Protective effects of glaucocalyxin A on the airway of asthmatic mice
The aim of this study is to investigate the protective effects of glaucocalyxin A (GLA) on airways in mouse models of asthma, concerning the inflammatory mediators, Th1/Th2 subgroup imbalance, and Toll-like receptor 4 (TLR4)/NF-κB signaling pathway. Hematoxylin and eosin/periodic acid–Schiff stainin...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2022-07-01
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| Series: | Open Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/med-2022-0513 |
| _version_ | 1797998551842684928 |
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| author | Chen Si Piao Ying Song Yilan Wang Zhiguang Jiang Jingzhi Piao Yihua Li Li Xu Chang Li Liangchang Chi Yongxue Jin Guihua Yan Guanghai |
| author_facet | Chen Si Piao Ying Song Yilan Wang Zhiguang Jiang Jingzhi Piao Yihua Li Li Xu Chang Li Liangchang Chi Yongxue Jin Guihua Yan Guanghai |
| author_sort | Chen Si |
| collection | DOAJ |
| description | The aim of this study is to investigate the protective effects of glaucocalyxin A (GLA) on airways in mouse models of asthma, concerning the inflammatory mediators, Th1/Th2 subgroup imbalance, and Toll-like receptor 4 (TLR4)/NF-κB signaling pathway. Hematoxylin and eosin/periodic acid–Schiff staining was used to observe the pathological changes in lung tissues. Inflammatory cytokine contents in the bronchoalveolar lavage fluid were detected by enzyme-linked immunosorbent assay. Protein expression levels were detected with Western blot, immunohistochemistry, and immunofluorescence. In vivo studies showed that, in ovalbumin (OVA)-induced asthmatic mouse models, the GLA treatments reduced the airway hyperresponsiveness and the secretion of inflammatory cells, declined the proliferation of goblet cells, decreased the levels of IL-4, IL-5, and IL-13, and increased the contents of interferon-γ and IL-12. Moreover, GLA inhibited the protein expression levels of TLR4, MyD88, TRAF6, and NF-κB in OVA-induced asthmatic mouse models. Further in vitro studies showed that GLA inhibited the expression of NF-κB, p-IκBα, tumor necrosis factor-α, IL-6, and IL-1β and blocked the nuclear transfer of NF-κB in lipopolysaccharide-stimulated RAW264.7 macrophages. Conclusively, GLA can inhibit the inflammatory responses in OVA-induced asthmatic mice and inhibit the release of inflammatory factors in LPS-induced RAW264.7 macrophages, which may be related to the inhibition of TLR4/NF-κB signaling pathway. |
| first_indexed | 2024-04-11T10:50:32Z |
| format | Article |
| id | doaj.art-0050be93a3d54884ac5f498c08acd427 |
| institution | Directory Open Access Journal |
| issn | 2391-5463 |
| language | English |
| last_indexed | 2024-04-11T10:50:32Z |
| publishDate | 2022-07-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Medicine |
| spelling | doaj.art-0050be93a3d54884ac5f498c08acd4272022-12-22T04:28:55ZengDe GruyterOpen Medicine2391-54632022-07-011711158117110.1515/med-2022-0513Protective effects of glaucocalyxin A on the airway of asthmatic miceChen Si0Piao Ying1Song Yilan2Wang Zhiguang3Jiang Jingzhi4Piao Yihua5Li Li6Xu Chang7Li Liangchang8Chi Yongxue9Jin Guihua10Yan Guanghai11Department of Pediatrics, Affiliated Hospital of Yanbian University, Yanji 133099, Jilin, P. R. ChinaJilin Key Laboratory for Immune and Targeting Research on Common Allergic Diseases, Yanbian University, Yanji 133000, Jilin, P. R. ChinaJilin Key Laboratory for Immune and Targeting Research on Common Allergic Diseases, Yanbian University, Yanji 133000, Jilin, P. R. ChinaJilin Key Laboratory for Immune and Targeting Research on Common Allergic Diseases, Yanbian University, Yanji 133000, Jilin, P. R. ChinaJilin Key Laboratory for Immune and Targeting Research on Common Allergic Diseases, Yanbian University, Yanji 133000, Jilin, P. R. ChinaJilin Key Laboratory for Immune and Targeting Research on Common Allergic Diseases, Yanbian University, Yanji 133000, Jilin, P. R. ChinaJilin Key Laboratory for Immune and Targeting Research on Common Allergic Diseases, Yanbian University, Yanji 133000, Jilin, P. R. ChinaJilin Key Laboratory for Immune and Targeting Research on Common Allergic Diseases, Yanbian University, Yanji 133000, Jilin, P. R. ChinaJilin Key Laboratory for Immune and Targeting Research on Common Allergic Diseases, Yanbian University, Yanji 133000, Jilin, P. R. ChinaDepartment of Pediatrics, Affiliated Hospital of Yanbian University, No. 1327, Juzi Street, Yanji 133099, Jilin, P. R. ChinaJilin Key Laboratory for Immune and Targeting Research on Common Allergic Diseases, Yanbian University, Yanji 133000, Jilin, P. R. ChinaDepartment of Neonatology, Children’s Hospital of Changchun, Changchun 130061, Jilin, P. R. ChinaThe aim of this study is to investigate the protective effects of glaucocalyxin A (GLA) on airways in mouse models of asthma, concerning the inflammatory mediators, Th1/Th2 subgroup imbalance, and Toll-like receptor 4 (TLR4)/NF-κB signaling pathway. Hematoxylin and eosin/periodic acid–Schiff staining was used to observe the pathological changes in lung tissues. Inflammatory cytokine contents in the bronchoalveolar lavage fluid were detected by enzyme-linked immunosorbent assay. Protein expression levels were detected with Western blot, immunohistochemistry, and immunofluorescence. In vivo studies showed that, in ovalbumin (OVA)-induced asthmatic mouse models, the GLA treatments reduced the airway hyperresponsiveness and the secretion of inflammatory cells, declined the proliferation of goblet cells, decreased the levels of IL-4, IL-5, and IL-13, and increased the contents of interferon-γ and IL-12. Moreover, GLA inhibited the protein expression levels of TLR4, MyD88, TRAF6, and NF-κB in OVA-induced asthmatic mouse models. Further in vitro studies showed that GLA inhibited the expression of NF-κB, p-IκBα, tumor necrosis factor-α, IL-6, and IL-1β and blocked the nuclear transfer of NF-κB in lipopolysaccharide-stimulated RAW264.7 macrophages. Conclusively, GLA can inhibit the inflammatory responses in OVA-induced asthmatic mice and inhibit the release of inflammatory factors in LPS-induced RAW264.7 macrophages, which may be related to the inhibition of TLR4/NF-κB signaling pathway.https://doi.org/10.1515/med-2022-0513glaucocalyxin aasthmaairwaytlr4/nf-κb signaling pathwayinflammatory responses |
| spellingShingle | Chen Si Piao Ying Song Yilan Wang Zhiguang Jiang Jingzhi Piao Yihua Li Li Xu Chang Li Liangchang Chi Yongxue Jin Guihua Yan Guanghai Protective effects of glaucocalyxin A on the airway of asthmatic mice Open Medicine glaucocalyxin a asthma airway tlr4/nf-κb signaling pathway inflammatory responses |
| title | Protective effects of glaucocalyxin A on the airway of asthmatic mice |
| title_full | Protective effects of glaucocalyxin A on the airway of asthmatic mice |
| title_fullStr | Protective effects of glaucocalyxin A on the airway of asthmatic mice |
| title_full_unstemmed | Protective effects of glaucocalyxin A on the airway of asthmatic mice |
| title_short | Protective effects of glaucocalyxin A on the airway of asthmatic mice |
| title_sort | protective effects of glaucocalyxin a on the airway of asthmatic mice |
| topic | glaucocalyxin a asthma airway tlr4/nf-κb signaling pathway inflammatory responses |
| url | https://doi.org/10.1515/med-2022-0513 |
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