Microsatellite Primer Development for Post Oak, <i>Quercus stellata</i> (Fagaceae)
Premise of the study: The American Cross Timbers forest ecosystem runs from southeastern Kansas to Central Texas and is primarily composed of post oak (<i>Quercus stellata</i>). This old-growth forest currently occupies only about 2% of its ancestral range. To facilitate genetic research...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-10-01
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| Series: | Applications in Plant Sciences |
| Subjects: | |
| Online Access: | http://www.bioone.org/doi/full/10.3732/apps.1400070 |
| _version_ | 1818245350581862400 |
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| author | Warren B. Chatwin Kyrie K. Carpenter Felix R. Jimenez Dave B. Elzinga Leigh A. Johnson Peter J. Maughan |
| author_facet | Warren B. Chatwin Kyrie K. Carpenter Felix R. Jimenez Dave B. Elzinga Leigh A. Johnson Peter J. Maughan |
| author_sort | Warren B. Chatwin |
| collection | DOAJ |
| description | Premise of the study: The American Cross Timbers forest ecosystem runs from southeastern Kansas to Central Texas and is primarily composed of post oak (<i>Quercus stellata</i>). This old-growth forest currently occupies only about 2% of its ancestral range. To facilitate genetic research on this species, we developed microsatellite primers specific to post oak from reduced genomic libraries.
Methods and Results: Two <i>Q. stellata</i> individuals, sampled from the northern and southern range of the post oak forest, were subject to genomic reduction and 454 pyrosequencing. Bioinformatic analysis identified putative microsatellites from which 12 polymorphic primer sets were screened on three populations. The number of alleles observed ranged from five to 20 across all populations, while observed and expected heterozygosity values ranged from 0.05 to 0.833 and 0.236 to 0.893, respectively, within individual populations.
Conclusions: We report the development of microsatellite markers, specific to post oak, to aid the study of genetic diversity and population structure of extant forest remnants. |
| first_indexed | 2024-12-12T14:31:31Z |
| format | Article |
| id | doaj.art-009a3cd53e574525926f9a50f97888cf |
| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-12-12T14:31:31Z |
| publishDate | 2014-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-009a3cd53e574525926f9a50f97888cf2022-12-22T00:21:30ZengWileyApplications in Plant Sciences2168-04502014-10-01210140007010.3732/apps.1400070Microsatellite Primer Development for Post Oak, <i>Quercus stellata</i> (Fagaceae)Warren B. Chatwin0Kyrie K. Carpenter1Felix R. Jimenez2Dave B. Elzinga3Leigh A. Johnson4Peter J. Maughan5Department of Plant and Wildlife Sciences, Brigham Young University, 5144 Life Sciences Building, Provo, Utah 84602 USADepartment of Plant and Wildlife Sciences, Brigham Young University, 5144 Life Sciences Building, Provo, Utah 84602 USADepartment of Plant and Wildlife Sciences, Brigham Young University, 5144 Life Sciences Building, Provo, Utah 84602 USADepartment of Plant and Wildlife Sciences, Brigham Young University, 5144 Life Sciences Building, Provo, Utah 84602 USADepartment of Biology, Brigham Young University, 4058 Life Sciences Building, Provo, Utah 84602 USADepartment of Plant and Wildlife Sciences, Brigham Young University, 5144 Life Sciences Building, Provo, Utah 84602 USAPremise of the study: The American Cross Timbers forest ecosystem runs from southeastern Kansas to Central Texas and is primarily composed of post oak (<i>Quercus stellata</i>). This old-growth forest currently occupies only about 2% of its ancestral range. To facilitate genetic research on this species, we developed microsatellite primers specific to post oak from reduced genomic libraries. Methods and Results: Two <i>Q. stellata</i> individuals, sampled from the northern and southern range of the post oak forest, were subject to genomic reduction and 454 pyrosequencing. Bioinformatic analysis identified putative microsatellites from which 12 polymorphic primer sets were screened on three populations. The number of alleles observed ranged from five to 20 across all populations, while observed and expected heterozygosity values ranged from 0.05 to 0.833 and 0.236 to 0.893, respectively, within individual populations. Conclusions: We report the development of microsatellite markers, specific to post oak, to aid the study of genetic diversity and population structure of extant forest remnants.http://www.bioone.org/doi/full/10.3732/apps.1400070454 pyrosequencingCross Timbersold-growth forestpost oakQuercus stellatasimple sequence repeat |
| spellingShingle | Warren B. Chatwin Kyrie K. Carpenter Felix R. Jimenez Dave B. Elzinga Leigh A. Johnson Peter J. Maughan Microsatellite Primer Development for Post Oak, <i>Quercus stellata</i> (Fagaceae) Applications in Plant Sciences 454 pyrosequencing Cross Timbers old-growth forest post oak Quercus stellata simple sequence repeat |
| title | Microsatellite Primer Development for Post Oak, <i>Quercus stellata</i> (Fagaceae) |
| title_full | Microsatellite Primer Development for Post Oak, <i>Quercus stellata</i> (Fagaceae) |
| title_fullStr | Microsatellite Primer Development for Post Oak, <i>Quercus stellata</i> (Fagaceae) |
| title_full_unstemmed | Microsatellite Primer Development for Post Oak, <i>Quercus stellata</i> (Fagaceae) |
| title_short | Microsatellite Primer Development for Post Oak, <i>Quercus stellata</i> (Fagaceae) |
| title_sort | microsatellite primer development for post oak i quercus stellata i fagaceae |
| topic | 454 pyrosequencing Cross Timbers old-growth forest post oak Quercus stellata simple sequence repeat |
| url | http://www.bioone.org/doi/full/10.3732/apps.1400070 |
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