Allele Specific Expression (ASE) analysis between Bos Taurus and Bos Indicus cows using RNA-Seq data at SNP level and gene level
Abstract In the current study, allele specific expression analysis was performed in two subspecies cows (Bos taurus and Bos indicus) at SNP and gene levels. RNA-Seq data of 21,078,477 and 20940063 paired end reads from pooling of whole blood samples (Leukocyte) from 40 US Holstein (Bos Taurus) and 4...
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| Format: | Article |
| Language: | English |
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Academia Brasileira de Ciências
2021-05-01
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| Series: | Anais da Academia Brasileira de Ciências |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000500802&tlng=en |
| _version_ | 1819282516819836928 |
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| author | SHEIDA VARKOOHI MOHAMMAD HOSSEIN BANABAZI MOJGAN GHSEMI-SIAB |
| author_facet | SHEIDA VARKOOHI MOHAMMAD HOSSEIN BANABAZI MOJGAN GHSEMI-SIAB |
| author_sort | SHEIDA VARKOOHI |
| collection | DOAJ |
| description | Abstract In the current study, allele specific expression analysis was performed in two subspecies cows (Bos taurus and Bos indicus) at SNP and gene levels. RNA-Seq data of 21,078,477 and 20940063 paired end reads from pooling of whole blood samples (Leukocyte) from 40 US Holstein (Bos Taurus) and 45 Cholistani cows (Bos indicus) obtained from SRA database in NCBI. Quality control and trimming of row RNA-Seq data were processed by FASTQC and Trimmomatic softwares. The transcriptome was assembled by TopHat2 software in two cow’s population by aligning and mapping the RNA-Seq reads on bovine reference genome. The SNPs were discovered by Samtools software and ASE analysis was performed by Chi-square test. Results showed that 50183 and 137954 SNPs were discovered on the assembled transcriptome of Holstein and Cholistani cow samples, respectively, and 15308 SNPs were common in both breeds. 10158 SNPs from 50183 (20%) in Holstein and 31523 SNPs from 137954 (23%) in Cholistani cows were identified as ASE-SNPs. Reference allele and alternative allele count in Holstein and Cholistani cows were 3041 and 7155, respectively. Among 131 discovered SNPs in 41 genes with different expression in Holstein and Cholistani cows, 31 ASE-SNPs (5 in Holstein; 26 in Cholistani cows) were discovered. |
| first_indexed | 2024-12-24T01:16:50Z |
| format | Article |
| id | doaj.art-0049f769b026405a99bb450508ce573d |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-12-24T01:16:50Z |
| publishDate | 2021-05-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-0049f769b026405a99bb450508ce573d2022-12-21T17:22:45ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902021-05-0193310.1590/0001-3765202120191453Allele Specific Expression (ASE) analysis between Bos Taurus and Bos Indicus cows using RNA-Seq data at SNP level and gene levelSHEIDA VARKOOHIhttps://orcid.org/0000-0002-7911-673XMOHAMMAD HOSSEIN BANABAZIhttps://orcid.org/0000-0002-7873-8123MOJGAN GHSEMI-SIABhttps://orcid.org/0000-0002-9674-0550Abstract In the current study, allele specific expression analysis was performed in two subspecies cows (Bos taurus and Bos indicus) at SNP and gene levels. RNA-Seq data of 21,078,477 and 20940063 paired end reads from pooling of whole blood samples (Leukocyte) from 40 US Holstein (Bos Taurus) and 45 Cholistani cows (Bos indicus) obtained from SRA database in NCBI. Quality control and trimming of row RNA-Seq data were processed by FASTQC and Trimmomatic softwares. The transcriptome was assembled by TopHat2 software in two cow’s population by aligning and mapping the RNA-Seq reads on bovine reference genome. The SNPs were discovered by Samtools software and ASE analysis was performed by Chi-square test. Results showed that 50183 and 137954 SNPs were discovered on the assembled transcriptome of Holstein and Cholistani cow samples, respectively, and 15308 SNPs were common in both breeds. 10158 SNPs from 50183 (20%) in Holstein and 31523 SNPs from 137954 (23%) in Cholistani cows were identified as ASE-SNPs. Reference allele and alternative allele count in Holstein and Cholistani cows were 3041 and 7155, respectively. Among 131 discovered SNPs in 41 genes with different expression in Holstein and Cholistani cows, 31 ASE-SNPs (5 in Holstein; 26 in Cholistani cows) were discovered.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000500802&tlng=enSNP discoverytranscriptomeCholistani cowsHolstein cows |
| spellingShingle | SHEIDA VARKOOHI MOHAMMAD HOSSEIN BANABAZI MOJGAN GHSEMI-SIAB Allele Specific Expression (ASE) analysis between Bos Taurus and Bos Indicus cows using RNA-Seq data at SNP level and gene level Anais da Academia Brasileira de Ciências SNP discovery transcriptome Cholistani cows Holstein cows |
| title | Allele Specific Expression (ASE) analysis between Bos Taurus and Bos Indicus cows using RNA-Seq data at SNP level and gene level |
| title_full | Allele Specific Expression (ASE) analysis between Bos Taurus and Bos Indicus cows using RNA-Seq data at SNP level and gene level |
| title_fullStr | Allele Specific Expression (ASE) analysis between Bos Taurus and Bos Indicus cows using RNA-Seq data at SNP level and gene level |
| title_full_unstemmed | Allele Specific Expression (ASE) analysis between Bos Taurus and Bos Indicus cows using RNA-Seq data at SNP level and gene level |
| title_short | Allele Specific Expression (ASE) analysis between Bos Taurus and Bos Indicus cows using RNA-Seq data at SNP level and gene level |
| title_sort | allele specific expression ase analysis between bos taurus and bos indicus cows using rna seq data at snp level and gene level |
| topic | SNP discovery transcriptome Cholistani cows Holstein cows |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000500802&tlng=en |
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