Organization of acetylcholine containing structures in the cranial motor nuclei of the rhombencephalon of the pig
We explored the immunoreactivity of choline acetyltransferase (ChAT) in the cranial nerve motor nuclei of the porcine rhombencephalon to reveal the cholinergic nature of these regions. In our experiments we used an immunohistochemical method for the visualization of all acetylcholine-containing stru...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2007-07-01
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| Series: | Veterinární Medicína |
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| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-200707-0002_organization-of-acetylcholine-containing-structures-in-the-cranial-motor-nuclei-of-the-rhombencephalon-of-the-p.php |
| _version_ | 1797897702500990976 |
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| author | M. Zalecki J. Calka M. Lakomy |
| author_facet | M. Zalecki J. Calka M. Lakomy |
| author_sort | M. Zalecki |
| collection | DOAJ |
| description | We explored the immunoreactivity of choline acetyltransferase (ChAT) in the cranial nerve motor nuclei of the porcine rhombencephalon to reveal the cholinergic nature of these regions. In our experiments we used an immunohistochemical method for the visualization of all acetylcholine-containing structures. All studied motor nuclei contained ChAT-positive cell bodies and fibres, but the intensity of staining differed between the nuclei. Furthermore, characteristic ChAT-immunoreactive bouton-like structures, which are known to be synaptic terminals of the cholinergic system, were observed in the borders of all studied regions. The localization of ChAT-positive "boutons" in the neuropil of the examined nuclei and their proximity to stained perikarya allowed us to differentiate two groups of motor nuclei in the rhombencephalon of the pig: (a) Nuclei containing ChAT-positive bouton-like structures dispersed in the neuropil, often establishing contacts with the stained cell bodies - motor trigeminal, abducent, facial, ambiguous and hypoglossal nuclei. (b) Nuclei in which characteristic boutons were dispersed among the ChAT-positive cells, but were devoid of any contact with perikarya - dorsal motor nucleus of the vagus nerve. These results provide new data on the porcine central nervous system and could be useful in further experiments on amyotrophic lateral sclerosis (ALS) - the disease that results in the progressive degeneration of motoneurons in the brain and spinal cord. |
| first_indexed | 2024-04-10T08:01:26Z |
| format | Article |
| id | doaj.art-0099b182721e469a9201379769a46cdc |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T08:01:26Z |
| publishDate | 2007-07-01 |
| publisher | Czech Academy of Agricultural Sciences |
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| series | Veterinární Medicína |
| spelling | doaj.art-0099b182721e469a9201379769a46cdc2023-02-23T03:49:27ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922007-07-0152729330010.17221/2041-VETMEDvet-200707-0002Organization of acetylcholine containing structures in the cranial motor nuclei of the rhombencephalon of the pigM. Zalecki0J. Calka1M. Lakomy2University of Warmia and Mazury, Olsztyn, PolandUniversity of Warmia and Mazury, Olsztyn, PolandUniversity of Warmia and Mazury, Olsztyn, PolandWe explored the immunoreactivity of choline acetyltransferase (ChAT) in the cranial nerve motor nuclei of the porcine rhombencephalon to reveal the cholinergic nature of these regions. In our experiments we used an immunohistochemical method for the visualization of all acetylcholine-containing structures. All studied motor nuclei contained ChAT-positive cell bodies and fibres, but the intensity of staining differed between the nuclei. Furthermore, characteristic ChAT-immunoreactive bouton-like structures, which are known to be synaptic terminals of the cholinergic system, were observed in the borders of all studied regions. The localization of ChAT-positive "boutons" in the neuropil of the examined nuclei and their proximity to stained perikarya allowed us to differentiate two groups of motor nuclei in the rhombencephalon of the pig: (a) Nuclei containing ChAT-positive bouton-like structures dispersed in the neuropil, often establishing contacts with the stained cell bodies - motor trigeminal, abducent, facial, ambiguous and hypoglossal nuclei. (b) Nuclei in which characteristic boutons were dispersed among the ChAT-positive cells, but were devoid of any contact with perikarya - dorsal motor nucleus of the vagus nerve. These results provide new data on the porcine central nervous system and could be useful in further experiments on amyotrophic lateral sclerosis (ALS) - the disease that results in the progressive degeneration of motoneurons in the brain and spinal cord.https://vetmed.agriculturejournals.cz/artkey/vet-200707-0002_organization-of-acetylcholine-containing-structures-in-the-cranial-motor-nuclei-of-the-rhombencephalon-of-the-p.phpcranial nerve motor nucleicholine acetyltransferaseimmunoreactivityswine |
| spellingShingle | M. Zalecki J. Calka M. Lakomy Organization of acetylcholine containing structures in the cranial motor nuclei of the rhombencephalon of the pig Veterinární Medicína cranial nerve motor nuclei choline acetyltransferase immunoreactivity swine |
| title | Organization of acetylcholine containing structures in the cranial motor nuclei of the rhombencephalon of the pig |
| title_full | Organization of acetylcholine containing structures in the cranial motor nuclei of the rhombencephalon of the pig |
| title_fullStr | Organization of acetylcholine containing structures in the cranial motor nuclei of the rhombencephalon of the pig |
| title_full_unstemmed | Organization of acetylcholine containing structures in the cranial motor nuclei of the rhombencephalon of the pig |
| title_short | Organization of acetylcholine containing structures in the cranial motor nuclei of the rhombencephalon of the pig |
| title_sort | organization of acetylcholine containing structures in the cranial motor nuclei of the rhombencephalon of the pig |
| topic | cranial nerve motor nuclei choline acetyltransferase immunoreactivity swine |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-200707-0002_organization-of-acetylcholine-containing-structures-in-the-cranial-motor-nuclei-of-the-rhombencephalon-of-the-p.php |
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