Tumor microenvironment characteristics and prognostic role of m6A modification in lung squamous cell carcinoma
Background: It has recently been determined that N6-methyladenosine (m6A) RNA methylation regulators have prominent effects on several cancers. However, the potential role of m6A modification in lung squamous cell carcinoma (LUSC) remains unclear. Methods: We evaluated the modification pattern of m6...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024028822 |
| _version_ | 1797259753984884736 |
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| author | Pei Li Peiyu Xiong Xinyun Li Xiaobo Zhang Xu Chen Wei Zhang Bo Jia Yu Lai |
| author_facet | Pei Li Peiyu Xiong Xinyun Li Xiaobo Zhang Xu Chen Wei Zhang Bo Jia Yu Lai |
| author_sort | Pei Li |
| collection | DOAJ |
| description | Background: It has recently been determined that N6-methyladenosine (m6A) RNA methylation regulators have prominent effects on several cancers. However, the potential role of m6A modification in lung squamous cell carcinoma (LUSC) remains unclear. Methods: We evaluated the modification pattern of m6A and studied the biological function of m6A regulators in LUSC. Then, we constructed the m6Ascore to predict the prognosis of LUSC and analyzed the relationship between the m6Ascore and tumor mutation burden, immune cell infiltration, and immunotherapy. Result: In the unsupervised consensus cluster analysis, three different m6Aclusters were identified, which correspond to an immune activation state, a moderate immune activation state, and an immune tolerance state. Forty-two genes related to the m6A phenotype were used to construct the m6Ascore; subsequently, multiple validations of the m6Ascore were carried out to determine the relationship between the score and immune cell infiltration and response to CTLA-4/PD-1 inhibitor treatment. Further analysis revealed that the m6Ascore could effectively predict the prognosis of LUSC and that the m6A phenotype-related genes, FAM162A and LOM4, might be potential biomarkers. Conclusion: These findings highlight the potential role of m6A modification in the prognosis, TME, and immunotherapy of LUSC and have profound implications for developing more effective personalized treatment strategies for LUSC. |
| first_indexed | 2024-03-07T18:35:49Z |
| format | Article |
| id | doaj.art-0071bfddad7f4ce096e990122af4cfef |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T23:14:27Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj.art-0071bfddad7f4ce096e990122af4cfef2024-03-17T07:56:29ZengElsevierHeliyon2405-84402024-03-01105e26851Tumor microenvironment characteristics and prognostic role of m6A modification in lung squamous cell carcinomaPei Li0Peiyu Xiong1Xinyun Li2Xiaobo Zhang3Xu Chen4Wei Zhang5Bo Jia6Yu Lai7School of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, ChinaSchool of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, ChinaSichuan College of Traditional Chinese Medicine, Mianyang, 621000, ChinaSchool of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, ChinaSchool of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, ChinaSchool of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, ChinaSchool of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, ChinaSchool of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China; Corresponding author. No.1166 Liutai Avenue, Chengdu, 611137, China.Background: It has recently been determined that N6-methyladenosine (m6A) RNA methylation regulators have prominent effects on several cancers. However, the potential role of m6A modification in lung squamous cell carcinoma (LUSC) remains unclear. Methods: We evaluated the modification pattern of m6A and studied the biological function of m6A regulators in LUSC. Then, we constructed the m6Ascore to predict the prognosis of LUSC and analyzed the relationship between the m6Ascore and tumor mutation burden, immune cell infiltration, and immunotherapy. Result: In the unsupervised consensus cluster analysis, three different m6Aclusters were identified, which correspond to an immune activation state, a moderate immune activation state, and an immune tolerance state. Forty-two genes related to the m6A phenotype were used to construct the m6Ascore; subsequently, multiple validations of the m6Ascore were carried out to determine the relationship between the score and immune cell infiltration and response to CTLA-4/PD-1 inhibitor treatment. Further analysis revealed that the m6Ascore could effectively predict the prognosis of LUSC and that the m6A phenotype-related genes, FAM162A and LOM4, might be potential biomarkers. Conclusion: These findings highlight the potential role of m6A modification in the prognosis, TME, and immunotherapy of LUSC and have profound implications for developing more effective personalized treatment strategies for LUSC.http://www.sciencedirect.com/science/article/pii/S2405844024028822Lung squamous cell carcinomam6APrognosisTumor microenvironmentImmunotherapy |
| spellingShingle | Pei Li Peiyu Xiong Xinyun Li Xiaobo Zhang Xu Chen Wei Zhang Bo Jia Yu Lai Tumor microenvironment characteristics and prognostic role of m6A modification in lung squamous cell carcinoma Heliyon Lung squamous cell carcinoma m6A Prognosis Tumor microenvironment Immunotherapy |
| title | Tumor microenvironment characteristics and prognostic role of m6A modification in lung squamous cell carcinoma |
| title_full | Tumor microenvironment characteristics and prognostic role of m6A modification in lung squamous cell carcinoma |
| title_fullStr | Tumor microenvironment characteristics and prognostic role of m6A modification in lung squamous cell carcinoma |
| title_full_unstemmed | Tumor microenvironment characteristics and prognostic role of m6A modification in lung squamous cell carcinoma |
| title_short | Tumor microenvironment characteristics and prognostic role of m6A modification in lung squamous cell carcinoma |
| title_sort | tumor microenvironment characteristics and prognostic role of m6a modification in lung squamous cell carcinoma |
| topic | Lung squamous cell carcinoma m6A Prognosis Tumor microenvironment Immunotherapy |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024028822 |
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