Characterization of the biosynthetic gene cluster for cryptic phthoxazolin A in Streptomyces avermitilis.
Phthoxazolin A, an oxazole-containing polyketide, has a broad spectrum of anti-oomycete activity and herbicidal activity. We recently identified phthoxazolin A as a cryptic metabolite of Streptomyces avermitilis that produces the important anthelmintic agent avermectin. Even though genome data of S....
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5764310?pdf=render |
| _version_ | 1819108402376212480 |
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| author | Dian Anggraini Suroto Shigeru Kitani Masayoshi Arai Haruo Ikeda Takuya Nihira |
| author_facet | Dian Anggraini Suroto Shigeru Kitani Masayoshi Arai Haruo Ikeda Takuya Nihira |
| author_sort | Dian Anggraini Suroto |
| collection | DOAJ |
| description | Phthoxazolin A, an oxazole-containing polyketide, has a broad spectrum of anti-oomycete activity and herbicidal activity. We recently identified phthoxazolin A as a cryptic metabolite of Streptomyces avermitilis that produces the important anthelmintic agent avermectin. Even though genome data of S. avermitilis is publicly available, no plausible biosynthetic gene cluster for phthoxazolin A is apparent in the sequence data. Here, we identified and characterized the phthoxazolin A (ptx) biosynthetic gene cluster through genome sequencing, comparative genomic analysis, and gene disruption. Sequence analysis uncovered that the putative ptx biosynthetic genes are laid on an extra genomic region that is not found in the public database, and 8 open reading frames in the extra genomic region could be assigned roles in the biosynthesis of the oxazole ring, triene polyketide and carbamoyl moieties. Disruption of the ptxA gene encoding a discrete acyltransferase resulted in a complete loss of phthoxazolin A production, confirming that the trans-AT type I PKS system is responsible for the phthoxazolin A biosynthesis. Based on the predicted functional domains in the ptx assembly line, we propose the biosynthetic pathway of phthoxazolin A. |
| first_indexed | 2024-12-22T03:09:22Z |
| format | Article |
| id | doaj.art-0074bde16a5849838ecf18038825ed0c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T03:09:22Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0074bde16a5849838ecf18038825ed0c2022-12-21T18:40:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01131e019097310.1371/journal.pone.0190973Characterization of the biosynthetic gene cluster for cryptic phthoxazolin A in Streptomyces avermitilis.Dian Anggraini SurotoShigeru KitaniMasayoshi AraiHaruo IkedaTakuya NihiraPhthoxazolin A, an oxazole-containing polyketide, has a broad spectrum of anti-oomycete activity and herbicidal activity. We recently identified phthoxazolin A as a cryptic metabolite of Streptomyces avermitilis that produces the important anthelmintic agent avermectin. Even though genome data of S. avermitilis is publicly available, no plausible biosynthetic gene cluster for phthoxazolin A is apparent in the sequence data. Here, we identified and characterized the phthoxazolin A (ptx) biosynthetic gene cluster through genome sequencing, comparative genomic analysis, and gene disruption. Sequence analysis uncovered that the putative ptx biosynthetic genes are laid on an extra genomic region that is not found in the public database, and 8 open reading frames in the extra genomic region could be assigned roles in the biosynthesis of the oxazole ring, triene polyketide and carbamoyl moieties. Disruption of the ptxA gene encoding a discrete acyltransferase resulted in a complete loss of phthoxazolin A production, confirming that the trans-AT type I PKS system is responsible for the phthoxazolin A biosynthesis. Based on the predicted functional domains in the ptx assembly line, we propose the biosynthetic pathway of phthoxazolin A.http://europepmc.org/articles/PMC5764310?pdf=render |
| spellingShingle | Dian Anggraini Suroto Shigeru Kitani Masayoshi Arai Haruo Ikeda Takuya Nihira Characterization of the biosynthetic gene cluster for cryptic phthoxazolin A in Streptomyces avermitilis. PLoS ONE |
| title | Characterization of the biosynthetic gene cluster for cryptic phthoxazolin A in Streptomyces avermitilis. |
| title_full | Characterization of the biosynthetic gene cluster for cryptic phthoxazolin A in Streptomyces avermitilis. |
| title_fullStr | Characterization of the biosynthetic gene cluster for cryptic phthoxazolin A in Streptomyces avermitilis. |
| title_full_unstemmed | Characterization of the biosynthetic gene cluster for cryptic phthoxazolin A in Streptomyces avermitilis. |
| title_short | Characterization of the biosynthetic gene cluster for cryptic phthoxazolin A in Streptomyces avermitilis. |
| title_sort | characterization of the biosynthetic gene cluster for cryptic phthoxazolin a in streptomyces avermitilis |
| url | http://europepmc.org/articles/PMC5764310?pdf=render |
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