Remodeling of Endothelial Cell Nucleus Exposed to Three Different Mechanical Stimuli
Cell Nuclei play a critical role in controlling gene expression and replicating DNA, and is known to deform in association with cell shape changes in response to external forces. This study dealed with morphological analysis to quantitatively assess the effect of three different mechanical stimuli i...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The Japan Society of Mechanical Engineers
2008-04-01
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| Series: | Journal of Biomechanical Science and Engineering |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jbse/3/2/3_2_63/_pdf/-char/en |
| _version_ | 1818532547953426432 |
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| author | Toshiro OHASHI Kazuhiko HANAMURA Daisaku AZUMA Naoya SAKAMOTO Masaaki SATO |
| author_facet | Toshiro OHASHI Kazuhiko HANAMURA Daisaku AZUMA Naoya SAKAMOTO Masaaki SATO |
| author_sort | Toshiro OHASHI |
| collection | DOAJ |
| description | Cell Nuclei play a critical role in controlling gene expression and replicating DNA, and is known to deform in association with cell shape changes in response to external forces. This study dealed with morphological analysis to quantitatively assess the effect of three different mechanical stimuli including fluid shear stress, cyclic stretching, and hydrostatic pressure on nucleus morphology. Fluorescence images showed that fluid shear stress and cyclic stretching induced cell elongation and orientation very specifically to the direction of flow and stretch, respectively. In contrast, hydrostatic pressure induced cell elongation at non-preferred orientation. The nuclei were also found to deform in the same manner as that of the cells, which was, in particular, dependent on the type of mechanical stimuli, possibly suggesting the direct mechanical linkages between cell surface receptors, cytoskeletal meshworks, and nuclei. It was also shown that cytoskeletal meshworks may contribute to pre-existing strain of the nuclei. |
| first_indexed | 2024-12-11T17:46:57Z |
| format | Article |
| id | doaj.art-007b7e5a03434c5aa885cc9c3c9f3bb0 |
| institution | Directory Open Access Journal |
| issn | 1880-9863 |
| language | English |
| last_indexed | 2024-12-11T17:46:57Z |
| publishDate | 2008-04-01 |
| publisher | The Japan Society of Mechanical Engineers |
| record_format | Article |
| series | Journal of Biomechanical Science and Engineering |
| spelling | doaj.art-007b7e5a03434c5aa885cc9c3c9f3bb02022-12-22T00:56:20ZengThe Japan Society of Mechanical EngineersJournal of Biomechanical Science and Engineering1880-98632008-04-0132637410.1299/jbse.3.63jbseRemodeling of Endothelial Cell Nucleus Exposed to Three Different Mechanical StimuliToshiro OHASHI0Kazuhiko HANAMURA1Daisaku AZUMA2Naoya SAKAMOTO3Masaaki SATO4Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku UniversityDepartment of Bioengineering and Robotics, Graduate School of Engineering, Tohoku UniversityDepartment of Bioengineering and Robotics, Graduate School of Engineering, Tohoku UniversityDepartment of Bioengineering and Robotics, Graduate School of Engineering, Tohoku UniversityDepartment of Bioengineering and Robotics, Graduate School of Engineering, Tohoku UniversityCell Nuclei play a critical role in controlling gene expression and replicating DNA, and is known to deform in association with cell shape changes in response to external forces. This study dealed with morphological analysis to quantitatively assess the effect of three different mechanical stimuli including fluid shear stress, cyclic stretching, and hydrostatic pressure on nucleus morphology. Fluorescence images showed that fluid shear stress and cyclic stretching induced cell elongation and orientation very specifically to the direction of flow and stretch, respectively. In contrast, hydrostatic pressure induced cell elongation at non-preferred orientation. The nuclei were also found to deform in the same manner as that of the cells, which was, in particular, dependent on the type of mechanical stimuli, possibly suggesting the direct mechanical linkages between cell surface receptors, cytoskeletal meshworks, and nuclei. It was also shown that cytoskeletal meshworks may contribute to pre-existing strain of the nuclei.https://www.jstage.jst.go.jp/article/jbse/3/2/3_2_63/_pdf/-char/enendothelial cellsnucleus remodelingshear stresscyclic stretchinghydrostatic pressurecytoskeletal meshworks |
| spellingShingle | Toshiro OHASHI Kazuhiko HANAMURA Daisaku AZUMA Naoya SAKAMOTO Masaaki SATO Remodeling of Endothelial Cell Nucleus Exposed to Three Different Mechanical Stimuli Journal of Biomechanical Science and Engineering endothelial cells nucleus remodeling shear stress cyclic stretching hydrostatic pressure cytoskeletal meshworks |
| title | Remodeling of Endothelial Cell Nucleus Exposed to Three Different Mechanical Stimuli |
| title_full | Remodeling of Endothelial Cell Nucleus Exposed to Three Different Mechanical Stimuli |
| title_fullStr | Remodeling of Endothelial Cell Nucleus Exposed to Three Different Mechanical Stimuli |
| title_full_unstemmed | Remodeling of Endothelial Cell Nucleus Exposed to Three Different Mechanical Stimuli |
| title_short | Remodeling of Endothelial Cell Nucleus Exposed to Three Different Mechanical Stimuli |
| title_sort | remodeling of endothelial cell nucleus exposed to three different mechanical stimuli |
| topic | endothelial cells nucleus remodeling shear stress cyclic stretching hydrostatic pressure cytoskeletal meshworks |
| url | https://www.jstage.jst.go.jp/article/jbse/3/2/3_2_63/_pdf/-char/en |
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