Comparison of KIT patterns and infiltration of eosinophils in canine mast cell tumor
ABSTRACT KIT protein is associated with the etiology of canine mast cell tumors (MCT); however, the expression patterns of KIT are highly variable. The aim of this study was to determine if KIT patterns are related with eosinophil count in MCT. Hematoxylin eosin and May Grünwald-Giemsa stain techniq...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal de Minas Gerais
2023-11-01
|
| Series: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352023000601047&lng=en&tlng=en |
| _version_ | 1797628687830482944 |
|---|---|
| author | C.D. Araújo G.S. Sanches F. Borek D.C. Rocha G.D. Giustina J.R. Engracia Filho G.H. Bechara |
| author_facet | C.D. Araújo G.S. Sanches F. Borek D.C. Rocha G.D. Giustina J.R. Engracia Filho G.H. Bechara |
| author_sort | C.D. Araújo |
| collection | DOAJ |
| description | ABSTRACT KIT protein is associated with the etiology of canine mast cell tumors (MCT); however, the expression patterns of KIT are highly variable. The aim of this study was to determine if KIT patterns are related with eosinophil count in MCT. Hematoxylin eosin and May Grünwald-Giemsa stain techniques were applied, histological grading and eosinophil counting were performed in 48 MCT samples. Immunohistochemical evaluation was performed with IL-5, VEGFr, and c-KIT antibodies. The percentage of immunolabeling with IL-5 and VEGFr was determined, and the samples incubated with c-KIT were graded according to the immunolabeling pattern. Comparison of the mean eosinophil count between the histological grades and the different KIT expression patterns demonstrated a significant difference between KIT pattern 1 and KIT pattern 3, KIT pattern 3 showed a higher mean of eosinophil count. There was no significant correlation between eosinophil count and KIT patterns (p = 0.2648). However, a positive correlation was observed between the KIT patterns and Patnaik and Kiupel grades (p = 0.0006 and p = 0.0267, respectively). There was no significant correlation between eosinophil count, IL-5, or VEGFr. Further studies should determine whether eosinophil counts are an independent predictor of clinical outcome or simply correlated with already known predictors. |
| first_indexed | 2024-03-11T10:43:27Z |
| format | Article |
| id | doaj.art-009d5a3776174d5dbdaae5ec354de97d |
| institution | Directory Open Access Journal |
| issn | 1678-4162 |
| language | English |
| last_indexed | 2024-03-11T10:43:27Z |
| publishDate | 2023-11-01 |
| publisher | Universidade Federal de Minas Gerais |
| record_format | Article |
| series | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| spelling | doaj.art-009d5a3776174d5dbdaae5ec354de97d2023-11-14T07:50:19ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-41622023-11-017561047105410.1590/1678-4162-12963Comparison of KIT patterns and infiltration of eosinophils in canine mast cell tumorC.D. Araújohttps://orcid.org/0000-0002-8552-4078G.S. Sancheshttps://orcid.org/0000-0001-6264-8249F. Borekhttps://orcid.org/0000-0003-4532-0895D.C. Rochahttps://orcid.org/0000-0002-6912-3995G.D. Giustinahttps://orcid.org/0000-0003-4736-0591J.R. Engracia Filhohttps://orcid.org/0000-0002-2043-0652G.H. Becharahttps://orcid.org/0000-0003-4619-3744ABSTRACT KIT protein is associated with the etiology of canine mast cell tumors (MCT); however, the expression patterns of KIT are highly variable. The aim of this study was to determine if KIT patterns are related with eosinophil count in MCT. Hematoxylin eosin and May Grünwald-Giemsa stain techniques were applied, histological grading and eosinophil counting were performed in 48 MCT samples. Immunohistochemical evaluation was performed with IL-5, VEGFr, and c-KIT antibodies. The percentage of immunolabeling with IL-5 and VEGFr was determined, and the samples incubated with c-KIT were graded according to the immunolabeling pattern. Comparison of the mean eosinophil count between the histological grades and the different KIT expression patterns demonstrated a significant difference between KIT pattern 1 and KIT pattern 3, KIT pattern 3 showed a higher mean of eosinophil count. There was no significant correlation between eosinophil count and KIT patterns (p = 0.2648). However, a positive correlation was observed between the KIT patterns and Patnaik and Kiupel grades (p = 0.0006 and p = 0.0267, respectively). There was no significant correlation between eosinophil count, IL-5, or VEGFr. Further studies should determine whether eosinophil counts are an independent predictor of clinical outcome or simply correlated with already known predictors.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352023000601047&lng=en&tlng=endogsimmunohistochemistryeosinophilmast cellc-KIT |
| spellingShingle | C.D. Araújo G.S. Sanches F. Borek D.C. Rocha G.D. Giustina J.R. Engracia Filho G.H. Bechara Comparison of KIT patterns and infiltration of eosinophils in canine mast cell tumor Arquivo Brasileiro de Medicina Veterinária e Zootecnia dogs immunohistochemistry eosinophil mast cell c-KIT |
| title | Comparison of KIT patterns and infiltration of eosinophils in canine mast cell tumor |
| title_full | Comparison of KIT patterns and infiltration of eosinophils in canine mast cell tumor |
| title_fullStr | Comparison of KIT patterns and infiltration of eosinophils in canine mast cell tumor |
| title_full_unstemmed | Comparison of KIT patterns and infiltration of eosinophils in canine mast cell tumor |
| title_short | Comparison of KIT patterns and infiltration of eosinophils in canine mast cell tumor |
| title_sort | comparison of kit patterns and infiltration of eosinophils in canine mast cell tumor |
| topic | dogs immunohistochemistry eosinophil mast cell c-KIT |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352023000601047&lng=en&tlng=en |
| work_keys_str_mv | AT cdaraujo comparisonofkitpatternsandinfiltrationofeosinophilsincaninemastcelltumor AT gssanches comparisonofkitpatternsandinfiltrationofeosinophilsincaninemastcelltumor AT fborek comparisonofkitpatternsandinfiltrationofeosinophilsincaninemastcelltumor AT dcrocha comparisonofkitpatternsandinfiltrationofeosinophilsincaninemastcelltumor AT gdgiustina comparisonofkitpatternsandinfiltrationofeosinophilsincaninemastcelltumor AT jrengraciafilho comparisonofkitpatternsandinfiltrationofeosinophilsincaninemastcelltumor AT ghbechara comparisonofkitpatternsandinfiltrationofeosinophilsincaninemastcelltumor |