Analysis and Design of Stimulus Response Curves of E. coli
Metabolism and signalling are tightly coupled in bacteria. Combining several theoretical approaches, a core model is presented that describes transcriptional and allosteric control of glycolysis in Escherichia coli. Experimental data based on microarrays, signalling components and extracellular meta...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2012-11-01
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| Series: | Metabolites |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2218-1989/2/4/844 |
| _version_ | 1811305365346713600 |
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| author | Andreas Kremling Anna Goehler Knut Jahreis Markus Nees Benedikt Auerbach Wolfgang Schmidt-Heck Öznur Kökpinar Robert Geffers Ursula Rinas Katja Bettenbrock |
| author_facet | Andreas Kremling Anna Goehler Knut Jahreis Markus Nees Benedikt Auerbach Wolfgang Schmidt-Heck Öznur Kökpinar Robert Geffers Ursula Rinas Katja Bettenbrock |
| author_sort | Andreas Kremling |
| collection | DOAJ |
| description | Metabolism and signalling are tightly coupled in bacteria. Combining several theoretical approaches, a core model is presented that describes transcriptional and allosteric control of glycolysis in Escherichia coli. Experimental data based on microarrays, signalling components and extracellular metabolites are used to estimate kinetic parameters. A newly designed strain was used that adjusts the incoming glucose flux into the system and allows a kinetic analysis. Based on the results, prediction for intracelluar metabolite concentrations over a broad range of the growth rate could be performed and compared with data from literature. |
| first_indexed | 2024-04-13T08:25:15Z |
| format | Article |
| id | doaj.art-0052896b8f984fbe976ba087b35ee53a |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-04-13T08:25:15Z |
| publishDate | 2012-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-0052896b8f984fbe976ba087b35ee53a2022-12-22T02:54:30ZengMDPI AGMetabolites2218-19892012-11-012484487110.3390/metabo2040844Analysis and Design of Stimulus Response Curves of E. coliAndreas KremlingAnna GoehlerKnut JahreisMarkus NeesBenedikt AuerbachWolfgang Schmidt-HeckÖznur KökpinarRobert GeffersUrsula RinasKatja BettenbrockMetabolism and signalling are tightly coupled in bacteria. Combining several theoretical approaches, a core model is presented that describes transcriptional and allosteric control of glycolysis in Escherichia coli. Experimental data based on microarrays, signalling components and extracellular metabolites are used to estimate kinetic parameters. A newly designed strain was used that adjusts the incoming glucose flux into the system and allows a kinetic analysis. Based on the results, prediction for intracelluar metabolite concentrations over a broad range of the growth rate could be performed and compared with data from literature.http://www.mdpi.com/2218-1989/2/4/844carbohydrate uptakeEscherichia colinetwork component analysisfeedforward controlparameter estimationgene regulatory network |
| spellingShingle | Andreas Kremling Anna Goehler Knut Jahreis Markus Nees Benedikt Auerbach Wolfgang Schmidt-Heck Öznur Kökpinar Robert Geffers Ursula Rinas Katja Bettenbrock Analysis and Design of Stimulus Response Curves of E. coli Metabolites carbohydrate uptake Escherichia coli network component analysis feedforward control parameter estimation gene regulatory network |
| title | Analysis and Design of Stimulus Response Curves of E. coli |
| title_full | Analysis and Design of Stimulus Response Curves of E. coli |
| title_fullStr | Analysis and Design of Stimulus Response Curves of E. coli |
| title_full_unstemmed | Analysis and Design of Stimulus Response Curves of E. coli |
| title_short | Analysis and Design of Stimulus Response Curves of E. coli |
| title_sort | analysis and design of stimulus response curves of e coli |
| topic | carbohydrate uptake Escherichia coli network component analysis feedforward control parameter estimation gene regulatory network |
| url | http://www.mdpi.com/2218-1989/2/4/844 |
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