Protocol to utilize fresh uncultured human lung tumor cells for personalized functional diagnostics
Summary: Drug sensitivity data acquired from solid tumor-derived cultures are often unsuitable for personalized treatment guidance due to the lengthy turnaround time. Here, we present a protocol for determining ex vivo drug sensitivities using fresh uncultured human lung tumor-derived EpCAM+ epithel...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722006001 |
| _version_ | 1811199586668118016 |
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| author | Sarang S. Talwelkar Iris A.K. Lähdeniemi Mikko I. Mäyränpää Annabrita Hemmes Nora Linnavirta Jari Räsänen Aija Knuuttila Krister Wennerberg Emmy W. Verschuren |
| author_facet | Sarang S. Talwelkar Iris A.K. Lähdeniemi Mikko I. Mäyränpää Annabrita Hemmes Nora Linnavirta Jari Räsänen Aija Knuuttila Krister Wennerberg Emmy W. Verschuren |
| author_sort | Sarang S. Talwelkar |
| collection | DOAJ |
| description | Summary: Drug sensitivity data acquired from solid tumor-derived cultures are often unsuitable for personalized treatment guidance due to the lengthy turnaround time. Here, we present a protocol for determining ex vivo drug sensitivities using fresh uncultured human lung tumor-derived EpCAM+ epithelial cells (FUTCs). We describe steps for drug testing in FUTCs to identify tumor cell-selective single or combination therapy in 72 h of sample processing. The FUTC-based approach can also be used to predict in vivo resistance to known targeted therapies.For complete details on the use and execution of this protocol, please refer to Talwelkar et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-12T01:51:06Z |
| format | Article |
| id | doaj.art-006c6459adf0456781a8ab0e155bc7a4 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-12T01:51:06Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-006c6459adf0456781a8ab0e155bc7a42022-12-22T03:52:56ZengElsevierSTAR Protocols2666-16672022-12-0134101720Protocol to utilize fresh uncultured human lung tumor cells for personalized functional diagnosticsSarang S. Talwelkar0Iris A.K. Lähdeniemi1Mikko I. Mäyränpää2Annabrita Hemmes3Nora Linnavirta4Jari Räsänen5Aija Knuuttila6Krister Wennerberg7Emmy W. Verschuren8Institute for Molecular Medicine Finland (FIMM), HiLIFE, University of Helsinki, 00014 Helsinki, Finland; Institute of Biomedicine and MediCity Research Laboratories, University of Turku, Turku 20520, FinlandInstitute for Molecular Medicine Finland (FIMM), HiLIFE, University of Helsinki, 00014 Helsinki, FinlandDepartment of Pathology, University of Helsinki and Helsinki University Hospital, Helsinki, FinlandInstitute for Molecular Medicine Finland (FIMM), HiLIFE, University of Helsinki, 00014 Helsinki, FinlandInstitute for Molecular Medicine Finland (FIMM), HiLIFE, University of Helsinki, 00014 Helsinki, FinlandDepartment of Thoracic Surgery, Heart and Lung Center, Helsinki University Hospital, Helsinki, FinlandDepartment of Pulmonary Medicine, Heart and Lung Center and Cancer Center, Helsinki University Hospital, Helsinki, FinlandInstitute for Molecular Medicine Finland (FIMM), HiLIFE, University of Helsinki, 00014 Helsinki, Finland; BRIC - Biotech Research & Innovation Centre, University of Copenhagen, 2200 Copenhagen, Denmark; Corresponding authorInstitute for Molecular Medicine Finland (FIMM), HiLIFE, University of Helsinki, 00014 Helsinki, Finland; Corresponding authorSummary: Drug sensitivity data acquired from solid tumor-derived cultures are often unsuitable for personalized treatment guidance due to the lengthy turnaround time. Here, we present a protocol for determining ex vivo drug sensitivities using fresh uncultured human lung tumor-derived EpCAM+ epithelial cells (FUTCs). We describe steps for drug testing in FUTCs to identify tumor cell-selective single or combination therapy in 72 h of sample processing. The FUTC-based approach can also be used to predict in vivo resistance to known targeted therapies.For complete details on the use and execution of this protocol, please refer to Talwelkar et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722006001Cell biologyCell isolationCell-based assaysCancerHealth sciencesClinical protocol |
| spellingShingle | Sarang S. Talwelkar Iris A.K. Lähdeniemi Mikko I. Mäyränpää Annabrita Hemmes Nora Linnavirta Jari Räsänen Aija Knuuttila Krister Wennerberg Emmy W. Verschuren Protocol to utilize fresh uncultured human lung tumor cells for personalized functional diagnostics STAR Protocols Cell biology Cell isolation Cell-based assays Cancer Health sciences Clinical protocol |
| title | Protocol to utilize fresh uncultured human lung tumor cells for personalized functional diagnostics |
| title_full | Protocol to utilize fresh uncultured human lung tumor cells for personalized functional diagnostics |
| title_fullStr | Protocol to utilize fresh uncultured human lung tumor cells for personalized functional diagnostics |
| title_full_unstemmed | Protocol to utilize fresh uncultured human lung tumor cells for personalized functional diagnostics |
| title_short | Protocol to utilize fresh uncultured human lung tumor cells for personalized functional diagnostics |
| title_sort | protocol to utilize fresh uncultured human lung tumor cells for personalized functional diagnostics |
| topic | Cell biology Cell isolation Cell-based assays Cancer Health sciences Clinical protocol |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722006001 |
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