Screening of supports for immobilization of commercial porcine pancreatic lipase
The aim of this work is to report the performance of different supports for the immobilization of commercial porcine pancreatic lipase. The immobilization tests were carried out in several types of Accurel, activated alumina, kaolin, montmorillonite, ion exchange resins and zeolites. The characteriz...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímeros (ABPol)
2011-12-01
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| Series: | Materials Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392011000400010 |
| _version_ | 1811196735899303936 |
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| author | Robison Scherer J. Vladimir Oliveira Sibele Pergher Débora de Oliveira |
| author_facet | Robison Scherer J. Vladimir Oliveira Sibele Pergher Débora de Oliveira |
| author_sort | Robison Scherer |
| collection | DOAJ |
| description | The aim of this work is to report the performance of different supports for the immobilization of commercial porcine pancreatic lipase. The immobilization tests were carried out in several types of Accurel, activated alumina, kaolin, montmorillonite, ion exchange resins and zeolites. The characterization of the supports showed differences in terms of specific area and morphology. The characteristics of the supports influenced the amount of enzyme adsorbed, yield of immobilization and esterification activity of the resulting immobilized catalyst. The clays KSF and natural and pillared montmorillonites presented potential for use as support for lipase immobilization in terms of yield and esterification activity. Yields of immobilization of 76.32 and 52.01% were achieved for clays KSF and natural montmorillonite, respectively. Esterification activities of 754.03, 595.51, 591.88 and 515.71 U.g-1 were obtained for lipases immobilized in Accurel MP-100, Amberlite XAD-2, mordenite and pillared montmorillonite, respectively. |
| first_indexed | 2024-04-12T01:04:00Z |
| format | Article |
| id | doaj.art-00484fafe8dd4f07832ff70efd543b4b |
| institution | Directory Open Access Journal |
| issn | 1516-1439 |
| language | English |
| last_indexed | 2024-04-12T01:04:00Z |
| publishDate | 2011-12-01 |
| publisher | Associação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímeros (ABPol) |
| record_format | Article |
| series | Materials Research |
| spelling | doaj.art-00484fafe8dd4f07832ff70efd543b4b2022-12-22T03:54:20ZengAssociação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímeros (ABPol)Materials Research1516-14392011-12-01144483492Screening of supports for immobilization of commercial porcine pancreatic lipaseRobison SchererJ. Vladimir OliveiraSibele PergherDébora de OliveiraThe aim of this work is to report the performance of different supports for the immobilization of commercial porcine pancreatic lipase. The immobilization tests were carried out in several types of Accurel, activated alumina, kaolin, montmorillonite, ion exchange resins and zeolites. The characterization of the supports showed differences in terms of specific area and morphology. The characteristics of the supports influenced the amount of enzyme adsorbed, yield of immobilization and esterification activity of the resulting immobilized catalyst. The clays KSF and natural and pillared montmorillonites presented potential for use as support for lipase immobilization in terms of yield and esterification activity. Yields of immobilization of 76.32 and 52.01% were achieved for clays KSF and natural montmorillonite, respectively. Esterification activities of 754.03, 595.51, 591.88 and 515.71 U.g-1 were obtained for lipases immobilized in Accurel MP-100, Amberlite XAD-2, mordenite and pillared montmorillonite, respectively.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392011000400010porcine pancreatic lipaseimmobilizationscreeningsupports |
| spellingShingle | Robison Scherer J. Vladimir Oliveira Sibele Pergher Débora de Oliveira Screening of supports for immobilization of commercial porcine pancreatic lipase Materials Research porcine pancreatic lipase immobilization screening supports |
| title | Screening of supports for immobilization of commercial porcine pancreatic lipase |
| title_full | Screening of supports for immobilization of commercial porcine pancreatic lipase |
| title_fullStr | Screening of supports for immobilization of commercial porcine pancreatic lipase |
| title_full_unstemmed | Screening of supports for immobilization of commercial porcine pancreatic lipase |
| title_short | Screening of supports for immobilization of commercial porcine pancreatic lipase |
| title_sort | screening of supports for immobilization of commercial porcine pancreatic lipase |
| topic | porcine pancreatic lipase immobilization screening supports |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392011000400010 |
| work_keys_str_mv | AT robisonscherer screeningofsupportsforimmobilizationofcommercialporcinepancreaticlipase AT jvladimiroliveira screeningofsupportsforimmobilizationofcommercialporcinepancreaticlipase AT sibelepergher screeningofsupportsforimmobilizationofcommercialporcinepancreaticlipase AT deboradeoliveira screeningofsupportsforimmobilizationofcommercialporcinepancreaticlipase |