GhFAD2–3 is required for anther development in Gossypium hirsutum
Abstract Background In higher plants, the FAD2 gene encodes the microsomal oleate Δ12-desaturase, one of the key enzymes essential for the biosynthesis of the polyunsaturated lipids that serve many important functions in plant development and stress responses. FAD2 catalyzes the first step, in the b...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-09-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12870-019-2010-9 |
| _version_ | 1819044827962015744 |
|---|---|
| author | Feng Liu Lihong Ma Youwu Wang Yanjun Li Xinyu Zhang Fei Xue Xinhui Nie Qianhao Zhu Jie Sun |
| author_facet | Feng Liu Lihong Ma Youwu Wang Yanjun Li Xinyu Zhang Fei Xue Xinhui Nie Qianhao Zhu Jie Sun |
| author_sort | Feng Liu |
| collection | DOAJ |
| description | Abstract Background In higher plants, the FAD2 gene encodes the microsomal oleate Δ12-desaturase, one of the key enzymes essential for the biosynthesis of the polyunsaturated lipids that serve many important functions in plant development and stress responses. FAD2 catalyzes the first step, in the biosynthesis of the polyunsaturated fatty acids (PUFAs) found in the cell membrane and cell wall, and it is thus of great importance to investigate the regulatory role of FAD2 in anther development. Results We reported the molecular characterization of the cotton (Gossypium hirsutum) GhFAD2 gene family and the essential role of GhFAD2–3 in cotton anther development. G. hirsutum contains four pairs of homoeologous FAD2 genes (GhFAD2–1 to GhFAD2–4). GhFAD2–3 is ubiquitously and relatively highly expressed in all analyzed tissues, particularly in anthers. Specific inhibition of GhFAD2–3 using the RNA interference approach resulted in male sterility due to impaired anther development at the stages from meiosis to maturation. The cellular phenotypic abnormality observed at the meiosis stage of the GhFAD2–3 silenced plant (fad2–3) coincides with the significant reduction of C18:2 in anthers at the same stage. Compared with that of the wild type (WT), the content of C18:1 was 41.48%, which increased by 5 fold in the fad2–3 anther at the pollen maturation stage. Moreover, the ratio of monounsaturated to polyunsaturated fatty acid was 5.43 in fad2–3 anther, which was much higher than that of the WT (only 0.39). Through compositional analysis of anthers cuticle and transcriptome data, we demonstrated it was unfavorable to the development of anther by regulating GhFAD2–3 expression level to increase the oleic acid content. Conclusions Our work demonstrated the importance of C18:2 and/or C18:3 in the development of the pollen exine and anther cuticle in cotton and provided clue for further investigation of the physiological significance of the fatty acid composition for plant growth and development. |
| first_indexed | 2024-12-21T10:18:52Z |
| format | Article |
| id | doaj.art-008d29b8b75f4685b3487c9b4f841824 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-21T10:18:52Z |
| publishDate | 2019-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-008d29b8b75f4685b3487c9b4f8418242022-12-21T19:07:31ZengBMCBMC Plant Biology1471-22292019-09-0119111710.1186/s12870-019-2010-9GhFAD2–3 is required for anther development in Gossypium hirsutumFeng Liu0Lihong Ma1Youwu Wang2Yanjun Li3Xinyu Zhang4Fei Xue5Xinhui Nie6Qianhao Zhu7Jie Sun8Key Laboratory of Oasis Eco-agriculture, College of Agriculture, Shihezi UniversityKey Laboratory of Oasis Eco-agriculture, College of Agriculture, Shihezi UniversityCollege of Plant Science and Technology, Tarim UniversityKey Laboratory of Oasis Eco-agriculture, College of Agriculture, Shihezi UniversityKey Laboratory of Oasis Eco-agriculture, College of Agriculture, Shihezi UniversityKey Laboratory of Oasis Eco-agriculture, College of Agriculture, Shihezi UniversityKey Laboratory of Oasis Eco-agriculture, College of Agriculture, Shihezi UniversityCSIRO Agriculture and FoodKey Laboratory of Oasis Eco-agriculture, College of Agriculture, Shihezi UniversityAbstract Background In higher plants, the FAD2 gene encodes the microsomal oleate Δ12-desaturase, one of the key enzymes essential for the biosynthesis of the polyunsaturated lipids that serve many important functions in plant development and stress responses. FAD2 catalyzes the first step, in the biosynthesis of the polyunsaturated fatty acids (PUFAs) found in the cell membrane and cell wall, and it is thus of great importance to investigate the regulatory role of FAD2 in anther development. Results We reported the molecular characterization of the cotton (Gossypium hirsutum) GhFAD2 gene family and the essential role of GhFAD2–3 in cotton anther development. G. hirsutum contains four pairs of homoeologous FAD2 genes (GhFAD2–1 to GhFAD2–4). GhFAD2–3 is ubiquitously and relatively highly expressed in all analyzed tissues, particularly in anthers. Specific inhibition of GhFAD2–3 using the RNA interference approach resulted in male sterility due to impaired anther development at the stages from meiosis to maturation. The cellular phenotypic abnormality observed at the meiosis stage of the GhFAD2–3 silenced plant (fad2–3) coincides with the significant reduction of C18:2 in anthers at the same stage. Compared with that of the wild type (WT), the content of C18:1 was 41.48%, which increased by 5 fold in the fad2–3 anther at the pollen maturation stage. Moreover, the ratio of monounsaturated to polyunsaturated fatty acid was 5.43 in fad2–3 anther, which was much higher than that of the WT (only 0.39). Through compositional analysis of anthers cuticle and transcriptome data, we demonstrated it was unfavorable to the development of anther by regulating GhFAD2–3 expression level to increase the oleic acid content. Conclusions Our work demonstrated the importance of C18:2 and/or C18:3 in the development of the pollen exine and anther cuticle in cotton and provided clue for further investigation of the physiological significance of the fatty acid composition for plant growth and development.http://link.springer.com/article/10.1186/s12870-019-2010-9Gossypium hirsutumFatty acid compositionGhFAD2Male sterileLinoleic acidOleic acid |
| spellingShingle | Feng Liu Lihong Ma Youwu Wang Yanjun Li Xinyu Zhang Fei Xue Xinhui Nie Qianhao Zhu Jie Sun GhFAD2–3 is required for anther development in Gossypium hirsutum BMC Plant Biology Gossypium hirsutum Fatty acid composition GhFAD2 Male sterile Linoleic acid Oleic acid |
| title | GhFAD2–3 is required for anther development in Gossypium hirsutum |
| title_full | GhFAD2–3 is required for anther development in Gossypium hirsutum |
| title_fullStr | GhFAD2–3 is required for anther development in Gossypium hirsutum |
| title_full_unstemmed | GhFAD2–3 is required for anther development in Gossypium hirsutum |
| title_short | GhFAD2–3 is required for anther development in Gossypium hirsutum |
| title_sort | ghfad2 3 is required for anther development in gossypium hirsutum |
| topic | Gossypium hirsutum Fatty acid composition GhFAD2 Male sterile Linoleic acid Oleic acid |
| url | http://link.springer.com/article/10.1186/s12870-019-2010-9 |
| work_keys_str_mv | AT fengliu ghfad23isrequiredforantherdevelopmentingossypiumhirsutum AT lihongma ghfad23isrequiredforantherdevelopmentingossypiumhirsutum AT youwuwang ghfad23isrequiredforantherdevelopmentingossypiumhirsutum AT yanjunli ghfad23isrequiredforantherdevelopmentingossypiumhirsutum AT xinyuzhang ghfad23isrequiredforantherdevelopmentingossypiumhirsutum AT feixue ghfad23isrequiredforantherdevelopmentingossypiumhirsutum AT xinhuinie ghfad23isrequiredforantherdevelopmentingossypiumhirsutum AT qianhaozhu ghfad23isrequiredforantherdevelopmentingossypiumhirsutum AT jiesun ghfad23isrequiredforantherdevelopmentingossypiumhirsutum |