Determinants of vaccine immunogenicity in HIV-infected pregnant women: analysis of B and T cell responses to pandemic H1N1 monovalent vaccine.
Influenza infections have high frequency and morbidity in HIV-infected pregnant women, underscoring the importance of vaccine-conferred protection. To identify the factors that determine vaccine immunogenicity in this group, we characterized the relationship of B- and T-cell responses to pandemic H1...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4395240?pdf=render |
| _version_ | 1811191592836399104 |
|---|---|
| author | Adriana Weinberg Petronella Muresan Kelly M Richardson Terence Fenton Teresa Dominguez Anthony Bloom D Heather Watts Mark J Abzug Sharon A Nachman Myron J Levin P1086 team |
| author_facet | Adriana Weinberg Petronella Muresan Kelly M Richardson Terence Fenton Teresa Dominguez Anthony Bloom D Heather Watts Mark J Abzug Sharon A Nachman Myron J Levin P1086 team |
| author_sort | Adriana Weinberg |
| collection | DOAJ |
| description | Influenza infections have high frequency and morbidity in HIV-infected pregnant women, underscoring the importance of vaccine-conferred protection. To identify the factors that determine vaccine immunogenicity in this group, we characterized the relationship of B- and T-cell responses to pandemic H1N1 (pH1N1) vaccine with HIV-associated immunologic and virologic characteristics. pH1N1 and seasonal-H1N1 (sH1N1) antibodies were measured in 119 HIV-infected pregnant women after two double-strength pH1N1 vaccine doses. pH1N1-IgG and IgA B-cell FluoroSpot, pH1N1- and sH1N1-interferon γ (IFNγ) and granzyme B (GrB) T-cell FluoroSpot, and flow cytometric characterization of B- and T-cell subsets were performed in 57 subjects. pH1N1-antibodies increased after vaccination, but less than previously described in healthy adults. pH1N1-IgG memory B cells (Bmem) increased, IFNγ-effector T-cells (Teff) decreased, and IgA Bmem and GrB Teff did not change. pH1N1-antibodies and Teff were significantly correlated with each other and with sH1N1-HAI and Teff, respectively, before and after vaccination. pH1N1-antibody responses to the vaccine significantly increased with high proportions of CD4+, low CD8+ and low CD8+HLADR+CD38+ activated (Tact) cells. pH1N1-IgG Bmem responses increased with high proportions of CD19+CD27+CD21- activated B cells (Bact), high CD8+CD39+ regulatory T cells (Treg), and low CD19+CD27-CD21- exhausted B cells (Bexhaust). IFNγ-Teff responses increased with low HIV plasma RNA, CD8+HLADR+CD38+ Tact, CD4+FoxP3+ Treg and CD19+IL10+ Breg. In conclusion, pre-existing antibody and Teff responses to sH1N1 were associated with increased responses to pH1N1 vaccination in HIV-infected pregnant women suggesting an important role for heterosubtypic immunologic memory. High CD4+% T cells were associated with increased, whereas high HIV replication, Tact and Bexhaust were associated with decreased vaccine immunogenicity. High Treg increased antibody responses but decreased Teff responses to the vaccine. The proportions of immature and transitional B cells did not affect the responses to vaccine. Increased Bact were associated with high Bmem responses to the vaccine. |
| first_indexed | 2024-04-11T23:39:39Z |
| format | Article |
| id | doaj.art-00935d1b30344d51b5813e623e45568f |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T23:39:39Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-00935d1b30344d51b5813e623e45568f2022-12-22T03:56:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01104e012243110.1371/journal.pone.0122431Determinants of vaccine immunogenicity in HIV-infected pregnant women: analysis of B and T cell responses to pandemic H1N1 monovalent vaccine.Adriana WeinbergPetronella MuresanKelly M RichardsonTerence FentonTeresa DominguezAnthony BloomD Heather WattsMark J AbzugSharon A NachmanMyron J LevinP1086 teamInfluenza infections have high frequency and morbidity in HIV-infected pregnant women, underscoring the importance of vaccine-conferred protection. To identify the factors that determine vaccine immunogenicity in this group, we characterized the relationship of B- and T-cell responses to pandemic H1N1 (pH1N1) vaccine with HIV-associated immunologic and virologic characteristics. pH1N1 and seasonal-H1N1 (sH1N1) antibodies were measured in 119 HIV-infected pregnant women after two double-strength pH1N1 vaccine doses. pH1N1-IgG and IgA B-cell FluoroSpot, pH1N1- and sH1N1-interferon γ (IFNγ) and granzyme B (GrB) T-cell FluoroSpot, and flow cytometric characterization of B- and T-cell subsets were performed in 57 subjects. pH1N1-antibodies increased after vaccination, but less than previously described in healthy adults. pH1N1-IgG memory B cells (Bmem) increased, IFNγ-effector T-cells (Teff) decreased, and IgA Bmem and GrB Teff did not change. pH1N1-antibodies and Teff were significantly correlated with each other and with sH1N1-HAI and Teff, respectively, before and after vaccination. pH1N1-antibody responses to the vaccine significantly increased with high proportions of CD4+, low CD8+ and low CD8+HLADR+CD38+ activated (Tact) cells. pH1N1-IgG Bmem responses increased with high proportions of CD19+CD27+CD21- activated B cells (Bact), high CD8+CD39+ regulatory T cells (Treg), and low CD19+CD27-CD21- exhausted B cells (Bexhaust). IFNγ-Teff responses increased with low HIV plasma RNA, CD8+HLADR+CD38+ Tact, CD4+FoxP3+ Treg and CD19+IL10+ Breg. In conclusion, pre-existing antibody and Teff responses to sH1N1 were associated with increased responses to pH1N1 vaccination in HIV-infected pregnant women suggesting an important role for heterosubtypic immunologic memory. High CD4+% T cells were associated with increased, whereas high HIV replication, Tact and Bexhaust were associated with decreased vaccine immunogenicity. High Treg increased antibody responses but decreased Teff responses to the vaccine. The proportions of immature and transitional B cells did not affect the responses to vaccine. Increased Bact were associated with high Bmem responses to the vaccine.http://europepmc.org/articles/PMC4395240?pdf=render |
| spellingShingle | Adriana Weinberg Petronella Muresan Kelly M Richardson Terence Fenton Teresa Dominguez Anthony Bloom D Heather Watts Mark J Abzug Sharon A Nachman Myron J Levin P1086 team Determinants of vaccine immunogenicity in HIV-infected pregnant women: analysis of B and T cell responses to pandemic H1N1 monovalent vaccine. PLoS ONE |
| title | Determinants of vaccine immunogenicity in HIV-infected pregnant women: analysis of B and T cell responses to pandemic H1N1 monovalent vaccine. |
| title_full | Determinants of vaccine immunogenicity in HIV-infected pregnant women: analysis of B and T cell responses to pandemic H1N1 monovalent vaccine. |
| title_fullStr | Determinants of vaccine immunogenicity in HIV-infected pregnant women: analysis of B and T cell responses to pandemic H1N1 monovalent vaccine. |
| title_full_unstemmed | Determinants of vaccine immunogenicity in HIV-infected pregnant women: analysis of B and T cell responses to pandemic H1N1 monovalent vaccine. |
| title_short | Determinants of vaccine immunogenicity in HIV-infected pregnant women: analysis of B and T cell responses to pandemic H1N1 monovalent vaccine. |
| title_sort | determinants of vaccine immunogenicity in hiv infected pregnant women analysis of b and t cell responses to pandemic h1n1 monovalent vaccine |
| url | http://europepmc.org/articles/PMC4395240?pdf=render |
| work_keys_str_mv | AT adrianaweinberg determinantsofvaccineimmunogenicityinhivinfectedpregnantwomenanalysisofbandtcellresponsestopandemich1n1monovalentvaccine AT petronellamuresan determinantsofvaccineimmunogenicityinhivinfectedpregnantwomenanalysisofbandtcellresponsestopandemich1n1monovalentvaccine AT kellymrichardson determinantsofvaccineimmunogenicityinhivinfectedpregnantwomenanalysisofbandtcellresponsestopandemich1n1monovalentvaccine AT terencefenton determinantsofvaccineimmunogenicityinhivinfectedpregnantwomenanalysisofbandtcellresponsestopandemich1n1monovalentvaccine AT teresadominguez determinantsofvaccineimmunogenicityinhivinfectedpregnantwomenanalysisofbandtcellresponsestopandemich1n1monovalentvaccine AT anthonybloom determinantsofvaccineimmunogenicityinhivinfectedpregnantwomenanalysisofbandtcellresponsestopandemich1n1monovalentvaccine AT dheatherwatts determinantsofvaccineimmunogenicityinhivinfectedpregnantwomenanalysisofbandtcellresponsestopandemich1n1monovalentvaccine AT markjabzug determinantsofvaccineimmunogenicityinhivinfectedpregnantwomenanalysisofbandtcellresponsestopandemich1n1monovalentvaccine AT sharonanachman determinantsofvaccineimmunogenicityinhivinfectedpregnantwomenanalysisofbandtcellresponsestopandemich1n1monovalentvaccine AT myronjlevin determinantsofvaccineimmunogenicityinhivinfectedpregnantwomenanalysisofbandtcellresponsestopandemich1n1monovalentvaccine AT p1086team determinantsofvaccineimmunogenicityinhivinfectedpregnantwomenanalysisofbandtcellresponsestopandemich1n1monovalentvaccine |