Erianin alleviates collagen-induced arthritis in mice by inhibiting Th17 cell differentiation
Rheumatoid arthritis (RA) is a chronic autoimmune disorder. Its pathogenesis is complicated but highly related to aberrant Th17 overactivation. Uncontrolled Th17 cell expansion and activation in populations and associated activities contribute to the progression of RA. Although clinical RA remedies...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2023-09-01
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| Series: | Open Life Sciences |
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| Online Access: | https://doi.org/10.1515/biol-2022-0703 |
| _version_ | 1827820965861523456 |
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| author | Tsai Sen-Wei Wang Jou-Hsuan Chang Yu-Kang Lin Chi-Chen |
| author_facet | Tsai Sen-Wei Wang Jou-Hsuan Chang Yu-Kang Lin Chi-Chen |
| author_sort | Tsai Sen-Wei |
| collection | DOAJ |
| description | Rheumatoid arthritis (RA) is a chronic autoimmune disorder. Its pathogenesis is complicated but highly related to aberrant Th17 overactivation. Uncontrolled Th17 cell expansion and activation in populations and associated activities contribute to the progression of RA. Although clinical RA remedies are available, not all RA patients respond to these treatments, and adverse effects are always a concerning issue during treatment. To expand the repertoire of possible anti-RA remedies, we chose the phytochemical compound erianin, isolated from Dendrobium sp., and evaluated its antiarthritic effect in vitro and in vivo. We found that erianin efficiently controlled the differentiation and activation of Th17 cell development from primary CD4 T cells, limiting IL-17A cytokine production and RORγT transcript generation. In line with molecular docking models, the essential signaling pathway for Th17 polarization, the JAK/STAT3 pathway, was inhibited upon erianin treatment, with dose-dependent inhibition of phosphorylation shown by western blotting. More importantly, erianin treatment reduced arthritic manifestations and proinflammatory cytokine levels in collagen-induced arthritis (CIA) mice, as well as protecting the joint histological microstructure. Overall, erianin revealed a promising inhibitory effect on Th17 overactivation and decreased disability in CIA mice. Therefore, erianin could be further developed as a candidate RA remedy. |
| first_indexed | 2024-03-12T01:35:45Z |
| format | Article |
| id | doaj.art-0098f619613840bbb635288189a01f49 |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-03-12T01:35:45Z |
| publishDate | 2023-09-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj.art-0098f619613840bbb635288189a01f492023-09-11T06:59:14ZengDe GruyterOpen Life Sciences2391-54122023-09-0118112334010.1515/biol-2022-0703Erianin alleviates collagen-induced arthritis in mice by inhibiting Th17 cell differentiationTsai Sen-Wei0Wang Jou-Hsuan1Chang Yu-Kang2Lin Chi-Chen3Department of Physical Medicine and Rehabilitation, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taichung 427, TaiwanDepartment of Physical Medicine and Rehabilitation, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taichung 427, TaiwanDepartment of Medical Research, Tungs’ Taichung MetroHarbor Hospital, Taichung 435, TaiwanInstitute of Biomedical Science, National Chung Hsing University, Taichung, Taichung 402, TaiwanRheumatoid arthritis (RA) is a chronic autoimmune disorder. Its pathogenesis is complicated but highly related to aberrant Th17 overactivation. Uncontrolled Th17 cell expansion and activation in populations and associated activities contribute to the progression of RA. Although clinical RA remedies are available, not all RA patients respond to these treatments, and adverse effects are always a concerning issue during treatment. To expand the repertoire of possible anti-RA remedies, we chose the phytochemical compound erianin, isolated from Dendrobium sp., and evaluated its antiarthritic effect in vitro and in vivo. We found that erianin efficiently controlled the differentiation and activation of Th17 cell development from primary CD4 T cells, limiting IL-17A cytokine production and RORγT transcript generation. In line with molecular docking models, the essential signaling pathway for Th17 polarization, the JAK/STAT3 pathway, was inhibited upon erianin treatment, with dose-dependent inhibition of phosphorylation shown by western blotting. More importantly, erianin treatment reduced arthritic manifestations and proinflammatory cytokine levels in collagen-induced arthritis (CIA) mice, as well as protecting the joint histological microstructure. Overall, erianin revealed a promising inhibitory effect on Th17 overactivation and decreased disability in CIA mice. Therefore, erianin could be further developed as a candidate RA remedy.https://doi.org/10.1515/biol-2022-0703rheumatoid arthritiscollagen-induced arthritisth17 cellerianinnatural compoundinflammation |
| spellingShingle | Tsai Sen-Wei Wang Jou-Hsuan Chang Yu-Kang Lin Chi-Chen Erianin alleviates collagen-induced arthritis in mice by inhibiting Th17 cell differentiation Open Life Sciences rheumatoid arthritis collagen-induced arthritis th17 cell erianin natural compound inflammation |
| title | Erianin alleviates collagen-induced arthritis in mice by inhibiting Th17 cell differentiation |
| title_full | Erianin alleviates collagen-induced arthritis in mice by inhibiting Th17 cell differentiation |
| title_fullStr | Erianin alleviates collagen-induced arthritis in mice by inhibiting Th17 cell differentiation |
| title_full_unstemmed | Erianin alleviates collagen-induced arthritis in mice by inhibiting Th17 cell differentiation |
| title_short | Erianin alleviates collagen-induced arthritis in mice by inhibiting Th17 cell differentiation |
| title_sort | erianin alleviates collagen induced arthritis in mice by inhibiting th17 cell differentiation |
| topic | rheumatoid arthritis collagen-induced arthritis th17 cell erianin natural compound inflammation |
| url | https://doi.org/10.1515/biol-2022-0703 |
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