The Conserved Effector UvHrip1 Interacts with OsHGW and Infection of <i>Ustilaginoidea virens</i> Regulates Defense- and Heading Date-Related Signaling Pathway

The Conserved Effector UvHrip1 Interacts with OsHGW and Infection of <i>Ustilaginoidea virens</i> Regulates Defense- and Heading Date-Related Signaling Pathway

<i>Ustilaginoidea virens</i>, which causes rice false smut (RFS), is one of the most detrimental rice fungal diseases and poses a severe threat to rice production and quality. Effectors in <i>U. virens</i> often act as a group of essential virulence factors that play crucial...

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Bibliographic Details
Main Authors: Songhong Wei, Yingling Wang, Jianming Zhou, Shibo Xiang, Wenxian Sun, Xunwen Peng, Jing Li, Yingfan Hai, Yan Wang, Shuai Li
Format: Article
Language:English
Published: MDPI AG 2020-05-01
Series:International Journal of Molecular Sciences
Subjects:
effector
<i>Ustilaginoidea virens</i>
flowering time
innate immunity
pathogenicity
Online Access:https://www.mdpi.com/1422-0067/21/9/3376
Description
Summary:<i>Ustilaginoidea virens</i>, which causes rice false smut (RFS), is one of the most detrimental rice fungal diseases and poses a severe threat to rice production and quality. Effectors in <i>U. virens</i> often act as a group of essential virulence factors that play crucial roles in the interaction between host and the pathogen. Thus, the functions of individual effectors in <i>U. virens</i> need to be further explored. Here, we demonstrated a small secreted hypersensitive response-inducing protein (hrip), named UvHrip1, which was highly conserved in <i>U. virens</i> isolates. UvHrip1 was also proven to suppress necrosis-like defense symptoms in <i>N. benthamiana</i> induced by the oomycete elicitor INF1. The localization of UvHrip1 was mainly in the nuclei and cytoplasm via monitoring the UvHrip1-GFP fusion protein in rice cells. Furthermore, Y2H and BiFC assay demonstrated that UvHrip1 interacted with OsHGW, which is a critical regulator in heading date and grain weight signaling pathways in rice. Expression patterns of defense- and heading date-related genes, <i>OsPR1#051</i> and <i>OsMYB21</i>, were down-regulated over <i>U. virens</i> infection in rice. Collectively, our data provide a theory for gaining an insight into the molecular mechanisms underlying the UvHrip1 virulence function.
ISSN:1661-6596
1422-0067

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