Analytical recommendations for SARS-CoV-2 identification by RT-PCR in pediatric patients

Coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome 2 coronavirus (SARS-CoV-2) and is currently listed as a global public health emergency. Timely identification and protocol implementations for molecular detection of this virus are vital for medical decision-makin...

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Main Authors: Israel Parra-Ortega, Armando Vilchis-Ordoñez, Briceida López-Martínez, Tania Ángeles-Floriano
Format: Article
Language:English
Published: Permanyer 2021-07-01
Series:Boletín Médico del Hospital Infantil de México
Subjects:
Online Access:https://www.bmhim.com/frame_esp.php?id=217
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author Israel Parra-Ortega
Armando Vilchis-Ordoñez
Briceida López-Martínez
Tania Ángeles-Floriano
author_facet Israel Parra-Ortega
Armando Vilchis-Ordoñez
Briceida López-Martínez
Tania Ángeles-Floriano
author_sort Israel Parra-Ortega
collection DOAJ
description Coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome 2 coronavirus (SARS-CoV-2) and is currently listed as a global public health emergency. Timely identification and protocol implementations for molecular detection of this virus are vital for medical decision-making. Identification of SARS-CoV-2 infection cases is based on detection of the virus RNA by molecular tests, particularly real-time reverse transcription-polymerase chain reaction (RT-PCR). Technical and operational details specific to each center must be considered to perform the molecular diagnosis of SARS-CoV-2 in pediatric patients. The term “qualified laboratories” involves laboratories in which all users, analysts, and anyone reporting results are trained to develop and interpret results through a procedure implemented previously by an instructor. Such knowledge is essential in detecting and identifying errors during each of its phases: pre-analytical, analytical, and post-analytical, which allow the establishment of continuous improvement policies to ensure the quality of the results, but above all, the physical integrity of health workers.
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spelling doaj.art-004e66e9f76d4851884304c1776ddf772022-12-21T20:17:43ZengPermanyerBoletín Médico del Hospital Infantil de México0539-61152021-07-0178310.24875/BMHIM.20000210Analytical recommendations for SARS-CoV-2 identification by RT-PCR in pediatric patientsIsrael Parra-Ortega0Armando Vilchis-Ordoñez1Briceida López-Martínez2Tania Ángeles-Floriano3Departamento de Laboratorio Clínico, Hospital Infantil de México Federico Gómez, Mexico City, MexicoDepartamento de Laboratorio Clínico, Hospital Infantil de México Federico Gómez, Mexico City, MexicoSubdirección de Servicios Auxiliares de Diagnóstico, Mexico City. MexicoDepartamento de Laboratorio Clínico, Hospital Infantil de México Federico Gómez, Mexico City, MexicoCoronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome 2 coronavirus (SARS-CoV-2) and is currently listed as a global public health emergency. Timely identification and protocol implementations for molecular detection of this virus are vital for medical decision-making. Identification of SARS-CoV-2 infection cases is based on detection of the virus RNA by molecular tests, particularly real-time reverse transcription-polymerase chain reaction (RT-PCR). Technical and operational details specific to each center must be considered to perform the molecular diagnosis of SARS-CoV-2 in pediatric patients. The term “qualified laboratories” involves laboratories in which all users, analysts, and anyone reporting results are trained to develop and interpret results through a procedure implemented previously by an instructor. Such knowledge is essential in detecting and identifying errors during each of its phases: pre-analytical, analytical, and post-analytical, which allow the establishment of continuous improvement policies to ensure the quality of the results, but above all, the physical integrity of health workers.https://www.bmhim.com/frame_esp.php?id=217COVID-19. SARS-CoV-2. RT-PCR.
spellingShingle Israel Parra-Ortega
Armando Vilchis-Ordoñez
Briceida López-Martínez
Tania Ángeles-Floriano
Analytical recommendations for SARS-CoV-2 identification by RT-PCR in pediatric patients
Boletín Médico del Hospital Infantil de México
COVID-19. SARS-CoV-2. RT-PCR.
title Analytical recommendations for SARS-CoV-2 identification by RT-PCR in pediatric patients
title_full Analytical recommendations for SARS-CoV-2 identification by RT-PCR in pediatric patients
title_fullStr Analytical recommendations for SARS-CoV-2 identification by RT-PCR in pediatric patients
title_full_unstemmed Analytical recommendations for SARS-CoV-2 identification by RT-PCR in pediatric patients
title_short Analytical recommendations for SARS-CoV-2 identification by RT-PCR in pediatric patients
title_sort analytical recommendations for sars cov 2 identification by rt pcr in pediatric patients
topic COVID-19. SARS-CoV-2. RT-PCR.
url https://www.bmhim.com/frame_esp.php?id=217
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AT briceidalopezmartinez analyticalrecommendationsforsarscov2identificationbyrtpcrinpediatricpatients
AT taniaangelesfloriano analyticalrecommendationsforsarscov2identificationbyrtpcrinpediatricpatients