TRANSGENIC STRATEGY FOR IDENTIFYING SYNAPTIC CONNECTIONS IN MICE BY FLUORESCENCE COMPLEMENTATION (GRASP)
In the "GFP reconstitution across synaptic partners" (GRASP) method, non-fluorescent fragments of GFP are expressed in two different neurons; the fragments self-assemble at synapses between the two to form a fluorophore. GRASP has proven useful for light microscopic identification...
Main Authors: | Masahito eYamagata, Joshua R Sanes |
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Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2012-02-01
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Series: | Frontiers in Molecular Neuroscience |
Subjects: | |
Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnmol.2012.00018/full |
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