Specific radiation damage is a lesser concern at room temperature
Carrying out macromolecular crystallography (MX) experiments at cryogenic temperatures significantly slows the rate of global radiation damage, thus facilitating the solution of high-resolution crystal structures of macromolecules. However, cryo-MX experiments suffer from the early onset of so-calle...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
International Union of Crystallography
2019-07-01
|
| Series: | IUCrJ |
| Subjects: | |
| Online Access: | http://scripts.iucr.org/cgi-bin/paper?S205225251900616X |
| _version_ | 1819281692228059136 |
|---|---|
| author | Guillaume Gotthard Sylvain Aumonier Daniele De Sanctis Gordon Leonard David von Stetten Antoine Royant |
| author_facet | Guillaume Gotthard Sylvain Aumonier Daniele De Sanctis Gordon Leonard David von Stetten Antoine Royant |
| author_sort | Guillaume Gotthard |
| collection | DOAJ |
| description | Carrying out macromolecular crystallography (MX) experiments at cryogenic temperatures significantly slows the rate of global radiation damage, thus facilitating the solution of high-resolution crystal structures of macromolecules. However, cryo-MX experiments suffer from the early onset of so-called specific radiation damage that affects certain amino-acid residues and, in particular, the active sites of many proteins. Here, a series of MX experiments are described which suggest that specific and global radiation damage are much less decoupled at room temperature than they are at cryogenic temperatures. The results reported here demonstrate the interest in reviving the practice of collecting MX diffraction data at room temperature and allow structural biologists to favourably envisage the development of time-resolved MX experiments at synchrotron sources. |
| first_indexed | 2024-12-24T01:03:44Z |
| format | Article |
| id | doaj.art-0099c3ca4b3041a48756dd84e750d7dc |
| institution | Directory Open Access Journal |
| issn | 2052-2525 |
| language | English |
| last_indexed | 2024-12-24T01:03:44Z |
| publishDate | 2019-07-01 |
| publisher | International Union of Crystallography |
| record_format | Article |
| series | IUCrJ |
| spelling | doaj.art-0099c3ca4b3041a48756dd84e750d7dc2022-12-21T17:23:17ZengInternational Union of CrystallographyIUCrJ2052-25252019-07-016466568010.1107/S205225251900616Xmf5033Specific radiation damage is a lesser concern at room temperatureGuillaume Gotthard0Sylvain Aumonier1Daniele De Sanctis2Gordon Leonard3David von Stetten4Antoine Royant5European Synchrotron Radiation Facility, F-38043 Grenoble, FranceEuropean Synchrotron Radiation Facility, F-38043 Grenoble, FranceEuropean Synchrotron Radiation Facility, F-38043 Grenoble, FranceEuropean Synchrotron Radiation Facility, F-38043 Grenoble, FranceEuropean Synchrotron Radiation Facility, F-38043 Grenoble, FranceEuropean Synchrotron Radiation Facility, F-38043 Grenoble, FranceCarrying out macromolecular crystallography (MX) experiments at cryogenic temperatures significantly slows the rate of global radiation damage, thus facilitating the solution of high-resolution crystal structures of macromolecules. However, cryo-MX experiments suffer from the early onset of so-called specific radiation damage that affects certain amino-acid residues and, in particular, the active sites of many proteins. Here, a series of MX experiments are described which suggest that specific and global radiation damage are much less decoupled at room temperature than they are at cryogenic temperatures. The results reported here demonstrate the interest in reviving the practice of collecting MX diffraction data at room temperature and allow structural biologists to favourably envisage the development of time-resolved MX experiments at synchrotron sources.http://scripts.iucr.org/cgi-bin/paper?S205225251900616Xroom-temperature macromolecular crystallographycryocrystallographyspecific radiation damagetime-resolved crystallography |
| spellingShingle | Guillaume Gotthard Sylvain Aumonier Daniele De Sanctis Gordon Leonard David von Stetten Antoine Royant Specific radiation damage is a lesser concern at room temperature IUCrJ room-temperature macromolecular crystallography cryocrystallography specific radiation damage time-resolved crystallography |
| title | Specific radiation damage is a lesser concern at room temperature |
| title_full | Specific radiation damage is a lesser concern at room temperature |
| title_fullStr | Specific radiation damage is a lesser concern at room temperature |
| title_full_unstemmed | Specific radiation damage is a lesser concern at room temperature |
| title_short | Specific radiation damage is a lesser concern at room temperature |
| title_sort | specific radiation damage is a lesser concern at room temperature |
| topic | room-temperature macromolecular crystallography cryocrystallography specific radiation damage time-resolved crystallography |
| url | http://scripts.iucr.org/cgi-bin/paper?S205225251900616X |
| work_keys_str_mv | AT guillaumegotthard specificradiationdamageisalesserconcernatroomtemperature AT sylvainaumonier specificradiationdamageisalesserconcernatroomtemperature AT danieledesanctis specificradiationdamageisalesserconcernatroomtemperature AT gordonleonard specificradiationdamageisalesserconcernatroomtemperature AT davidvonstetten specificradiationdamageisalesserconcernatroomtemperature AT antoineroyant specificradiationdamageisalesserconcernatroomtemperature |