A Virulence-Associated, 6.4-kb, Double-Stranded RNA from Rhizoctonia solani Is Phylogenetically Related to Plant Bromoviruses and Electron Transport Enzymes
We have recently shown that acquisition of a 6.4-kb, double-stranded (ds) RNA (M1) by hyphal anastomosis is associated with enhanced vigor and virulence, whereas its removal by hyphal tipping correlates with diminished virulence in the plant-pathogenic basidiomycete Rhizoctonia solani. The M1 dsRNA...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
1998-07-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1998.11.7.601 |
| _version_ | 1828235029100101632 |
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| author | JianHua Jian Dilip K. Lakshman Stellos M. Tavantzis |
| author_facet | JianHua Jian Dilip K. Lakshman Stellos M. Tavantzis |
| author_sort | JianHua Jian |
| collection | DOAJ |
| description | We have recently shown that acquisition of a 6.4-kb, double-stranded (ds) RNA (M1) by hyphal anastomosis is associated with enhanced vigor and virulence, whereas its removal by hyphal tipping correlates with diminished virulence in the plant-pathogenic basidiomycete Rhizoctonia solani. The M1 dsRNA is not encapsidated by a typical nucleocapsid, has a circular and/or concatemeric form, and is associated with the mitochondrial and cytosolic fractions. M1 possesses six open reading frames (ORFs) the longest of which (ORF 2) is located on the (+) strand, and encodes a putative polypeptide consisting of 1,747 amino acids or 199.4 kDa. This polypeptide has a significant amino acid sequence similarity, including six conserved helicase domains and an ATP/GTP binding motif, with the 1A protein of broad bean mottle virus (BBMV) and other bromoviruses. ORF 5, which is located on the (-) strand of M1 and is complementary to a region of ORF 2, codes for a putative polypeptide that has a significant amino acid sequence similarity with the cytochrome c oxidase assembly factor. This complementarity provides direct evidence suggesting that the long-standing hypothesis of viruses evolving from cellular genes may be valid. |
| first_indexed | 2024-04-12T20:06:32Z |
| format | Article |
| id | doaj.art-004e914082774d1098a2abbc16d162ba |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-12T20:06:32Z |
| publishDate | 1998-07-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-004e914082774d1098a2abbc16d162ba2022-12-22T03:18:23ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77061998-07-0111760160910.1094/MPMI.1998.11.7.601A Virulence-Associated, 6.4-kb, Double-Stranded RNA from Rhizoctonia solani Is Phylogenetically Related to Plant Bromoviruses and Electron Transport EnzymesJianHua JianDilip K. LakshmanStellos M. TavantzisWe have recently shown that acquisition of a 6.4-kb, double-stranded (ds) RNA (M1) by hyphal anastomosis is associated with enhanced vigor and virulence, whereas its removal by hyphal tipping correlates with diminished virulence in the plant-pathogenic basidiomycete Rhizoctonia solani. The M1 dsRNA is not encapsidated by a typical nucleocapsid, has a circular and/or concatemeric form, and is associated with the mitochondrial and cytosolic fractions. M1 possesses six open reading frames (ORFs) the longest of which (ORF 2) is located on the (+) strand, and encodes a putative polypeptide consisting of 1,747 amino acids or 199.4 kDa. This polypeptide has a significant amino acid sequence similarity, including six conserved helicase domains and an ATP/GTP binding motif, with the 1A protein of broad bean mottle virus (BBMV) and other bromoviruses. ORF 5, which is located on the (-) strand of M1 and is complementary to a region of ORF 2, codes for a putative polypeptide that has a significant amino acid sequence similarity with the cytochrome c oxidase assembly factor. This complementarity provides direct evidence suggesting that the long-standing hypothesis of viruses evolving from cellular genes may be valid.https://apsjournals.apsnet.org/doi/10.1094/MPMI.1998.11.7.601 |
| spellingShingle | JianHua Jian Dilip K. Lakshman Stellos M. Tavantzis A Virulence-Associated, 6.4-kb, Double-Stranded RNA from Rhizoctonia solani Is Phylogenetically Related to Plant Bromoviruses and Electron Transport Enzymes Molecular Plant-Microbe Interactions |
| title | A Virulence-Associated, 6.4-kb, Double-Stranded RNA from Rhizoctonia solani Is Phylogenetically Related to Plant Bromoviruses and Electron Transport Enzymes |
| title_full | A Virulence-Associated, 6.4-kb, Double-Stranded RNA from Rhizoctonia solani Is Phylogenetically Related to Plant Bromoviruses and Electron Transport Enzymes |
| title_fullStr | A Virulence-Associated, 6.4-kb, Double-Stranded RNA from Rhizoctonia solani Is Phylogenetically Related to Plant Bromoviruses and Electron Transport Enzymes |
| title_full_unstemmed | A Virulence-Associated, 6.4-kb, Double-Stranded RNA from Rhizoctonia solani Is Phylogenetically Related to Plant Bromoviruses and Electron Transport Enzymes |
| title_short | A Virulence-Associated, 6.4-kb, Double-Stranded RNA from Rhizoctonia solani Is Phylogenetically Related to Plant Bromoviruses and Electron Transport Enzymes |
| title_sort | virulence associated 6 4 kb double stranded rna from rhizoctonia solani is phylogenetically related to plant bromoviruses and electron transport enzymes |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1998.11.7.601 |
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