Evaluation of Toxicity and Efficacy of Inotodiol as an Anti-Inflammatory Agent Using Animal Model
Chaga mushroom (<i>Inonotus obliquus</i>) comprises polyphenolic compounds, triterpenoids, polysaccharides, and sterols. Among the triterpenoid components, inotodiol has been broadly examined because of its various biological activities. The purpose of this study is to examine inotodiol...
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MDPI AG
2022-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/15/4704 |
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| author | Thi Minh Nguyet Nguyen So-Young Ban Kyu-Been Park Chang-Kyu Lee Seoung-Woo Lee Young-Jin Lee Su-Min Baek Jin-Kyu Park My Tuyen Thi Nguyen Jaehan Kim Jihyun Park Jong-Tae Park |
| author_facet | Thi Minh Nguyet Nguyen So-Young Ban Kyu-Been Park Chang-Kyu Lee Seoung-Woo Lee Young-Jin Lee Su-Min Baek Jin-Kyu Park My Tuyen Thi Nguyen Jaehan Kim Jihyun Park Jong-Tae Park |
| author_sort | Thi Minh Nguyet Nguyen |
| collection | DOAJ |
| description | Chaga mushroom (<i>Inonotus obliquus</i>) comprises polyphenolic compounds, triterpenoids, polysaccharides, and sterols. Among the triterpenoid components, inotodiol has been broadly examined because of its various biological activities. The purpose of this study is to examine inotodiol from a safety point of view and to present the potential possibilities of inotodiol for medical usage. From chaga mushroom extract, crude inotodiol (INO20) and pure inotodiol (INO95) were produced. Mice were treated with either INO20 or INO95 once daily using oral administration for repeated dose toxicity evaluation. Serum biochemistry parameters were analyzed, and the level of pro-inflammatory cytokines in the serum was quantified. In parallel, the effect of inotodiol on food allergic symptoms was investigated. Repeated administration of inotodiol did not show any mortality or abnormalities in organs. In food allergy studies, the symptoms of diarrhea were ameliorated by administration with INO95 and INO20. Furthermore, the level of MCPT-1 decreased by treatment with inotodiol. In this study, we demonstrated for the first time that inotodiol does not cause any detrimental effect by showing anti-allergic activities in vivo by inhibiting mast cell function. Our data highlight the potential to use inotodiol as an immune modulator for diseases related to inflammation. |
| first_indexed | 2024-03-09T12:21:24Z |
| format | Article |
| id | doaj.art-0071a3bf918148a483b59e1151b7d549 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T12:21:24Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-0071a3bf918148a483b59e1151b7d5492023-11-30T22:39:30ZengMDPI AGMolecules1420-30492022-07-012715470410.3390/molecules27154704Evaluation of Toxicity and Efficacy of Inotodiol as an Anti-Inflammatory Agent Using Animal ModelThi Minh Nguyet Nguyen0So-Young Ban1Kyu-Been Park2Chang-Kyu Lee3Seoung-Woo Lee4Young-Jin Lee5Su-Min Baek6Jin-Kyu Park7My Tuyen Thi Nguyen8Jaehan Kim9Jihyun Park10Jong-Tae Park11CARBOEXPERT Inc., Daejeon 34134, KoreaCARBOEXPERT Inc., Daejeon 34134, KoreaCARBOEXPERT Inc., Daejeon 34134, KoreaCARBOEXPERT Inc., Daejeon 34134, KoreaDepartment of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, KoreaDepartment of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, KoreaDepartment of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, KoreaDepartment of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, KoreaDepartment of Food and Nutrition, Chungnam National University, Daejeon 34134, KoreaDepartment of Food and Nutrition, Chungnam National University, Daejeon 34134, KoreaCARBOEXPERT Inc., Daejeon 34134, KoreaCARBOEXPERT Inc., Daejeon 34134, KoreaChaga mushroom (<i>Inonotus obliquus</i>) comprises polyphenolic compounds, triterpenoids, polysaccharides, and sterols. Among the triterpenoid components, inotodiol has been broadly examined because of its various biological activities. The purpose of this study is to examine inotodiol from a safety point of view and to present the potential possibilities of inotodiol for medical usage. From chaga mushroom extract, crude inotodiol (INO20) and pure inotodiol (INO95) were produced. Mice were treated with either INO20 or INO95 once daily using oral administration for repeated dose toxicity evaluation. Serum biochemistry parameters were analyzed, and the level of pro-inflammatory cytokines in the serum was quantified. In parallel, the effect of inotodiol on food allergic symptoms was investigated. Repeated administration of inotodiol did not show any mortality or abnormalities in organs. In food allergy studies, the symptoms of diarrhea were ameliorated by administration with INO95 and INO20. Furthermore, the level of MCPT-1 decreased by treatment with inotodiol. In this study, we demonstrated for the first time that inotodiol does not cause any detrimental effect by showing anti-allergic activities in vivo by inhibiting mast cell function. Our data highlight the potential to use inotodiol as an immune modulator for diseases related to inflammation.https://www.mdpi.com/1420-3049/27/15/4704inotodioltoxicityinflammationallergymast cell |
| spellingShingle | Thi Minh Nguyet Nguyen So-Young Ban Kyu-Been Park Chang-Kyu Lee Seoung-Woo Lee Young-Jin Lee Su-Min Baek Jin-Kyu Park My Tuyen Thi Nguyen Jaehan Kim Jihyun Park Jong-Tae Park Evaluation of Toxicity and Efficacy of Inotodiol as an Anti-Inflammatory Agent Using Animal Model Molecules inotodiol toxicity inflammation allergy mast cell |
| title | Evaluation of Toxicity and Efficacy of Inotodiol as an Anti-Inflammatory Agent Using Animal Model |
| title_full | Evaluation of Toxicity and Efficacy of Inotodiol as an Anti-Inflammatory Agent Using Animal Model |
| title_fullStr | Evaluation of Toxicity and Efficacy of Inotodiol as an Anti-Inflammatory Agent Using Animal Model |
| title_full_unstemmed | Evaluation of Toxicity and Efficacy of Inotodiol as an Anti-Inflammatory Agent Using Animal Model |
| title_short | Evaluation of Toxicity and Efficacy of Inotodiol as an Anti-Inflammatory Agent Using Animal Model |
| title_sort | evaluation of toxicity and efficacy of inotodiol as an anti inflammatory agent using animal model |
| topic | inotodiol toxicity inflammation allergy mast cell |
| url | https://www.mdpi.com/1420-3049/27/15/4704 |
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