Bovine Colostrum Supplementation Improves Bone Metabolism in an Osteoporosis-Induced Animal Model
Osteoporosis is characterized by bone loss. The present study aims to investigate the effects of bovine colostrum (BC) on bone metabolism using ovariectomized (OVX) and orchidectomized (ORX) rat models. Twenty-seven-week-old Wistar Han rats were randomly assigned as: (1) placebo control, (2) BC supp...
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MDPI AG
2021-08-01
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| author | Eirini K. Kydonaki Laura Freitas Bruno M. Fonseca Henrique Reguengo Carlos Raposo Simón Ana R. Bastos Emanuel M. Fernandes Raphaël F. Canadas Joaquim Miguel Oliveira Vitor M. Correlo Rui L. Reis Maria Vliora Parakevi Gkiata Yiannis Koutedakis Georgia Ntina Rui Pinto Andres E. Carrillo Franklim Marques Tânia Amorim |
| author_facet | Eirini K. Kydonaki Laura Freitas Bruno M. Fonseca Henrique Reguengo Carlos Raposo Simón Ana R. Bastos Emanuel M. Fernandes Raphaël F. Canadas Joaquim Miguel Oliveira Vitor M. Correlo Rui L. Reis Maria Vliora Parakevi Gkiata Yiannis Koutedakis Georgia Ntina Rui Pinto Andres E. Carrillo Franklim Marques Tânia Amorim |
| author_sort | Eirini K. Kydonaki |
| collection | DOAJ |
| description | Osteoporosis is characterized by bone loss. The present study aims to investigate the effects of bovine colostrum (BC) on bone metabolism using ovariectomized (OVX) and orchidectomized (ORX) rat models. Twenty-seven-week-old Wistar Han rats were randomly assigned as: (1) placebo control, (2) BC supplementation dose 1 (BC1: 0.5 g/day/OVX, 1 g/day/ORX), (3) BC supplementation dose 2 (BC2: 1 g/day/OVX, 1.5 g/day/ORX) and (4) BC supplementation dose 3 (BC3: 1.5 g/day/OVX, 2 g/day/ORX). Bone microarchitecture, strength, gene expression of VEGFA, FGF2, RANKL, RANK and OPG, and bone resorption/formation markers were assessed after four months of BC supplementation. Compared to the placebo, OVX rats in the BC1 group exhibited significantly higher cortical bone mineral content and trabecular bone mineral content (<i>p</i> < 0.01), while OVX rats in the BC3 group showed significantly higher trabecular bone mineral content (<i>p</i> < 0.05). ORX rats receiving BC dose 2 demonstrated significantly higher levels of trabecular bone mineral content (<i>p</i> < 0.05). Serum osteocalcin in the ORX was pointedly higher in all BC supplementation groups than the placebo (BC1: <i>p</i> < 0.05; BC2, BC3: <i>p</i> < 0.001). Higher doses of BC induced significantly higher relative mRNA expression of OPG, VEGFA, FGF2 and RANKL (<i>p</i> < 0.05). BC supplementation improves bone metabolism of OVX and ORX rats, which might be associated with the activation of the VEGFA, FGF2 and RANKL/RANK/OPG pathways. |
| first_indexed | 2024-03-10T07:20:50Z |
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| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T07:20:50Z |
| publishDate | 2021-08-01 |
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| series | Nutrients |
| spelling | doaj.art-008c1b01eaf844d6ad22cbba0ea8f9322023-11-22T14:35:27ZengMDPI AGNutrients2072-66432021-08-01139298110.3390/nu13092981Bovine Colostrum Supplementation Improves Bone Metabolism in an Osteoporosis-Induced Animal ModelEirini K. Kydonaki0Laura Freitas1Bruno M. Fonseca2Henrique Reguengo3Carlos Raposo Simón4Ana R. Bastos5Emanuel M. Fernandes6Raphaël F. Canadas7Joaquim Miguel Oliveira8Vitor M. Correlo9Rui L. Reis10Maria Vliora11Parakevi Gkiata12Yiannis Koutedakis13Georgia Ntina14Rui Pinto15Andres E. Carrillo16Franklim Marques17Tânia Amorim18UCIBIO/REQUIMTE, Faculty of Pharmacy, University of Porto, 4050-313 Porto, PortugalUCIBIO/REQUIMTE, Faculty of Pharmacy, University of Porto, 4050-313 Porto, PortugalUCIBIO/REQUIMTE, Faculty of Pharmacy, University of Porto, 4050-313 Porto, PortugalUCIBIO/REQUIMTE, Faculty of Pharmacy, University of Porto, 4050-313 Porto, PortugalSALURIS, 28108 Madrid, Spain3B’s Research Group, I3Bs—Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Guimarães, Portugal3B’s Research Group, I3Bs—Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Guimarães, Portugal3B’s Research Group, I3Bs—Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Guimarães, Portugal3B’s Research Group, I3Bs—Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Guimarães, Portugal3B’s Research Group, I3Bs—Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Guimarães, Portugal3B’s Research Group, I3Bs—Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Guimarães, PortugalSchool of Sports and Exercise Sciences, University of Thessaly, 42100 Trikala, GreeceSchool of Sports and Exercise Sciences, University of Thessaly, 42100 Trikala, GreeceSchool of Sports and Exercise Sciences, University of Thessaly, 42100 Trikala, GreeceBME, Biomechanical Solutions, 43150 Karditsa, GreeceiMed.UL, Faculty of Pharmacy, University of Lisbon, 1649-003 Lisbon, PortugalDepartment of Exercise Science, Chatham University, Pittsburgh, PA 15232, USAUCIBIO/REQUIMTE, Faculty of Pharmacy, University of Porto, 4050-313 Porto, PortugalUCIBIO/REQUIMTE, Faculty of Pharmacy, University of Porto, 4050-313 Porto, PortugalOsteoporosis is characterized by bone loss. The present study aims to investigate the effects of bovine colostrum (BC) on bone metabolism using ovariectomized (OVX) and orchidectomized (ORX) rat models. Twenty-seven-week-old Wistar Han rats were randomly assigned as: (1) placebo control, (2) BC supplementation dose 1 (BC1: 0.5 g/day/OVX, 1 g/day/ORX), (3) BC supplementation dose 2 (BC2: 1 g/day/OVX, 1.5 g/day/ORX) and (4) BC supplementation dose 3 (BC3: 1.5 g/day/OVX, 2 g/day/ORX). Bone microarchitecture, strength, gene expression of VEGFA, FGF2, RANKL, RANK and OPG, and bone resorption/formation markers were assessed after four months of BC supplementation. Compared to the placebo, OVX rats in the BC1 group exhibited significantly higher cortical bone mineral content and trabecular bone mineral content (<i>p</i> < 0.01), while OVX rats in the BC3 group showed significantly higher trabecular bone mineral content (<i>p</i> < 0.05). ORX rats receiving BC dose 2 demonstrated significantly higher levels of trabecular bone mineral content (<i>p</i> < 0.05). Serum osteocalcin in the ORX was pointedly higher in all BC supplementation groups than the placebo (BC1: <i>p</i> < 0.05; BC2, BC3: <i>p</i> < 0.001). Higher doses of BC induced significantly higher relative mRNA expression of OPG, VEGFA, FGF2 and RANKL (<i>p</i> < 0.05). BC supplementation improves bone metabolism of OVX and ORX rats, which might be associated with the activation of the VEGFA, FGF2 and RANKL/RANK/OPG pathways.https://www.mdpi.com/2072-6643/13/9/2981bovine colostrumboneosteoporosissupplementation |
| spellingShingle | Eirini K. Kydonaki Laura Freitas Bruno M. Fonseca Henrique Reguengo Carlos Raposo Simón Ana R. Bastos Emanuel M. Fernandes Raphaël F. Canadas Joaquim Miguel Oliveira Vitor M. Correlo Rui L. Reis Maria Vliora Parakevi Gkiata Yiannis Koutedakis Georgia Ntina Rui Pinto Andres E. Carrillo Franklim Marques Tânia Amorim Bovine Colostrum Supplementation Improves Bone Metabolism in an Osteoporosis-Induced Animal Model Nutrients bovine colostrum bone osteoporosis supplementation |
| title | Bovine Colostrum Supplementation Improves Bone Metabolism in an Osteoporosis-Induced Animal Model |
| title_full | Bovine Colostrum Supplementation Improves Bone Metabolism in an Osteoporosis-Induced Animal Model |
| title_fullStr | Bovine Colostrum Supplementation Improves Bone Metabolism in an Osteoporosis-Induced Animal Model |
| title_full_unstemmed | Bovine Colostrum Supplementation Improves Bone Metabolism in an Osteoporosis-Induced Animal Model |
| title_short | Bovine Colostrum Supplementation Improves Bone Metabolism in an Osteoporosis-Induced Animal Model |
| title_sort | bovine colostrum supplementation improves bone metabolism in an osteoporosis induced animal model |
| topic | bovine colostrum bone osteoporosis supplementation |
| url | https://www.mdpi.com/2072-6643/13/9/2981 |
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