Expression of Renal Vitamin D and Phosphatonin-Related Genes in a Sheep Model of Osteoporosis
Osteoporosis is a significant public health issue around the world, with post-menopausal osteoporosis due to estrogen deficiency resulting in approximately ¾ of cases. In this study, 18 aged Merino ewes were ovariectomized, and 10 were controls. Three of the ovariectomized ewes were treated weekly w...
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MDPI AG
2021-12-01
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| author | Keren E. Dittmer Anastasia Chernyavtseva Jonathan C. Marshall Diana Cabrera Frances M. Wolber Marlena Kruger |
| author_facet | Keren E. Dittmer Anastasia Chernyavtseva Jonathan C. Marshall Diana Cabrera Frances M. Wolber Marlena Kruger |
| author_sort | Keren E. Dittmer |
| collection | DOAJ |
| description | Osteoporosis is a significant public health issue around the world, with post-menopausal osteoporosis due to estrogen deficiency resulting in approximately ¾ of cases. In this study, 18 aged Merino ewes were ovariectomized, and 10 were controls. Three of the ovariectomized ewes were treated weekly with 400 mg of methylprednisolone for 5 months and three were treated weekly for 2 months, followed by a 3-month recovery period. At 2 months, five control animals and six ovariectomized animals were euthanized. At 5 months, all the remaining ewes were euthanized. Kidney samples were collected postmortem for qPCR analysis of <i>NPT1</i>, <i>PTH1R</i>, <i>NPT2a</i>, <i>NPT2c</i>, <i>Klotho</i>, <i>FGFR1IIIc</i>, <i>VDR</i>, <i>CYP24A1</i>, <i>CYP27B1</i>, <i>TRPV5</i>, <i>TRPV6</i>, <i>CalD9k</i>, <i>CalD28k</i>, <i>PMCA</i> and <i>NCX1.</i> Ovariectomized sheep had significantly greater <i>VDR</i> expression compared with other groups. Ovariectomized sheep treated with glucocorticoids for 2 months followed by euthanasia at 5 months showed significant differences in <i>TRPV5</i>, <i>CYP24A1</i> and <i>klotho</i> gene expression compared to other groups. Differences in <i>klotho</i> expression were most marked after adjustment for repeated measures (<i>p</i> = 0.1). <i>Klotho</i> is known as the “anti-aging” hormone and is involved in calcium and phosphorus metabolism. <i>Klotho</i> may be involved in the recovery of bone mineral density in ovariectomized sheep treated with glucocorticoids for 2 months followed by euthanasia at 5 months. Further research on the role of <i>klotho</i> is recommended. |
| first_indexed | 2024-03-10T03:50:50Z |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T03:50:50Z |
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| spelling | doaj.art-00430c671c624504af0307c6115fc0bf2023-11-23T11:05:14ZengMDPI AGAnimals2076-26152021-12-011216710.3390/ani12010067Expression of Renal Vitamin D and Phosphatonin-Related Genes in a Sheep Model of OsteoporosisKeren E. Dittmer0Anastasia Chernyavtseva1Jonathan C. Marshall2Diana Cabrera3Frances M. Wolber4Marlena Kruger5School of Veterinary Science, Massey University, Palmerston North 4442, New ZealandSchool of Veterinary Science, Massey University, Palmerston North 4442, New ZealandSchool of Fundamental Sciences, Massey University, Palmerston North 4442, New ZealandSchool of Food and Advanced Technology, Massey University, Palmerston North 4442, New ZealandSchool of Food and Advanced Technology, Massey University, Palmerston North 4442, New ZealandSchool of Health Sciences, Massey University, Palmerston North 4442, New ZealandOsteoporosis is a significant public health issue around the world, with post-menopausal osteoporosis due to estrogen deficiency resulting in approximately ¾ of cases. In this study, 18 aged Merino ewes were ovariectomized, and 10 were controls. Three of the ovariectomized ewes were treated weekly with 400 mg of methylprednisolone for 5 months and three were treated weekly for 2 months, followed by a 3-month recovery period. At 2 months, five control animals and six ovariectomized animals were euthanized. At 5 months, all the remaining ewes were euthanized. Kidney samples were collected postmortem for qPCR analysis of <i>NPT1</i>, <i>PTH1R</i>, <i>NPT2a</i>, <i>NPT2c</i>, <i>Klotho</i>, <i>FGFR1IIIc</i>, <i>VDR</i>, <i>CYP24A1</i>, <i>CYP27B1</i>, <i>TRPV5</i>, <i>TRPV6</i>, <i>CalD9k</i>, <i>CalD28k</i>, <i>PMCA</i> and <i>NCX1.</i> Ovariectomized sheep had significantly greater <i>VDR</i> expression compared with other groups. Ovariectomized sheep treated with glucocorticoids for 2 months followed by euthanasia at 5 months showed significant differences in <i>TRPV5</i>, <i>CYP24A1</i> and <i>klotho</i> gene expression compared to other groups. Differences in <i>klotho</i> expression were most marked after adjustment for repeated measures (<i>p</i> = 0.1). <i>Klotho</i> is known as the “anti-aging” hormone and is involved in calcium and phosphorus metabolism. <i>Klotho</i> may be involved in the recovery of bone mineral density in ovariectomized sheep treated with glucocorticoids for 2 months followed by euthanasia at 5 months. Further research on the role of <i>klotho</i> is recommended.https://www.mdpi.com/2076-2615/12/1/67osteoporosissheepvitamin DphosphatoninqPCRcalcium metabolism |
| spellingShingle | Keren E. Dittmer Anastasia Chernyavtseva Jonathan C. Marshall Diana Cabrera Frances M. Wolber Marlena Kruger Expression of Renal Vitamin D and Phosphatonin-Related Genes in a Sheep Model of Osteoporosis Animals osteoporosis sheep vitamin D phosphatonin qPCR calcium metabolism |
| title | Expression of Renal Vitamin D and Phosphatonin-Related Genes in a Sheep Model of Osteoporosis |
| title_full | Expression of Renal Vitamin D and Phosphatonin-Related Genes in a Sheep Model of Osteoporosis |
| title_fullStr | Expression of Renal Vitamin D and Phosphatonin-Related Genes in a Sheep Model of Osteoporosis |
| title_full_unstemmed | Expression of Renal Vitamin D and Phosphatonin-Related Genes in a Sheep Model of Osteoporosis |
| title_short | Expression of Renal Vitamin D and Phosphatonin-Related Genes in a Sheep Model of Osteoporosis |
| title_sort | expression of renal vitamin d and phosphatonin related genes in a sheep model of osteoporosis |
| topic | osteoporosis sheep vitamin D phosphatonin qPCR calcium metabolism |
| url | https://www.mdpi.com/2076-2615/12/1/67 |
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