The role of tertiary lymphoid structure and B cells in nasopharyngeal carcinoma: Based on bioinformatics and experimental verification
Objective: Transcriptomic characteristics and prognosis of tertiary lymphoid structures (TLS) and infiltrating B cells in nasopharyngeal carcinoma (NPC) remain unclear. Here, NPC transcriptomic data and clinical samples were used to investigate the role of infiltrating B cells and TLS in NPC. Method...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-03-01
|
| Series: | Translational Oncology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1936523324000111 |
| _version_ | 1797324307740753920 |
|---|---|
| author | Chujun Chen Yan Zhang Xiaoting Wu Juan Shen |
| author_facet | Chujun Chen Yan Zhang Xiaoting Wu Juan Shen |
| author_sort | Chujun Chen |
| collection | DOAJ |
| description | Objective: Transcriptomic characteristics and prognosis of tertiary lymphoid structures (TLS) and infiltrating B cells in nasopharyngeal carcinoma (NPC) remain unclear. Here, NPC transcriptomic data and clinical samples were used to investigate the role of infiltrating B cells and TLS in NPC. Methods: We investigated the gene expression and infiltrating immune cells of NPC patients and further investigated the clinical relevance of B cell and TLS signatures. Transcriptional features of infiltrating B cell subsets were revealed by single-cell RNA sequencing (scRNA-seq) analysis. Immunohistochemical (IHC) and HE staining were performed to validate the clinical relevance of infiltrating B cells and TLS in NPC samples. Results: 27 differentially expressed immune-related genes (IRGs) associated with prognosis were identified, including B cell marker genes CD19 and CD79B. The higher B cells and TLS signature scores were associated with better outcomes and early pathological staging in 88 NPC patients. ScRNA-seq identified five distinct B cell subsets in NPC, including the BC-4 cluster associated with poor outcomes and the BC-0 cluster associated with better outcomes. EBV infection was positively associated with the formation of TLS. Furthermore, experimental results showed that the infiltration of B cells in NPC tissues was higher than that of normal tissues, and the density of TLS in an early stage of NPC was higher than that in advanced-stage TLS. Conclusion: Our findings demonstrate the functional importance of distinct B cell subsets in the prognosis of NPC. Additionally, we confirmed that B cells and TLS may serve as prognostic biomarkers of survival for NPC patients. |
| first_indexed | 2024-03-08T05:54:11Z |
| format | Article |
| id | doaj.art-00832aa2256b4b4098f2d64bf34031aa |
| institution | Directory Open Access Journal |
| issn | 1936-5233 |
| language | English |
| last_indexed | 2024-03-08T05:54:11Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Translational Oncology |
| spelling | doaj.art-00832aa2256b4b4098f2d64bf34031aa2024-02-05T04:31:39ZengElsevierTranslational Oncology1936-52332024-03-0141101885The role of tertiary lymphoid structure and B cells in nasopharyngeal carcinoma: Based on bioinformatics and experimental verificationChujun Chen0Yan Zhang1Xiaoting Wu2Juan Shen3Key Specialty of Clinical Pharmacy, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, PR ChinaPathology Dept., The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, 510006, PR ChinaSchool of Bioscience and Biopharmaceutics, Guangdong Province Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou, PR ChinaSchool of Bioscience and Biopharmaceutics, Guangdong Province Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou, PR China; Correspondence author.Objective: Transcriptomic characteristics and prognosis of tertiary lymphoid structures (TLS) and infiltrating B cells in nasopharyngeal carcinoma (NPC) remain unclear. Here, NPC transcriptomic data and clinical samples were used to investigate the role of infiltrating B cells and TLS in NPC. Methods: We investigated the gene expression and infiltrating immune cells of NPC patients and further investigated the clinical relevance of B cell and TLS signatures. Transcriptional features of infiltrating B cell subsets were revealed by single-cell RNA sequencing (scRNA-seq) analysis. Immunohistochemical (IHC) and HE staining were performed to validate the clinical relevance of infiltrating B cells and TLS in NPC samples. Results: 27 differentially expressed immune-related genes (IRGs) associated with prognosis were identified, including B cell marker genes CD19 and CD79B. The higher B cells and TLS signature scores were associated with better outcomes and early pathological staging in 88 NPC patients. ScRNA-seq identified five distinct B cell subsets in NPC, including the BC-4 cluster associated with poor outcomes and the BC-0 cluster associated with better outcomes. EBV infection was positively associated with the formation of TLS. Furthermore, experimental results showed that the infiltration of B cells in NPC tissues was higher than that of normal tissues, and the density of TLS in an early stage of NPC was higher than that in advanced-stage TLS. Conclusion: Our findings demonstrate the functional importance of distinct B cell subsets in the prognosis of NPC. Additionally, we confirmed that B cells and TLS may serve as prognostic biomarkers of survival for NPC patients.http://www.sciencedirect.com/science/article/pii/S1936523324000111Single cell sequencingNasopharyngeal carcinomaB cellsTertiary lymphoid structure |
| spellingShingle | Chujun Chen Yan Zhang Xiaoting Wu Juan Shen The role of tertiary lymphoid structure and B cells in nasopharyngeal carcinoma: Based on bioinformatics and experimental verification Translational Oncology Single cell sequencing Nasopharyngeal carcinoma B cells Tertiary lymphoid structure |
| title | The role of tertiary lymphoid structure and B cells in nasopharyngeal carcinoma: Based on bioinformatics and experimental verification |
| title_full | The role of tertiary lymphoid structure and B cells in nasopharyngeal carcinoma: Based on bioinformatics and experimental verification |
| title_fullStr | The role of tertiary lymphoid structure and B cells in nasopharyngeal carcinoma: Based on bioinformatics and experimental verification |
| title_full_unstemmed | The role of tertiary lymphoid structure and B cells in nasopharyngeal carcinoma: Based on bioinformatics and experimental verification |
| title_short | The role of tertiary lymphoid structure and B cells in nasopharyngeal carcinoma: Based on bioinformatics and experimental verification |
| title_sort | role of tertiary lymphoid structure and b cells in nasopharyngeal carcinoma based on bioinformatics and experimental verification |
| topic | Single cell sequencing Nasopharyngeal carcinoma B cells Tertiary lymphoid structure |
| url | http://www.sciencedirect.com/science/article/pii/S1936523324000111 |
| work_keys_str_mv | AT chujunchen theroleoftertiarylymphoidstructureandbcellsinnasopharyngealcarcinomabasedonbioinformaticsandexperimentalverification AT yanzhang theroleoftertiarylymphoidstructureandbcellsinnasopharyngealcarcinomabasedonbioinformaticsandexperimentalverification AT xiaotingwu theroleoftertiarylymphoidstructureandbcellsinnasopharyngealcarcinomabasedonbioinformaticsandexperimentalverification AT juanshen theroleoftertiarylymphoidstructureandbcellsinnasopharyngealcarcinomabasedonbioinformaticsandexperimentalverification AT chujunchen roleoftertiarylymphoidstructureandbcellsinnasopharyngealcarcinomabasedonbioinformaticsandexperimentalverification AT yanzhang roleoftertiarylymphoidstructureandbcellsinnasopharyngealcarcinomabasedonbioinformaticsandexperimentalverification AT xiaotingwu roleoftertiarylymphoidstructureandbcellsinnasopharyngealcarcinomabasedonbioinformaticsandexperimentalverification AT juanshen roleoftertiarylymphoidstructureandbcellsinnasopharyngealcarcinomabasedonbioinformaticsandexperimentalverification |