rRNA methylation by Spb1 regulates the GTPase activity of Nog2 during 60S ribosomal subunit assembly
Regulation of 60S biogenesis remains poorly understood. Using cryo-EM, the authors show that failure of Spb1 to methylate the A-loop nucleotide G2922 prematurely activates the GTPase Nog2, suggesting that Spb1 and Nog2 form a kinetic checkpoint during ribosome maturation.
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-36867-5 |
| _version_ | 1797864192856817664 |
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| author | Kamil Sekulski Victor Emmanuel Cruz Christine S. Weirich Jan P. Erzberger |
| author_facet | Kamil Sekulski Victor Emmanuel Cruz Christine S. Weirich Jan P. Erzberger |
| author_sort | Kamil Sekulski |
| collection | DOAJ |
| description | Regulation of 60S biogenesis remains poorly understood. Using cryo-EM, the authors show that failure of Spb1 to methylate the A-loop nucleotide G2922 prematurely activates the GTPase Nog2, suggesting that Spb1 and Nog2 form a kinetic checkpoint during ribosome maturation. |
| first_indexed | 2024-04-09T22:47:36Z |
| format | Article |
| id | doaj.art-0079a91e9ebe4720b71e0b1add6eb261 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-09T22:47:36Z |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-0079a91e9ebe4720b71e0b1add6eb2612023-03-22T11:43:47ZengNature PortfolioNature Communications2041-17232023-03-0114111110.1038/s41467-023-36867-5rRNA methylation by Spb1 regulates the GTPase activity of Nog2 during 60S ribosomal subunit assemblyKamil Sekulski0Victor Emmanuel Cruz1Christine S. Weirich2Jan P. Erzberger3Department of Biophysics, UT Southwestern Medical CenterDepartment of Biophysics, UT Southwestern Medical CenterDepartment of Biophysics, UT Southwestern Medical CenterDepartment of Biophysics, UT Southwestern Medical CenterRegulation of 60S biogenesis remains poorly understood. Using cryo-EM, the authors show that failure of Spb1 to methylate the A-loop nucleotide G2922 prematurely activates the GTPase Nog2, suggesting that Spb1 and Nog2 form a kinetic checkpoint during ribosome maturation.https://doi.org/10.1038/s41467-023-36867-5 |
| spellingShingle | Kamil Sekulski Victor Emmanuel Cruz Christine S. Weirich Jan P. Erzberger rRNA methylation by Spb1 regulates the GTPase activity of Nog2 during 60S ribosomal subunit assembly Nature Communications |
| title | rRNA methylation by Spb1 regulates the GTPase activity of Nog2 during 60S ribosomal subunit assembly |
| title_full | rRNA methylation by Spb1 regulates the GTPase activity of Nog2 during 60S ribosomal subunit assembly |
| title_fullStr | rRNA methylation by Spb1 regulates the GTPase activity of Nog2 during 60S ribosomal subunit assembly |
| title_full_unstemmed | rRNA methylation by Spb1 regulates the GTPase activity of Nog2 during 60S ribosomal subunit assembly |
| title_short | rRNA methylation by Spb1 regulates the GTPase activity of Nog2 during 60S ribosomal subunit assembly |
| title_sort | rrna methylation by spb1 regulates the gtpase activity of nog2 during 60s ribosomal subunit assembly |
| url | https://doi.org/10.1038/s41467-023-36867-5 |
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