Gamma-ray mutagenesis on grapevine rootstocks cultivated <em>in vitro</em> (1)
The efficiency of γ-irradiation as a tool for breeding grapevine rootstocks was investigated. One-bud cuttings from vitroplants of two varieties (Fercal and Gravesac) were subjected to gamma rays from Cs137 source at rates from 10 to 60 Grays (Gy) at intervals of 10. All the parameters observed were...
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| Format: | Article |
| Language: | English |
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International Viticulture and Enology Society
1995-03-01
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| Series: | OENO One |
| Subjects: | |
| Online Access: | https://oeno-one.eu/article/view/1717 |
| _version_ | 1818661837033439232 |
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| author | A. Lima da Silva J. P. Doazan |
| author_facet | A. Lima da Silva J. P. Doazan |
| author_sort | A. Lima da Silva |
| collection | DOAJ |
| description | The efficiency of γ-irradiation as a tool for breeding grapevine rootstocks was investigated. One-bud cuttings from vitroplants of two varieties (Fercal and Gravesac) were subjected to gamma rays from Cs137 source at rates from 10 to 60 Grays (Gy) at intervals of 10. All the parameters observed were affected at 20 Gy and upwards. Varietal susceptibility to irradiation was different since the maximum withstanding dose was 40 Gy for Gravesac and only 30 for Fercal. Besides, shoot development was more lowered than root development. The results showed that an increase of the irradiation dose decreased the rate of survival, the rhizogenesis and the growth of in vitro plants. On the V2 vegetative generation ofGravesac vitroplants changes in size, in leafform, in plant habits, in growth and chlorophyll deficient mutations were recorded. Moreover, after a 30 Gy irradiation variations of growth, dry weight, leaf area, number of stomata and photosynthesis rates were shown on the V3 generation. Accordingly this irradiation method is efficient to develop an in vitro variability. A sample of the surviving plants was established in the greenhouse for further screening.
(1) Communication faite au 6'®me Symposium International sur l'Amélioration de la Vigne, à Yalta (Crimée), Ukraine, du 4 au 10 septembre 1994 |
| first_indexed | 2024-12-17T04:51:24Z |
| format | Article |
| id | doaj.art-0071220d8f634d35bffc036db108ffd3 |
| institution | Directory Open Access Journal |
| issn | 2494-1271 |
| language | English |
| last_indexed | 2024-12-17T04:51:24Z |
| publishDate | 1995-03-01 |
| publisher | International Viticulture and Enology Society |
| record_format | Article |
| series | OENO One |
| spelling | doaj.art-0071220d8f634d35bffc036db108ffd32022-12-21T22:02:52ZengInternational Viticulture and Enology SocietyOENO One2494-12711995-03-012911910.20870/oeno-one.1995.29.1.17171717Gamma-ray mutagenesis on grapevine rootstocks cultivated <em>in vitro</em> (1)A. Lima da Silva0J. P. Doazan1Departamento de Fitotecnia, UFSC/PIBIO-CNPq, CP 476, 88040 Florianopolis, Santa Catarina (Brasil)INRA, Station de Recherches de Viticulture, B.P. 81, 33883 Villenave d'Ornon (France)The efficiency of γ-irradiation as a tool for breeding grapevine rootstocks was investigated. One-bud cuttings from vitroplants of two varieties (Fercal and Gravesac) were subjected to gamma rays from Cs137 source at rates from 10 to 60 Grays (Gy) at intervals of 10. All the parameters observed were affected at 20 Gy and upwards. Varietal susceptibility to irradiation was different since the maximum withstanding dose was 40 Gy for Gravesac and only 30 for Fercal. Besides, shoot development was more lowered than root development. The results showed that an increase of the irradiation dose decreased the rate of survival, the rhizogenesis and the growth of in vitro plants. On the V2 vegetative generation ofGravesac vitroplants changes in size, in leafform, in plant habits, in growth and chlorophyll deficient mutations were recorded. Moreover, after a 30 Gy irradiation variations of growth, dry weight, leaf area, number of stomata and photosynthesis rates were shown on the V3 generation. Accordingly this irradiation method is efficient to develop an in vitro variability. A sample of the surviving plants was established in the greenhouse for further screening. (1) Communication faite au 6'®me Symposium International sur l'Amélioration de la Vigne, à Yalta (Crimée), Ukraine, du 4 au 10 septembre 1994https://oeno-one.eu/article/view/1717irradiationrootstocksgrapevinein vitro culture |
| spellingShingle | A. Lima da Silva J. P. Doazan Gamma-ray mutagenesis on grapevine rootstocks cultivated <em>in vitro</em> (1) OENO One irradiation rootstocks grapevine in vitro culture |
| title | Gamma-ray mutagenesis on grapevine rootstocks cultivated <em>in vitro</em> (1) |
| title_full | Gamma-ray mutagenesis on grapevine rootstocks cultivated <em>in vitro</em> (1) |
| title_fullStr | Gamma-ray mutagenesis on grapevine rootstocks cultivated <em>in vitro</em> (1) |
| title_full_unstemmed | Gamma-ray mutagenesis on grapevine rootstocks cultivated <em>in vitro</em> (1) |
| title_short | Gamma-ray mutagenesis on grapevine rootstocks cultivated <em>in vitro</em> (1) |
| title_sort | gamma ray mutagenesis on grapevine rootstocks cultivated em in vitro em 1 |
| topic | irradiation rootstocks grapevine in vitro culture |
| url | https://oeno-one.eu/article/view/1717 |
| work_keys_str_mv | AT alimadasilva gammaraymutagenesisongrapevinerootstockscultivatedeminvitroem1 AT jpdoazan gammaraymutagenesisongrapevinerootstockscultivatedeminvitroem1 |