Dissecting the Root Phenotypic and Genotypic Variability of the Iowa Mung Bean Diversity Panel
Mung bean [Vigna radiata (L.) Wilczek] is a drought-tolerant, short-duration crop, and a rich source of protein and other valuable minerals, vitamins, and antioxidants. The main objectives of this research were (1) to study the root traits related with the phenotypic and genetic diversity of 375 mun...
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| Format: | Article |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.808001/full |
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| author | Kevin O. Chiteri Talukder Zaki Jubery Somak Dutta Baskar Ganapathysubramanian Steven Cannon Steven Cannon Arti Singh |
| author_facet | Kevin O. Chiteri Talukder Zaki Jubery Somak Dutta Baskar Ganapathysubramanian Steven Cannon Steven Cannon Arti Singh |
| author_sort | Kevin O. Chiteri |
| collection | DOAJ |
| description | Mung bean [Vigna radiata (L.) Wilczek] is a drought-tolerant, short-duration crop, and a rich source of protein and other valuable minerals, vitamins, and antioxidants. The main objectives of this research were (1) to study the root traits related with the phenotypic and genetic diversity of 375 mung bean genotypes of the Iowa (IA) diversity panel and (2) to conduct genome-wide association studies of root-related traits using the Automated Root Image Analysis (ARIA) software. We collected over 9,000 digital images at three-time points (days 12, 15, and 18 after germination). A broad sense heritability for days 15 (0.22–0.73) and 18 (0.23–0.87) was higher than that for day 12 (0.24–0.51). We also reported root ideotype classification, i.e., PI425425 (India), PI425045 (Philippines), PI425551 (Korea), PI264686 (Philippines), and PI425085 (Sri Lanka) that emerged as the top five in the topsoil foraging category, while PI425594 (unknown origin), PI425599 (Thailand), PI425610 (Afghanistan), PI425485 (India), and AVMU0201 (Taiwan) were top five in the drought-tolerant and nutrient uptake “steep, cheap, and deep” ideotype. We identified promising genotypes that can help diversify the gene pool of mung bean breeding stocks and will be useful for further field testing. Using association studies, we identified markers showing significant associations with the lateral root angle (LRA) on chromosomes 2, 6, 7, and 11, length distribution (LED) on chromosome 8, and total root length-growth rate (TRL_GR), volume (VOL), and total dry weight (TDW) on chromosomes 3 and 5. We discussed genes that are potential candidates from these regions. We reported beta-galactosidase 3 associated with the LRA, which has previously been implicated in the adventitious root development via transcriptomic studies in mung bean. Results from this work on the phenotypic characterization, root-based ideotype categories, and significant molecular markers associated with important traits will be useful for the marker-assisted selection and mung bean improvement through breeding. |
| first_indexed | 2024-12-17T17:36:49Z |
| format | Article |
| id | doaj.art-00906ad2299f4756ac6cb15c52d5dec7 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
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| last_indexed | 2024-12-17T17:36:49Z |
| publishDate | 2022-01-01 |
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| spelling | doaj.art-00906ad2299f4756ac6cb15c52d5dec72022-12-21T21:39:18ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-01-011210.3389/fpls.2021.808001808001Dissecting the Root Phenotypic and Genotypic Variability of the Iowa Mung Bean Diversity PanelKevin O. Chiteri0Talukder Zaki Jubery1Somak Dutta2Baskar Ganapathysubramanian3Steven Cannon4Steven Cannon5Arti Singh6Department of Agronomy, Iowa State University, Ames, IA, United StatesDepartment of Mechanical Engineering, Iowa State University, Ames, IA, United StatesDepartment of Statistics, Iowa State University, Ames, IA, United StatesDepartment of Mechanical Engineering, Iowa State University, Ames, IA, United StatesDepartment of Agronomy, Iowa State University, Ames, IA, United StatesUSDA—Agricultural Research Service, Corn Insects and Crop Genetics Research Unit, Ames, IA, United StatesDepartment of Agronomy, Iowa State University, Ames, IA, United StatesMung bean [Vigna radiata (L.) Wilczek] is a drought-tolerant, short-duration crop, and a rich source of protein and other valuable minerals, vitamins, and antioxidants. The main objectives of this research were (1) to study the root traits related with the phenotypic and genetic diversity of 375 mung bean genotypes of the Iowa (IA) diversity panel and (2) to conduct genome-wide association studies of root-related traits using the Automated Root Image Analysis (ARIA) software. We collected over 9,000 digital images at three-time points (days 12, 15, and 18 after germination). A broad sense heritability for days 15 (0.22–0.73) and 18 (0.23–0.87) was higher than that for day 12 (0.24–0.51). We also reported root ideotype classification, i.e., PI425425 (India), PI425045 (Philippines), PI425551 (Korea), PI264686 (Philippines), and PI425085 (Sri Lanka) that emerged as the top five in the topsoil foraging category, while PI425594 (unknown origin), PI425599 (Thailand), PI425610 (Afghanistan), PI425485 (India), and AVMU0201 (Taiwan) were top five in the drought-tolerant and nutrient uptake “steep, cheap, and deep” ideotype. We identified promising genotypes that can help diversify the gene pool of mung bean breeding stocks and will be useful for further field testing. Using association studies, we identified markers showing significant associations with the lateral root angle (LRA) on chromosomes 2, 6, 7, and 11, length distribution (LED) on chromosome 8, and total root length-growth rate (TRL_GR), volume (VOL), and total dry weight (TDW) on chromosomes 3 and 5. We discussed genes that are potential candidates from these regions. We reported beta-galactosidase 3 associated with the LRA, which has previously been implicated in the adventitious root development via transcriptomic studies in mung bean. Results from this work on the phenotypic characterization, root-based ideotype categories, and significant molecular markers associated with important traits will be useful for the marker-assisted selection and mung bean improvement through breeding.https://www.frontiersin.org/articles/10.3389/fpls.2021.808001/fullroot system architectureGWAShigh throughput phenotypingphenomicspulses |
| spellingShingle | Kevin O. Chiteri Talukder Zaki Jubery Somak Dutta Baskar Ganapathysubramanian Steven Cannon Steven Cannon Arti Singh Dissecting the Root Phenotypic and Genotypic Variability of the Iowa Mung Bean Diversity Panel Frontiers in Plant Science root system architecture GWAS high throughput phenotyping phenomics pulses |
| title | Dissecting the Root Phenotypic and Genotypic Variability of the Iowa Mung Bean Diversity Panel |
| title_full | Dissecting the Root Phenotypic and Genotypic Variability of the Iowa Mung Bean Diversity Panel |
| title_fullStr | Dissecting the Root Phenotypic and Genotypic Variability of the Iowa Mung Bean Diversity Panel |
| title_full_unstemmed | Dissecting the Root Phenotypic and Genotypic Variability of the Iowa Mung Bean Diversity Panel |
| title_short | Dissecting the Root Phenotypic and Genotypic Variability of the Iowa Mung Bean Diversity Panel |
| title_sort | dissecting the root phenotypic and genotypic variability of the iowa mung bean diversity panel |
| topic | root system architecture GWAS high throughput phenotyping phenomics pulses |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.808001/full |
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