Antimicrobial Performance of an Innovative Technology of Atmospheric Plasma Reactors against Bioaerosols: Effectiveness in Removing Airborne Viable Viruses
Reducing the exposure to airborne contaminants, including bioaerosols containing viruses, is a key challenge in the context of indoor air quality. This study aims to assess the effectiveness of innovative Atmospheric Plasma Reactor (APR) technology, which can be included in air cleaner devices, as a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Buildings |
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| Online Access: | https://www.mdpi.com/2075-5309/12/10/1587 |
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| author | Jérémie Pourchez Aurélien Peyron Gwendoline Sarry Lara Leclerc Paul O. Verhoeven Peter Choi Claude Pierson Olivier Petit Francisco Hernández Carmen Dumitrescu |
| author_facet | Jérémie Pourchez Aurélien Peyron Gwendoline Sarry Lara Leclerc Paul O. Verhoeven Peter Choi Claude Pierson Olivier Petit Francisco Hernández Carmen Dumitrescu |
| author_sort | Jérémie Pourchez |
| collection | DOAJ |
| description | Reducing the exposure to airborne contaminants, including bioaerosols containing viruses, is a key challenge in the context of indoor air quality. This study aims to assess the effectiveness of innovative Atmospheric Plasma Reactor (APR) technology, which can be included in air cleaner devices, as an engineering control tool for reducing the concentration of viable airborne viruses. We investigated the KillViD<sup>TM</sup> APR technology that uses ultra-high electric fields and pulsed power plasma to directly electroporate living cells and produce advanced oxidizing species in situ within the micro-droplet aerosols containing the pathogens to be treated. An experimental setup was developed in order to aerosolize a high concentration of virus suspension directly into the air cleaner, containing 3 or 6 modules of 215 atmospheric plasma micro-reactors. As a virus surrogate, we used the phi11 bacteriophage which was aerosolized using a vibrating mesh nebulizer. The viability of airborne viruses after a single pass through the air cleaner was assessed by quantifying the lysis of a specific Staphylococcus aureus host strain. We were able to demonstrate that our virucidal results were robust and showed a 5-log reduction (99.999%) in terms of virucidal activity for the 3-module configuration, while we observed at least a 6-log reduction (from an initial viral load of 9.25 × 10<sup>5</sup> PFU to 0) for the 6-module configuration. |
| first_indexed | 2024-03-09T20:33:36Z |
| format | Article |
| id | doaj.art-00845d69eb7a4ce6b5365db199d16d84 |
| institution | Directory Open Access Journal |
| issn | 2075-5309 |
| language | English |
| last_indexed | 2024-03-09T20:33:36Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Buildings |
| spelling | doaj.art-00845d69eb7a4ce6b5365db199d16d842023-11-23T23:16:44ZengMDPI AGBuildings2075-53092022-10-011210158710.3390/buildings12101587Antimicrobial Performance of an Innovative Technology of Atmospheric Plasma Reactors against Bioaerosols: Effectiveness in Removing Airborne Viable VirusesJérémie Pourchez0Aurélien Peyron1Gwendoline Sarry2Lara Leclerc3Paul O. Verhoeven4Peter Choi5Claude Pierson6Olivier Petit7Francisco Hernández8Carmen Dumitrescu9Mines Saint-Etienne, University of Lyon, University Jean Monnet, INSERM, U 1059 Sainbiose, Centre CIS, 42023 Saint-Etienne, FranceMines Saint-Etienne, University of Lyon, University Jean Monnet, INSERM, U 1059 Sainbiose, Centre CIS, 42023 Saint-Etienne, FranceMines Saint-Etienne, University of Lyon, University Jean Monnet, INSERM, U 1059 Sainbiose, Centre CIS, 42023 Saint-Etienne, FranceMines Saint-Etienne, University of Lyon, University Jean Monnet, INSERM, U 1059 Sainbiose, Centre CIS, 42023 Saint-Etienne, FranceCIRI (Centre International de Recherche en Infectiologie), INSERM U111—CNRS UMR 5308—ENS de Lyon—UCB Lyon 1, Equipe GIMAP, Université Jean Monnet, 42100 Saint-Etienne, FranceGamma Pulse SAS, Bât 404, Ecole Polytechnique, 91128 Palaiseau, FranceGamma Pulse SAS, Bât 404, Ecole Polytechnique, 91128 Palaiseau, FranceGamma Pulse SAS, Bât 404, Ecole Polytechnique, 91128 Palaiseau, FranceGamma Pulse SAS, Bât 404, Ecole Polytechnique, 91128 Palaiseau, FranceGamma Pulse SAS, Bât 404, Ecole Polytechnique, 91128 Palaiseau, FranceReducing the exposure to airborne contaminants, including bioaerosols containing viruses, is a key challenge in the context of indoor air quality. This study aims to assess the effectiveness of innovative Atmospheric Plasma Reactor (APR) technology, which can be included in air cleaner devices, as an engineering control tool for reducing the concentration of viable airborne viruses. We investigated the KillViD<sup>TM</sup> APR technology that uses ultra-high electric fields and pulsed power plasma to directly electroporate living cells and produce advanced oxidizing species in situ within the micro-droplet aerosols containing the pathogens to be treated. An experimental setup was developed in order to aerosolize a high concentration of virus suspension directly into the air cleaner, containing 3 or 6 modules of 215 atmospheric plasma micro-reactors. As a virus surrogate, we used the phi11 bacteriophage which was aerosolized using a vibrating mesh nebulizer. The viability of airborne viruses after a single pass through the air cleaner was assessed by quantifying the lysis of a specific Staphylococcus aureus host strain. We were able to demonstrate that our virucidal results were robust and showed a 5-log reduction (99.999%) in terms of virucidal activity for the 3-module configuration, while we observed at least a 6-log reduction (from an initial viral load of 9.25 × 10<sup>5</sup> PFU to 0) for the 6-module configuration.https://www.mdpi.com/2075-5309/12/10/1587air cleanerpulsed poweratmospheric plasmabioaerosol inactivationbacteriophageindoor air quality |
| spellingShingle | Jérémie Pourchez Aurélien Peyron Gwendoline Sarry Lara Leclerc Paul O. Verhoeven Peter Choi Claude Pierson Olivier Petit Francisco Hernández Carmen Dumitrescu Antimicrobial Performance of an Innovative Technology of Atmospheric Plasma Reactors against Bioaerosols: Effectiveness in Removing Airborne Viable Viruses Buildings air cleaner pulsed power atmospheric plasma bioaerosol inactivation bacteriophage indoor air quality |
| title | Antimicrobial Performance of an Innovative Technology of Atmospheric Plasma Reactors against Bioaerosols: Effectiveness in Removing Airborne Viable Viruses |
| title_full | Antimicrobial Performance of an Innovative Technology of Atmospheric Plasma Reactors against Bioaerosols: Effectiveness in Removing Airborne Viable Viruses |
| title_fullStr | Antimicrobial Performance of an Innovative Technology of Atmospheric Plasma Reactors against Bioaerosols: Effectiveness in Removing Airborne Viable Viruses |
| title_full_unstemmed | Antimicrobial Performance of an Innovative Technology of Atmospheric Plasma Reactors against Bioaerosols: Effectiveness in Removing Airborne Viable Viruses |
| title_short | Antimicrobial Performance of an Innovative Technology of Atmospheric Plasma Reactors against Bioaerosols: Effectiveness in Removing Airborne Viable Viruses |
| title_sort | antimicrobial performance of an innovative technology of atmospheric plasma reactors against bioaerosols effectiveness in removing airborne viable viruses |
| topic | air cleaner pulsed power atmospheric plasma bioaerosol inactivation bacteriophage indoor air quality |
| url | https://www.mdpi.com/2075-5309/12/10/1587 |
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