Allosteric Modulation of the CB1 Cannabinoid Receptor by Cannabidiol—A Molecular Modeling Study of the N-Terminal Domain and the Allosteric-Orthosteric Coupling
The CB<sub>1</sub> cannabinoid receptor (CB<sub>1</sub>R) contains one of the longest N termini among class A G protein-coupled receptors. Mutagenesis studies suggest that the allosteric binding site of cannabidiol (CBD) involves residues from the N terminal domain. In order...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/9/2456 |
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| author | Jakub Jakowiecki Renata Abel Urszula Orzeł Paweł Pasznik Robert Preissner Sławomir Filipek |
| author_facet | Jakub Jakowiecki Renata Abel Urszula Orzeł Paweł Pasznik Robert Preissner Sławomir Filipek |
| author_sort | Jakub Jakowiecki |
| collection | DOAJ |
| description | The CB<sub>1</sub> cannabinoid receptor (CB<sub>1</sub>R) contains one of the longest N termini among class A G protein-coupled receptors. Mutagenesis studies suggest that the allosteric binding site of cannabidiol (CBD) involves residues from the N terminal domain. In order to study the allosteric binding of CBD to CB<sub>1</sub>R we modeled the whole N-terminus of this receptor using the replica exchange molecular dynamics with solute tempering (REST2) approach. Then, the obtained structures of CB<sub>1</sub>R with the N terminus were used for ligand docking. A natural cannabinoid receptor agonist, Δ<sup>9</sup>-THC, was docked to the orthosteric site and a negative allosteric modulator, CBD, to the allosteric site positioned between extracellular ends of helices TM1 and TM2. The molecular dynamics simulations were then performed for CB<sub>1</sub>R with ligands: (i) CBD together with THC, and (ii) THC-only. Analyses of the differences in the residue-residue interaction patterns between those two cases allowed us to elucidate the allosteric network responsible for the modulation of the CB<sub>1</sub>R by CBD. In addition, we identified the changes in the orthosteric binding mode of Δ<sup>9</sup>-THC, as well as the changes in its binding energy, caused by the CBD allosteric binding. We have also found that the presence of a complete N-terminal domain is essential for a stable binding of CBD in the allosteric site of CB<sub>1</sub>R as well as for the allosteric-orthosteric coupling mechanism. |
| first_indexed | 2024-03-10T12:02:25Z |
| format | Article |
| id | doaj.art-007d1f4d20d541678928de7c3bdebe39 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T12:02:25Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-007d1f4d20d541678928de7c3bdebe392023-11-21T16:47:19ZengMDPI AGMolecules1420-30492021-04-01269245610.3390/molecules26092456Allosteric Modulation of the CB1 Cannabinoid Receptor by Cannabidiol—A Molecular Modeling Study of the N-Terminal Domain and the Allosteric-Orthosteric CouplingJakub Jakowiecki0Renata Abel1Urszula Orzeł2Paweł Pasznik3Robert Preissner4Sławomir Filipek5Biological and Chemical Research Centre, Faculty of Chemistry, University of Warsaw, 02093 Warsaw, PolandStructural Bioinformatics Group, Institute for Physiology, Charité–University Medicine Berlin, 10115 Berlin, GermanyBiological and Chemical Research Centre, Faculty of Chemistry, University of Warsaw, 02093 Warsaw, PolandBiological and Chemical Research Centre, Faculty of Chemistry, University of Warsaw, 02093 Warsaw, PolandStructural Bioinformatics Group, Institute for Physiology, Charité–University Medicine Berlin, 10115 Berlin, GermanyBiological and Chemical Research Centre, Faculty of Chemistry, University of Warsaw, 02093 Warsaw, PolandThe CB<sub>1</sub> cannabinoid receptor (CB<sub>1</sub>R) contains one of the longest N termini among class A G protein-coupled receptors. Mutagenesis studies suggest that the allosteric binding site of cannabidiol (CBD) involves residues from the N terminal domain. In order to study the allosteric binding of CBD to CB<sub>1</sub>R we modeled the whole N-terminus of this receptor using the replica exchange molecular dynamics with solute tempering (REST2) approach. Then, the obtained structures of CB<sub>1</sub>R with the N terminus were used for ligand docking. A natural cannabinoid receptor agonist, Δ<sup>9</sup>-THC, was docked to the orthosteric site and a negative allosteric modulator, CBD, to the allosteric site positioned between extracellular ends of helices TM1 and TM2. The molecular dynamics simulations were then performed for CB<sub>1</sub>R with ligands: (i) CBD together with THC, and (ii) THC-only. Analyses of the differences in the residue-residue interaction patterns between those two cases allowed us to elucidate the allosteric network responsible for the modulation of the CB<sub>1</sub>R by CBD. In addition, we identified the changes in the orthosteric binding mode of Δ<sup>9</sup>-THC, as well as the changes in its binding energy, caused by the CBD allosteric binding. We have also found that the presence of a complete N-terminal domain is essential for a stable binding of CBD in the allosteric site of CB<sub>1</sub>R as well as for the allosteric-orthosteric coupling mechanism.https://www.mdpi.com/1420-3049/26/9/2456CB1cannabinoid receptorGPCRsallosteric modulationCBDTHC |
| spellingShingle | Jakub Jakowiecki Renata Abel Urszula Orzeł Paweł Pasznik Robert Preissner Sławomir Filipek Allosteric Modulation of the CB1 Cannabinoid Receptor by Cannabidiol—A Molecular Modeling Study of the N-Terminal Domain and the Allosteric-Orthosteric Coupling Molecules CB1 cannabinoid receptor GPCRs allosteric modulation CBD THC |
| title | Allosteric Modulation of the CB1 Cannabinoid Receptor by Cannabidiol—A Molecular Modeling Study of the N-Terminal Domain and the Allosteric-Orthosteric Coupling |
| title_full | Allosteric Modulation of the CB1 Cannabinoid Receptor by Cannabidiol—A Molecular Modeling Study of the N-Terminal Domain and the Allosteric-Orthosteric Coupling |
| title_fullStr | Allosteric Modulation of the CB1 Cannabinoid Receptor by Cannabidiol—A Molecular Modeling Study of the N-Terminal Domain and the Allosteric-Orthosteric Coupling |
| title_full_unstemmed | Allosteric Modulation of the CB1 Cannabinoid Receptor by Cannabidiol—A Molecular Modeling Study of the N-Terminal Domain and the Allosteric-Orthosteric Coupling |
| title_short | Allosteric Modulation of the CB1 Cannabinoid Receptor by Cannabidiol—A Molecular Modeling Study of the N-Terminal Domain and the Allosteric-Orthosteric Coupling |
| title_sort | allosteric modulation of the cb1 cannabinoid receptor by cannabidiol a molecular modeling study of the n terminal domain and the allosteric orthosteric coupling |
| topic | CB1 cannabinoid receptor GPCRs allosteric modulation CBD THC |
| url | https://www.mdpi.com/1420-3049/26/9/2456 |
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