Anti-Helicobacter pylori activity of crude N-acetylneuraminic acid isolated from glycomacropeptide of whey
Abstract Helicobacter pylori colonizes the gastric mucosa of about half of the world’s population, causing chronic gastritis and gastric cancer. An increasing emergence of antibiotic-resistant H. pylori arouses demand on alternative non-antibiotic-based therapies. In this study, we freshly prepared...
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| Format: | Article |
| Language: | English |
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BMC
2016-12-01
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| Series: | Laboratory Animal Research |
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| Online Access: | http://link.springer.com/article/10.5625/lar.2016.32.2.99 |
| _version_ | 1818159732322467840 |
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| author | Dong-Jae Kim Min-Jung Kang Jin-A Choi Dae-Seung Na Jin-Beom Kim Chun-Soo Na Jong-Hwan Park |
| author_facet | Dong-Jae Kim Min-Jung Kang Jin-A Choi Dae-Seung Na Jin-Beom Kim Chun-Soo Na Jong-Hwan Park |
| author_sort | Dong-Jae Kim |
| collection | DOAJ |
| description | Abstract Helicobacter pylori colonizes the gastric mucosa of about half of the world’s population, causing chronic gastritis and gastric cancer. An increasing emergence of antibiotic-resistant H. pylori arouses demand on alternative non-antibiotic-based therapies. In this study, we freshly prepared crude N-acetylneuraminic acid obtained from glycomacropeptide (G-NANA) of whey through a neuraminidase-mediated reaction and evaluated its antibacterial ability against H. pylori and H. felis. Overnight cultures of the H. pylori were diluted with fresh media and different concentrations (1-150 mg/mL) of crude G-NANA were added directly to the culture tube. Bacterial growth was evaluated by measuring the optical density of the culture medium and the number of viable bacteria was determined by a direct count of the colony forming units (CFU) on agar plates. For the in vivo study, mice were orally infected with 100 μL (5×108 cfu/mL) of H. felis four times at a day’s interval, accompanied by a daily administration of crude G-NANA or vehicle. A day after the last infection, the mice were daily administered the crude G-NANA (0, 75, and 300 mg/mL) for 10 days and euthanized. Their stomachs were collected and bacterial colonization was determined by quantitative real-time PCR. Crude G-NANA inhibited H. pylori’s growth and reduced the number of viable bacteria in a dose-dependent manner. Furthermore, crude G-NANA inhibited bacterial colonization in the mice. These results showed that crude G-NANA has antibacterial activity against Helicobacter and demonstrated its therapeutic potential for the prevention of chronic gastritis and gastric carcinogenesis induced by Helicobacter infection in humans. |
| first_indexed | 2024-12-11T15:50:39Z |
| format | Article |
| id | doaj.art-007dd573a7ce4760b0ffd58301f05604 |
| institution | Directory Open Access Journal |
| issn | 2233-7660 |
| language | English |
| last_indexed | 2024-12-11T15:50:39Z |
| publishDate | 2016-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Laboratory Animal Research |
| spelling | doaj.art-007dd573a7ce4760b0ffd58301f056042022-12-22T00:59:35ZengBMCLaboratory Animal Research2233-76602016-12-013229910410.5625/lar.2016.32.2.99Anti-Helicobacter pylori activity of crude N-acetylneuraminic acid isolated from glycomacropeptide of wheyDong-Jae Kim0Min-Jung Kang1Jin-A Choi2Dae-Seung Na3Jin-Beom Kim4Chun-Soo Na5Jong-Hwan Park6Laboratory Animal Resource Center, Daegu Gyeongbuk Institute of Science & Technology (DGISTLaboratory Animal Medicine and Brain Korea 21 PLUS Project, College of Veterinary Medicine, Chonnam National UniversityDepartment of Biochemistry, College of Medicine, Konyang UniversityLifetree Biotechnology Institute, Lifetree Bitotech Co. Ltd.Lifetree Biotechnology Institute, Lifetree Bitotech Co. Ltd.Lifetree Biotechnology Institute, Lifetree Bitotech Co. Ltd.Laboratory Animal Medicine and Brain Korea 21 PLUS Project, College of Veterinary Medicine, Chonnam National UniversityAbstract Helicobacter pylori colonizes the gastric mucosa of about half of the world’s population, causing chronic gastritis and gastric cancer. An increasing emergence of antibiotic-resistant H. pylori arouses demand on alternative non-antibiotic-based therapies. In this study, we freshly prepared crude N-acetylneuraminic acid obtained from glycomacropeptide (G-NANA) of whey through a neuraminidase-mediated reaction and evaluated its antibacterial ability against H. pylori and H. felis. Overnight cultures of the H. pylori were diluted with fresh media and different concentrations (1-150 mg/mL) of crude G-NANA were added directly to the culture tube. Bacterial growth was evaluated by measuring the optical density of the culture medium and the number of viable bacteria was determined by a direct count of the colony forming units (CFU) on agar plates. For the in vivo study, mice were orally infected with 100 μL (5×108 cfu/mL) of H. felis four times at a day’s interval, accompanied by a daily administration of crude G-NANA or vehicle. A day after the last infection, the mice were daily administered the crude G-NANA (0, 75, and 300 mg/mL) for 10 days and euthanized. Their stomachs were collected and bacterial colonization was determined by quantitative real-time PCR. Crude G-NANA inhibited H. pylori’s growth and reduced the number of viable bacteria in a dose-dependent manner. Furthermore, crude G-NANA inhibited bacterial colonization in the mice. These results showed that crude G-NANA has antibacterial activity against Helicobacter and demonstrated its therapeutic potential for the prevention of chronic gastritis and gastric carcinogenesis induced by Helicobacter infection in humans.http://link.springer.com/article/10.5625/lar.2016.32.2.99N-neuraminic acidglycomacropeptideHelicobacterantibacterial activity |
| spellingShingle | Dong-Jae Kim Min-Jung Kang Jin-A Choi Dae-Seung Na Jin-Beom Kim Chun-Soo Na Jong-Hwan Park Anti-Helicobacter pylori activity of crude N-acetylneuraminic acid isolated from glycomacropeptide of whey Laboratory Animal Research N-neuraminic acid glycomacropeptide Helicobacter antibacterial activity |
| title | Anti-Helicobacter pylori activity of crude N-acetylneuraminic acid isolated from glycomacropeptide of whey |
| title_full | Anti-Helicobacter pylori activity of crude N-acetylneuraminic acid isolated from glycomacropeptide of whey |
| title_fullStr | Anti-Helicobacter pylori activity of crude N-acetylneuraminic acid isolated from glycomacropeptide of whey |
| title_full_unstemmed | Anti-Helicobacter pylori activity of crude N-acetylneuraminic acid isolated from glycomacropeptide of whey |
| title_short | Anti-Helicobacter pylori activity of crude N-acetylneuraminic acid isolated from glycomacropeptide of whey |
| title_sort | anti helicobacter pylori activity of crude n acetylneuraminic acid isolated from glycomacropeptide of whey |
| topic | N-neuraminic acid glycomacropeptide Helicobacter antibacterial activity |
| url | http://link.springer.com/article/10.5625/lar.2016.32.2.99 |
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