Diagnostic challenges of single plaque-like lesion paucibacillary leprosy
The diagnosis of single-lesion paucibacillary leprosy remains a challenge. Reviews by expert dermatopathologists and quantitative polymerase chain reaction (qPCR) results obtained from 66 single-plaque biopsy samples were compared. Histological findings were graded as high (HP), medium (MP) or low (...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
2014-11-01
|
| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000700944&lng=en&tlng=en |
| _version_ | 1827847676258942976 |
|---|---|
| author | Raquel Rodrigues Barbieri Anna Maria Sales Ximena Illarramendi Milton Ozório Moraes José Augusto da Costa Nery Suelen Justo Maria Moreira Euzenir Nunes Sarno Alice de Miranda Machado Fernando Augusto Bozza |
| author_facet | Raquel Rodrigues Barbieri Anna Maria Sales Ximena Illarramendi Milton Ozório Moraes José Augusto da Costa Nery Suelen Justo Maria Moreira Euzenir Nunes Sarno Alice de Miranda Machado Fernando Augusto Bozza |
| author_sort | Raquel Rodrigues Barbieri |
| collection | DOAJ |
| description | The diagnosis of single-lesion paucibacillary leprosy remains a challenge. Reviews by expert dermatopathologists and quantitative polymerase chain reaction (qPCR) results obtained from 66 single-plaque biopsy samples were compared. Histological findings were graded as high (HP), medium (MP) or low (LP) probability of leprosy or other dermatopathy (OD). Mycobacterium leprae-specific genes were detected using qPCR. The biopsies of 47 out of 57 clinically diagnosed patients who received multidrug therapy were classified as HP/MP, eight of which were qPCR negative. In the LP/OD (n = 19), two out of eight untreated patients showed positive qPCR results. In the absence of typical histopathological features, qPCR may be utilised to aid in final patient diagnosis, thus reducing overtreatment and delay in diagnosis. |
| first_indexed | 2024-03-12T09:33:42Z |
| format | Article |
| id | doaj.art-00943b75af2a4590ad0bdce082faa6a6 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T09:33:42Z |
| publishDate | 2014-11-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-00943b75af2a4590ad0bdce082faa6a62023-09-02T13:48:58ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602014-11-01109794494710.1590/0074-0276140212S0074-02762014000700944Diagnostic challenges of single plaque-like lesion paucibacillary leprosyRaquel Rodrigues BarbieriAnna Maria SalesXimena IllarramendiMilton Ozório MoraesJosé Augusto da Costa NerySuelen Justo Maria MoreiraEuzenir Nunes SarnoAlice de Miranda MachadoFernando Augusto BozzaThe diagnosis of single-lesion paucibacillary leprosy remains a challenge. Reviews by expert dermatopathologists and quantitative polymerase chain reaction (qPCR) results obtained from 66 single-plaque biopsy samples were compared. Histological findings were graded as high (HP), medium (MP) or low (LP) probability of leprosy or other dermatopathy (OD). Mycobacterium leprae-specific genes were detected using qPCR. The biopsies of 47 out of 57 clinically diagnosed patients who received multidrug therapy were classified as HP/MP, eight of which were qPCR negative. In the LP/OD (n = 19), two out of eight untreated patients showed positive qPCR results. In the absence of typical histopathological features, qPCR may be utilised to aid in final patient diagnosis, thus reducing overtreatment and delay in diagnosis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000700944&lng=en&tlng=enpaucibacillary leprosydiagnosisquantitative polymerase chain reaction |
| spellingShingle | Raquel Rodrigues Barbieri Anna Maria Sales Ximena Illarramendi Milton Ozório Moraes José Augusto da Costa Nery Suelen Justo Maria Moreira Euzenir Nunes Sarno Alice de Miranda Machado Fernando Augusto Bozza Diagnostic challenges of single plaque-like lesion paucibacillary leprosy Memorias do Instituto Oswaldo Cruz paucibacillary leprosy diagnosis quantitative polymerase chain reaction |
| title | Diagnostic challenges of single plaque-like lesion paucibacillary leprosy |
| title_full | Diagnostic challenges of single plaque-like lesion paucibacillary leprosy |
| title_fullStr | Diagnostic challenges of single plaque-like lesion paucibacillary leprosy |
| title_full_unstemmed | Diagnostic challenges of single plaque-like lesion paucibacillary leprosy |
| title_short | Diagnostic challenges of single plaque-like lesion paucibacillary leprosy |
| title_sort | diagnostic challenges of single plaque like lesion paucibacillary leprosy |
| topic | paucibacillary leprosy diagnosis quantitative polymerase chain reaction |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000700944&lng=en&tlng=en |
| work_keys_str_mv | AT raquelrodriguesbarbieri diagnosticchallengesofsingleplaquelikelesionpaucibacillaryleprosy AT annamariasales diagnosticchallengesofsingleplaquelikelesionpaucibacillaryleprosy AT ximenaillarramendi diagnosticchallengesofsingleplaquelikelesionpaucibacillaryleprosy AT miltonozoriomoraes diagnosticchallengesofsingleplaquelikelesionpaucibacillaryleprosy AT joseaugustodacostanery diagnosticchallengesofsingleplaquelikelesionpaucibacillaryleprosy AT suelenjustomariamoreira diagnosticchallengesofsingleplaquelikelesionpaucibacillaryleprosy AT euzenirnunessarno diagnosticchallengesofsingleplaquelikelesionpaucibacillaryleprosy AT alicedemirandamachado diagnosticchallengesofsingleplaquelikelesionpaucibacillaryleprosy AT fernandoaugustobozza diagnosticchallengesofsingleplaquelikelesionpaucibacillaryleprosy |