModelHengyu Xi0Yao Fu1Chong Chen2Xin Feng3Wenyu Han4Jingmin Gu5Yalu Ji6State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China<i>Streptococcus suis</i> (<i>S. suis</i>) is a swine pathogen that can cause sepsis, meningitis, endocarditis, and other infectious diseases; it is also a zoonotic pathogen that has caused a global surge in fatal human infections. The widespread prevalence of multidrug-resistant <i>S. suis</i> strains and the decline in novel antibiotic candidates have necessitated the development of alternative antimicrobial agents. In this study, AVPL, the <i>Aerococcus viridans</i> (<i>A. viridans</i>) phage lysin, was found to exhibit efficient bactericidal activity and broad lytic activity against multiple serotypes of <i>S. suis</i>. A final concentration of 300 μg/mL AVPL reduced <i>S. suis</i> counts by 4–4.5 log10 within 1 h in vitro. Importantly, AVPL effectively inhibited 48 h <i>S. suis</i> biofilm formation and disrupted preformed biofilms. In a mouse model, 300 μg/mouse AVPL protected 100% of mice from infection following the administration of lethal doses of multidrug-resistant <i>S. suis</i> type 2 (SS2) strain SC19, reduced the bacterial load in different organs, and effectively alleviated inflammation and histopathological damage in infected mice. These data suggest that AVPL is a valuable candidate antimicrobial agent for treating <i>S. suis</i> infections.https://www.mdpi.com/1422-0067/24/23/16670<i>Streptococcus suis</i><i>Aerococcus viridans</i>biofilmbacteremiaphage lysin |
| spellingShingle | Hengyu Xi Yao Fu Chong Chen Xin Feng Wenyu Han Jingmin Gu Yalu Ji <i>Aerococcus viridans</i> Phage Lysin AVPL Had Lytic Activity against <i>Streptococcus suis</i> in a Mouse Bacteremia Model International Journal of Molecular Sciences <i>Streptococcus suis</i> <i>Aerococcus viridans</i> biofilm bacteremia phage lysin |
| title | <i>Aerococcus viridans</i> Phage Lysin AVPL Had Lytic Activity against <i>Streptococcus suis</i> in a Mouse Bacteremia Model |
| title_full | <i>Aerococcus viridans</i> Phage Lysin AVPL Had Lytic Activity against <i>Streptococcus suis</i> in a Mouse Bacteremia Model |
| title_fullStr | <i>Aerococcus viridans</i> Phage Lysin AVPL Had Lytic Activity against <i>Streptococcus suis</i> in a Mouse Bacteremia Model |
| title_full_unstemmed | <i>Aerococcus viridans</i> Phage Lysin AVPL Had Lytic Activity against <i>Streptococcus suis</i> in a Mouse Bacteremia Model |
| title_short | <i>Aerococcus viridans</i> Phage Lysin AVPL Had Lytic Activity against <i>Streptococcus suis</i> in a Mouse Bacteremia Model |
| title_sort | i aerococcus viridans i phage lysin avpl had lytic activity against i streptococcus suis i in a mouse bacteremia model |
| topic | <i>Streptococcus suis</i> <i>Aerococcus viridans</i> biofilm bacteremia phage lysin |
| url | https://www.mdpi.com/1422-0067/24/23/16670 |
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