Prevalence and Molecular Characteristics of Avian-Origin mcr-1-Harboring Escherichia coli in Shandong Province, China
The objective of this study was to evaluate the prevalence and characteristics of avian-origin mcr-1-harbouring Escherichia coli in Shandong Province, China. During 2017—2018, a total of 668 non-duplicate E. coli isolates were separately collected from 8eight large intensive poultry farms in Shandon...
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Frontiers Media S.A.
2020-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.00255/full |
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| author | Xiaonan Zhao Zhengjie Liu Yin Zhang Xiaomeng Yuan Xiaomeng Yuan Ming Hu Yuqing Liu |
| author_facet | Xiaonan Zhao Zhengjie Liu Yin Zhang Xiaomeng Yuan Xiaomeng Yuan Ming Hu Yuqing Liu |
| author_sort | Xiaonan Zhao |
| collection | DOAJ |
| description | The objective of this study was to evaluate the prevalence and characteristics of avian-origin mcr-1-harbouring Escherichia coli in Shandong Province, China. During 2017—2018, a total of 668 non-duplicate E. coli isolates were separately collected from 8eight large intensive poultry farms in Shandong Province. Antimicrobial susceptibility testing for 10 antimicrobial agents commonly used in farms was performed on all E. coli isolates by the agar dilution method; the mobile colistin resistance gene (mcr-1) gene was screened by PCR, and mcr-1 positive isolates were PCR-screened for antimicrobial resistance genes and typed by multi-locus sequence typing (MLST). Among the 668 E. coli, 102 (15.3%) harbored the mcr-1 gene; high antimicrobial resistance rates were observed for ampicillin (100/102, 98.0%), followed by amoxicillin (99/102, 97.1%) and florfenicol (97/102, 95.1%), and a low level of resistance was found for amoxicillin/clavulanic acid (24/102, 23.5%). Five ESBL genes were detected, and all isolates carried blaTEM (102/102, 100%), followed by blaCTX–M (90/102, 88.2%). Four PMQR genes were detected; aac(6)-Ib-cr (40/102, 39.2%) was the most commonly isolated PMQR gene, followed by qnrA (10/102, 9.8%). Thirty-eight different kinds of STs were identified, and the dominant ST was ST93 (19/102, 18.6%), followed by ST48 (9/102, 8.8%). In summary, E. coli from poultry in Shandong could be a reservoir for the mcr-1 gene, which could pose serious risks to human public health. |
| first_indexed | 2024-12-11T15:48:28Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T15:48:28Z |
| publishDate | 2020-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-007bcbd8302a42dcb62261ceb86439ea2022-12-22T00:59:38ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-02-011110.3389/fmicb.2020.00255507283Prevalence and Molecular Characteristics of Avian-Origin mcr-1-Harboring Escherichia coli in Shandong Province, ChinaXiaonan Zhao0Zhengjie Liu1Yin Zhang2Xiaomeng Yuan3Xiaomeng Yuan4Ming Hu5Yuqing Liu6Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Shandong, ChinaInstitute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Shandong, ChinaInstitute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Shandong, ChinaInstitute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Shandong, ChinaCollege of Life Sciences, Shandong Normal University, Shandong, ChinaInstitute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Shandong, ChinaInstitute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Shandong, ChinaThe objective of this study was to evaluate the prevalence and characteristics of avian-origin mcr-1-harbouring Escherichia coli in Shandong Province, China. During 2017—2018, a total of 668 non-duplicate E. coli isolates were separately collected from 8eight large intensive poultry farms in Shandong Province. Antimicrobial susceptibility testing for 10 antimicrobial agents commonly used in farms was performed on all E. coli isolates by the agar dilution method; the mobile colistin resistance gene (mcr-1) gene was screened by PCR, and mcr-1 positive isolates were PCR-screened for antimicrobial resistance genes and typed by multi-locus sequence typing (MLST). Among the 668 E. coli, 102 (15.3%) harbored the mcr-1 gene; high antimicrobial resistance rates were observed for ampicillin (100/102, 98.0%), followed by amoxicillin (99/102, 97.1%) and florfenicol (97/102, 95.1%), and a low level of resistance was found for amoxicillin/clavulanic acid (24/102, 23.5%). Five ESBL genes were detected, and all isolates carried blaTEM (102/102, 100%), followed by blaCTX–M (90/102, 88.2%). Four PMQR genes were detected; aac(6)-Ib-cr (40/102, 39.2%) was the most commonly isolated PMQR gene, followed by qnrA (10/102, 9.8%). Thirty-eight different kinds of STs were identified, and the dominant ST was ST93 (19/102, 18.6%), followed by ST48 (9/102, 8.8%). In summary, E. coli from poultry in Shandong could be a reservoir for the mcr-1 gene, which could pose serious risks to human public health.https://www.frontiersin.org/article/10.3389/fmicb.2020.00255/fullEscherichia colimcr-1poultryresistanceMLST |
| spellingShingle | Xiaonan Zhao Zhengjie Liu Yin Zhang Xiaomeng Yuan Xiaomeng Yuan Ming Hu Yuqing Liu Prevalence and Molecular Characteristics of Avian-Origin mcr-1-Harboring Escherichia coli in Shandong Province, China Frontiers in Microbiology Escherichia coli mcr-1 poultry resistance MLST |
| title | Prevalence and Molecular Characteristics of Avian-Origin mcr-1-Harboring Escherichia coli in Shandong Province, China |
| title_full | Prevalence and Molecular Characteristics of Avian-Origin mcr-1-Harboring Escherichia coli in Shandong Province, China |
| title_fullStr | Prevalence and Molecular Characteristics of Avian-Origin mcr-1-Harboring Escherichia coli in Shandong Province, China |
| title_full_unstemmed | Prevalence and Molecular Characteristics of Avian-Origin mcr-1-Harboring Escherichia coli in Shandong Province, China |
| title_short | Prevalence and Molecular Characteristics of Avian-Origin mcr-1-Harboring Escherichia coli in Shandong Province, China |
| title_sort | prevalence and molecular characteristics of avian origin mcr 1 harboring escherichia coli in shandong province china |
| topic | Escherichia coli mcr-1 poultry resistance MLST |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.00255/full |
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