Dose-Response Recovery of Probiotic Strains in Simulated Gastro-Intestinal Passage
The probiotic definition stipulates “adequate amounts”. Here, we investigated the metabolic output and recovery rate of probiotic strains using a simulated upper gastro-intestinal passage and colonic fermentation. Two different doses, 7 × 10<sup>9</sup> colon...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/1/112 |
| _version_ | 1828414561711030272 |
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| author | Sofia Forssten Arthur C. Ouwehand |
| author_facet | Sofia Forssten Arthur C. Ouwehand |
| author_sort | Sofia Forssten |
| collection | DOAJ |
| description | The probiotic definition stipulates “adequate amounts”. Here, we investigated the metabolic output and recovery rate of probiotic strains using a simulated upper gastro-intestinal passage and colonic fermentation. Two different doses, 7 × 10<sup>9</sup> colony forming units (CFU) and 7 × 10<sup>10</sup> CFU, of a probiotic mixture (<i>Bifidobacterium lactis</i> Bl-04, <i>Lactobacillus acidophilus</i> La-14, <i>Lactobacillus paracasei</i> Lpc-37, and <i>Lactobacillus plantarum</i> Lp-115) or placebo were tested. The four strains were quantified by qPCR and the metabolites analyzed by gas chromatography. There was a dose-response in the detection of all four strains. There was a slightly larger increase between the two doses for <i>L. paracasei</i> Lpc-37 as compared with the other strains; this may suggest a greater robustness of this strain. Compared with the placebo, the high dose simulations generated more propionic acid and a higher total of short chain fatty acids (SCFA). Higher doses of a species are required to reach measurable increases above the baseline level of this species. |
| first_indexed | 2024-12-10T13:28:48Z |
| format | Article |
| id | doaj.art-005e4cf529944274b1b12d7dacf288aa |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-10T13:28:48Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-005e4cf529944274b1b12d7dacf288aa2022-12-22T01:47:03ZengMDPI AGMicroorganisms2076-26072020-01-018111210.3390/microorganisms8010112microorganisms8010112Dose-Response Recovery of Probiotic Strains in Simulated Gastro-Intestinal PassageSofia Forssten0Arthur C. Ouwehand1DuPont Nutrition and Biosciences, Sokeritehtaantie 20, FI-02460 Kantvik, FinlandDuPont Nutrition and Biosciences, Sokeritehtaantie 20, FI-02460 Kantvik, FinlandThe probiotic definition stipulates “adequate amounts”. Here, we investigated the metabolic output and recovery rate of probiotic strains using a simulated upper gastro-intestinal passage and colonic fermentation. Two different doses, 7 × 10<sup>9</sup> colony forming units (CFU) and 7 × 10<sup>10</sup> CFU, of a probiotic mixture (<i>Bifidobacterium lactis</i> Bl-04, <i>Lactobacillus acidophilus</i> La-14, <i>Lactobacillus paracasei</i> Lpc-37, and <i>Lactobacillus plantarum</i> Lp-115) or placebo were tested. The four strains were quantified by qPCR and the metabolites analyzed by gas chromatography. There was a dose-response in the detection of all four strains. There was a slightly larger increase between the two doses for <i>L. paracasei</i> Lpc-37 as compared with the other strains; this may suggest a greater robustness of this strain. Compared with the placebo, the high dose simulations generated more propionic acid and a higher total of short chain fatty acids (SCFA). Higher doses of a species are required to reach measurable increases above the baseline level of this species.https://www.mdpi.com/2076-2607/8/1/112<i>lactobacillus</i><i>bifidobacterium</i>probioticsimulated digestiondose-response |
| spellingShingle | Sofia Forssten Arthur C. Ouwehand Dose-Response Recovery of Probiotic Strains in Simulated Gastro-Intestinal Passage Microorganisms <i>lactobacillus</i> <i>bifidobacterium</i> probiotic simulated digestion dose-response |
| title | Dose-Response Recovery of Probiotic Strains in Simulated Gastro-Intestinal Passage |
| title_full | Dose-Response Recovery of Probiotic Strains in Simulated Gastro-Intestinal Passage |
| title_fullStr | Dose-Response Recovery of Probiotic Strains in Simulated Gastro-Intestinal Passage |
| title_full_unstemmed | Dose-Response Recovery of Probiotic Strains in Simulated Gastro-Intestinal Passage |
| title_short | Dose-Response Recovery of Probiotic Strains in Simulated Gastro-Intestinal Passage |
| title_sort | dose response recovery of probiotic strains in simulated gastro intestinal passage |
| topic | <i>lactobacillus</i> <i>bifidobacterium</i> probiotic simulated digestion dose-response |
| url | https://www.mdpi.com/2076-2607/8/1/112 |
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