The Use of Raman Spectroscopy to Monitor Metabolic Changes in Stressed <i>Metschnikowia</i> sp. Yeasts
Raman spectroscopy is a universal method designed for the analysis of a wide range of physical, chemical and biological systems or various surfaces. This technique is suitable to monitor various components of cells, tissues or microorganisms. The advantages include very fast non-contact and non-dest...
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MDPI AG
2021-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/2/277 |
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| author | Andrea Němcová Dominika Gonová Ota Samek Matthias Sipiczki Emilia Breierová Ivana Márová |
| author_facet | Andrea Němcová Dominika Gonová Ota Samek Matthias Sipiczki Emilia Breierová Ivana Márová |
| author_sort | Andrea Němcová |
| collection | DOAJ |
| description | Raman spectroscopy is a universal method designed for the analysis of a wide range of physical, chemical and biological systems or various surfaces. This technique is suitable to monitor various components of cells, tissues or microorganisms. The advantages include very fast non-contact and non-destructive analysis and no or minimal need for sample treatment. The yeasts <i>Metschnikowia</i> can be considered as industrially usable producers of pulcherrimin or single-cell lipids, depending on cultivation conditions and external stress. In the present study, Raman spectroscopy was used as an effective tool to identify both pulcherrimin and lipids in single yeast cells. The analysis of pulcherrimin is very demanding; so far, there is no optimal procedure to analyze or identify this pigment. Based on results, the strong dependence of pulcherrimin production on the ferric ion concentration was found with the highest yield in media containing 0.1 g/L iron. Further, production of lipids in <i>Metschnikowia</i> cells was studied at different temperatures and C:N ratios, using Raman spectroscopy to follow fatty acids composition, under different regimes, by monitoring the iodine number. The results of Raman spectroscopy were comparable with the fatty acid analysis obtained by gas chromatography. This study therefore supported use of Raman spectroscopy for biotechnological applications as a simple tool in the identification and analysis both the pulcherrimin and microbial lipids. This method provides a quick and relatively accurate estimation of targeted metabolites with minimal sample modification and allows to monitor metabolic changes over time of cultivation. |
| first_indexed | 2024-03-09T03:21:03Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T03:21:03Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-00604ae485914e3e904c462e24b56ed82023-12-03T15:10:10ZengMDPI AGMicroorganisms2076-26072021-01-019227710.3390/microorganisms9020277The Use of Raman Spectroscopy to Monitor Metabolic Changes in Stressed <i>Metschnikowia</i> sp. YeastsAndrea Němcová0Dominika Gonová1Ota Samek2Matthias Sipiczki3Emilia Breierová4Ivana Márová5Faculty of Chemistry, Brno University of Technology, Purkyňova 464/118, 612 00 Brno, Czech RepublicFaculty of Chemistry, Brno University of Technology, Purkyňova 464/118, 612 00 Brno, Czech RepublicInstitute of Scientific Instruments of the Czech Academy of Sciences, Královopolská 147, 612 64 Brno, Czech RepublicDepartment of Genetics and Applied Microbiology, Faculty of Science and Technology, University of Debrecen, Egyetem tér 1, 4032 Debrecen, HungaryInstitute of Chemistry, Slovak Academy of Sciences, Dúbravská Cesta 9, 845 38 Bratislava, SlovakiaFaculty of Chemistry, Brno University of Technology, Purkyňova 464/118, 612 00 Brno, Czech RepublicRaman spectroscopy is a universal method designed for the analysis of a wide range of physical, chemical and biological systems or various surfaces. This technique is suitable to monitor various components of cells, tissues or microorganisms. The advantages include very fast non-contact and non-destructive analysis and no or minimal need for sample treatment. The yeasts <i>Metschnikowia</i> can be considered as industrially usable producers of pulcherrimin or single-cell lipids, depending on cultivation conditions and external stress. In the present study, Raman spectroscopy was used as an effective tool to identify both pulcherrimin and lipids in single yeast cells. The analysis of pulcherrimin is very demanding; so far, there is no optimal procedure to analyze or identify this pigment. Based on results, the strong dependence of pulcherrimin production on the ferric ion concentration was found with the highest yield in media containing 0.1 g/L iron. Further, production of lipids in <i>Metschnikowia</i> cells was studied at different temperatures and C:N ratios, using Raman spectroscopy to follow fatty acids composition, under different regimes, by monitoring the iodine number. The results of Raman spectroscopy were comparable with the fatty acid analysis obtained by gas chromatography. This study therefore supported use of Raman spectroscopy for biotechnological applications as a simple tool in the identification and analysis both the pulcherrimin and microbial lipids. This method provides a quick and relatively accurate estimation of targeted metabolites with minimal sample modification and allows to monitor metabolic changes over time of cultivation.https://www.mdpi.com/2076-2607/9/2/277yeasts<i>Metschnikowia</i>lipidspulcherriminRaman spectroscopy |
| spellingShingle | Andrea Němcová Dominika Gonová Ota Samek Matthias Sipiczki Emilia Breierová Ivana Márová The Use of Raman Spectroscopy to Monitor Metabolic Changes in Stressed <i>Metschnikowia</i> sp. Yeasts Microorganisms yeasts <i>Metschnikowia</i> lipids pulcherrimin Raman spectroscopy |
| title | The Use of Raman Spectroscopy to Monitor Metabolic Changes in Stressed <i>Metschnikowia</i> sp. Yeasts |
| title_full | The Use of Raman Spectroscopy to Monitor Metabolic Changes in Stressed <i>Metschnikowia</i> sp. Yeasts |
| title_fullStr | The Use of Raman Spectroscopy to Monitor Metabolic Changes in Stressed <i>Metschnikowia</i> sp. Yeasts |
| title_full_unstemmed | The Use of Raman Spectroscopy to Monitor Metabolic Changes in Stressed <i>Metschnikowia</i> sp. Yeasts |
| title_short | The Use of Raman Spectroscopy to Monitor Metabolic Changes in Stressed <i>Metschnikowia</i> sp. Yeasts |
| title_sort | use of raman spectroscopy to monitor metabolic changes in stressed i metschnikowia i sp yeasts |
| topic | yeasts <i>Metschnikowia</i> lipids pulcherrimin Raman spectroscopy |
| url | https://www.mdpi.com/2076-2607/9/2/277 |
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