<it>In vitro </it>fertilization and artificial activation of eggs of the direct-developing anuran <it>Eleutherodactylus coqui</it>
<p>Abstract</p> <p>Although much is known about the reproductive biology of pond-breeding frogs, there is comparatively little information about terrestrial-breeding anurans, a highly successful and diverse group. This study investigates the activation and <it>in vitro </i...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2004-08-01
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| Series: | Reproductive Biology and Endocrinology |
| Online Access: | http://www.rbej.com/content/2/1/60 |
| _version_ | 1811330121790914560 |
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| author | Toro Esteban Michael Scott F |
| author_facet | Toro Esteban Michael Scott F |
| author_sort | Toro Esteban |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Although much is known about the reproductive biology of pond-breeding frogs, there is comparatively little information about terrestrial-breeding anurans, a highly successful and diverse group. This study investigates the activation and <it>in vitro </it>fertilization of eggs of the Puerto Rican coqui frog obtained by hormonally induced ovulation. We report that spontaneous activation occurs in 34% of eggs, probably in response to mechanical stress during oviposition. Artificial activation, as evidenced by the slow block to polyspermy and the onset of zygote division, was elicited both by mechanical stimulation and calcium ionophore exposure in 64% and 83% of the cases, respectively. Finally, one <it>in vitro </it>fertilization protocol showed a 27% success rate, despite the fact that about one third of all unfertilized eggs obtained by hormone injection auto-activate. We expect these findings to aid in the conservation effort of <it>Eleutherodactylus </it>frogs, the largest vertebrate genus.</p> |
| first_indexed | 2024-04-13T15:55:51Z |
| format | Article |
| id | doaj.art-0085779948fd45f0a3451277ac9d378e |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-04-13T15:55:51Z |
| publishDate | 2004-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-0085779948fd45f0a3451277ac9d378e2022-12-22T02:40:41ZengBMCReproductive Biology and Endocrinology1477-78272004-08-01216010.1186/1477-7827-2-60<it>In vitro </it>fertilization and artificial activation of eggs of the direct-developing anuran <it>Eleutherodactylus coqui</it>Toro EstebanMichael Scott F<p>Abstract</p> <p>Although much is known about the reproductive biology of pond-breeding frogs, there is comparatively little information about terrestrial-breeding anurans, a highly successful and diverse group. This study investigates the activation and <it>in vitro </it>fertilization of eggs of the Puerto Rican coqui frog obtained by hormonally induced ovulation. We report that spontaneous activation occurs in 34% of eggs, probably in response to mechanical stress during oviposition. Artificial activation, as evidenced by the slow block to polyspermy and the onset of zygote division, was elicited both by mechanical stimulation and calcium ionophore exposure in 64% and 83% of the cases, respectively. Finally, one <it>in vitro </it>fertilization protocol showed a 27% success rate, despite the fact that about one third of all unfertilized eggs obtained by hormone injection auto-activate. We expect these findings to aid in the conservation effort of <it>Eleutherodactylus </it>frogs, the largest vertebrate genus.</p>http://www.rbej.com/content/2/1/60 |
| spellingShingle | Toro Esteban Michael Scott F <it>In vitro </it>fertilization and artificial activation of eggs of the direct-developing anuran <it>Eleutherodactylus coqui</it> Reproductive Biology and Endocrinology |
| title | <it>In vitro </it>fertilization and artificial activation of eggs of the direct-developing anuran <it>Eleutherodactylus coqui</it> |
| title_full | <it>In vitro </it>fertilization and artificial activation of eggs of the direct-developing anuran <it>Eleutherodactylus coqui</it> |
| title_fullStr | <it>In vitro </it>fertilization and artificial activation of eggs of the direct-developing anuran <it>Eleutherodactylus coqui</it> |
| title_full_unstemmed | <it>In vitro </it>fertilization and artificial activation of eggs of the direct-developing anuran <it>Eleutherodactylus coqui</it> |
| title_short | <it>In vitro </it>fertilization and artificial activation of eggs of the direct-developing anuran <it>Eleutherodactylus coqui</it> |
| title_sort | it in vitro it fertilization and artificial activation of eggs of the direct developing anuran it eleutherodactylus coqui it |
| url | http://www.rbej.com/content/2/1/60 |
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