An Alternative and Rapid Method for the Extraction of Nucleic Acids from Ixodid Ticks by Potassium Acetate Procedure

An Alternative and Rapid Method for the Extraction of Nucleic Acids from Ixodid Ticks by Potassium Acetate Procedure

Four variants of the potassium acetate procedure for DNA extraction from ixodid ticks at different stage of their life cycles were evaluated and compared with phenol-chloroform and ammonium hydroxide methods. The most rapid and most efficient variant was validated in the DNA extraction procedure fro...

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Bibliographic Details
Main Authors: Islay Rodríguez, Jorge Fraga, Angel Alberto Noda, Milagro Mayet, Yanisia Duarte, Eduardo Echevarria, Carmen Fernández
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2014-08-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Ticks
PCR
DNA extraction
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400542&lng=en&tlng=en

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