Developing a New Biophysical Tool to Combine Magneto-Optical Tweezers with Super-Resolution Fluorescence Microscopy
We present a novel experimental setup in which magnetic and optical tweezers are combined for torque and force transduction onto single filamentous molecules in a transverse configuration to allow simultaneous mechanical measurement and manipulation. Previously we have developed a super-resolution i...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2015-06-01
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| Series: | Photonics |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2304-6732/2/3/758 |
| _version_ | 1818159321777700864 |
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| author | Zhaokun Zhou Helen Miller Adam J.M. Wollman Mark C. Leake |
| author_facet | Zhaokun Zhou Helen Miller Adam J.M. Wollman Mark C. Leake |
| author_sort | Zhaokun Zhou |
| collection | DOAJ |
| description | We present a novel experimental setup in which magnetic and optical tweezers are combined for torque and force transduction onto single filamentous molecules in a transverse configuration to allow simultaneous mechanical measurement and manipulation. Previously we have developed a super-resolution imaging module which, in conjunction with advanced imaging techniques such as Blinking assisted Localisation Microscopy (BaLM), achieves localisation precision of single fluorescent dye molecules bound to DNA of ~30 nm along the contour of the molecule; our work here describes developments in producing a system which combines tweezing and super-resolution fluorescence imaging. The instrument also features an acousto-optic deflector that temporally divides the laser beam to form multiple traps for high throughput statistics collection. Our motivation for developing the new tool is to enable direct observation of detailed molecular topological transformation and protein binding event localisation in a stretching/twisting mechanical assay that previously could hitherto only be deduced indirectly from the end-to-end length variation of DNA. Our approach is simple and robust enough for reproduction in the lab without the requirement of precise hardware engineering, yet is capable of unveiling the elastic and dynamic properties of filamentous molecules that have been hidden using traditional tools. |
| first_indexed | 2024-12-11T15:44:08Z |
| format | Article |
| id | doaj.art-0098e03b69824d919ba3cea9425e8bc9 |
| institution | Directory Open Access Journal |
| issn | 2304-6732 |
| language | English |
| last_indexed | 2024-12-11T15:44:08Z |
| publishDate | 2015-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Photonics |
| spelling | doaj.art-0098e03b69824d919ba3cea9425e8bc92022-12-22T00:59:44ZengMDPI AGPhotonics2304-67322015-06-012375877210.3390/photonics2030758photonics2030758Developing a New Biophysical Tool to Combine Magneto-Optical Tweezers with Super-Resolution Fluorescence MicroscopyZhaokun Zhou0Helen Miller1Adam J.M. Wollman2Mark C. Leake3Departments of Physics and Biology, Biological Physical Sciences Institute (BPSI), University of York, York YO10 5DD, UKDepartments of Physics and Biology, Biological Physical Sciences Institute (BPSI), University of York, York YO10 5DD, UKDepartments of Physics and Biology, Biological Physical Sciences Institute (BPSI), University of York, York YO10 5DD, UKDepartments of Physics and Biology, Biological Physical Sciences Institute (BPSI), University of York, York YO10 5DD, UKWe present a novel experimental setup in which magnetic and optical tweezers are combined for torque and force transduction onto single filamentous molecules in a transverse configuration to allow simultaneous mechanical measurement and manipulation. Previously we have developed a super-resolution imaging module which, in conjunction with advanced imaging techniques such as Blinking assisted Localisation Microscopy (BaLM), achieves localisation precision of single fluorescent dye molecules bound to DNA of ~30 nm along the contour of the molecule; our work here describes developments in producing a system which combines tweezing and super-resolution fluorescence imaging. The instrument also features an acousto-optic deflector that temporally divides the laser beam to form multiple traps for high throughput statistics collection. Our motivation for developing the new tool is to enable direct observation of detailed molecular topological transformation and protein binding event localisation in a stretching/twisting mechanical assay that previously could hitherto only be deduced indirectly from the end-to-end length variation of DNA. Our approach is simple and robust enough for reproduction in the lab without the requirement of precise hardware engineering, yet is capable of unveiling the elastic and dynamic properties of filamentous molecules that have been hidden using traditional tools.http://www.mdpi.com/2304-6732/2/3/758optical tweezersmagnetic tweezerssuper-resolution microscopy |
| spellingShingle | Zhaokun Zhou Helen Miller Adam J.M. Wollman Mark C. Leake Developing a New Biophysical Tool to Combine Magneto-Optical Tweezers with Super-Resolution Fluorescence Microscopy Photonics optical tweezers magnetic tweezers super-resolution microscopy |
| title | Developing a New Biophysical Tool to Combine Magneto-Optical Tweezers with Super-Resolution Fluorescence Microscopy |
| title_full | Developing a New Biophysical Tool to Combine Magneto-Optical Tweezers with Super-Resolution Fluorescence Microscopy |
| title_fullStr | Developing a New Biophysical Tool to Combine Magneto-Optical Tweezers with Super-Resolution Fluorescence Microscopy |
| title_full_unstemmed | Developing a New Biophysical Tool to Combine Magneto-Optical Tweezers with Super-Resolution Fluorescence Microscopy |
| title_short | Developing a New Biophysical Tool to Combine Magneto-Optical Tweezers with Super-Resolution Fluorescence Microscopy |
| title_sort | developing a new biophysical tool to combine magneto optical tweezers with super resolution fluorescence microscopy |
| topic | optical tweezers magnetic tweezers super-resolution microscopy |
| url | http://www.mdpi.com/2304-6732/2/3/758 |
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