Aeromonas species isolated from aquatic organisms, insects, chicken, and humans in India show similar antimicrobial resistance profiles
Aeromonas species are Gram-negative bacteria that infect various living organisms and are ubiquitously found in different aquatic environments. In this study, we used whole genome sequencing (WGS) to identify and compare the antimicrobial resistance (AMR) genes, integrons, transposases and plasmids...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-12-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1008870/full |
| _version_ | 1828097626758709248 |
|---|---|
| author | Saurabh Dubey Eirill Ager-Wick Jitendra Kumar Indrani Karunasagar Iddya Karunasagar Bo Peng Øystein Evensen Henning Sørum Hetron M. Munang’andu Hetron M. Munang’andu |
| author_facet | Saurabh Dubey Eirill Ager-Wick Jitendra Kumar Indrani Karunasagar Iddya Karunasagar Bo Peng Øystein Evensen Henning Sørum Hetron M. Munang’andu Hetron M. Munang’andu |
| author_sort | Saurabh Dubey |
| collection | DOAJ |
| description | Aeromonas species are Gram-negative bacteria that infect various living organisms and are ubiquitously found in different aquatic environments. In this study, we used whole genome sequencing (WGS) to identify and compare the antimicrobial resistance (AMR) genes, integrons, transposases and plasmids found in Aeromonas hydrophila, Aeromonas caviae and Aeromonas veronii isolated from Indian major carp (Catla catla), Indian carp (Labeo rohita), catfish (Clarias batrachus) and Nile tilapia (Oreochromis niloticus) sampled in India. To gain a wider comparison, we included 11 whole genome sequences of Aeromonas spp. from different host species in India deposited in the National Center for Biotechnology Information (NCBI). Our findings show that all 15 Aeromonas sequences examined had multiple AMR genes of which the Ambler classes B, C and D β-lactamase genes were the most dominant. The high similarity of AMR genes in the Aeromonas sequences obtained from different host species point to interspecies transmission of AMR genes. Our findings also show that all Aeromonas sequences examined encoded several multidrug efflux-pump proteins. As for genes linked to mobile genetic elements (MBE), only the class I integrase was detected from two fish isolates, while all transposases detected belonged to the insertion sequence (IS) family. Only seven of the 15 Aeromonas sequences examined had plasmids and none of the plasmids encoded AMR genes. In summary, our findings show that Aeromonas spp. isolated from different host species in India carry multiple AMR genes. Thus, we advocate that the control of AMR caused by Aeromonas spp. in India should be based on a One Health approach. |
| first_indexed | 2024-04-11T07:50:03Z |
| format | Article |
| id | doaj.art-009d64a99c2c480c85362354d54a4061 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T07:50:03Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-009d64a99c2c480c85362354d54a40612022-12-22T04:36:07ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-12-011310.3389/fmicb.2022.10088701008870Aeromonas species isolated from aquatic organisms, insects, chicken, and humans in India show similar antimicrobial resistance profilesSaurabh Dubey0Eirill Ager-Wick1Jitendra Kumar2Indrani Karunasagar3Iddya Karunasagar4Bo Peng5Øystein Evensen6Henning Sørum7Hetron M. Munang’andu8Hetron M. Munang’andu9Section of Experimental Biomedicine, Department of Production Animal Clinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Ås, NorwaySection of Experimental Biomedicine, Department of Production Animal Clinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Ås, NorwayCollege of Fisheries, Acharya Narendra Deva University of Agriculture and Technology, Uttar Pradesh, IndiaNitte University Centre for Science Education and Research, Mangaluru, IndiaNitte University Centre for Science Education and Research, Mangaluru, IndiaState Key Laboratory of Biocontrol, Guangdong Key Laboratory of Pharmaceutical Functional Genes, School of Life Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Sun Yat-sen University, Higher Education Mega Center, Guangzhou, ChinaDepartment of Paraclinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Ås, NorwayDepartment of Paraclinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Ås, NorwaySection of Experimental Biomedicine, Department of Production Animal Clinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Ås, NorwayFaculty of Biosciences and Aquaculture, Nord University, Bodø, NorwayAeromonas species are Gram-negative bacteria that infect various living organisms and are ubiquitously found in different aquatic environments. In this study, we used whole genome sequencing (WGS) to identify and compare the antimicrobial resistance (AMR) genes, integrons, transposases and plasmids found in Aeromonas hydrophila, Aeromonas caviae and Aeromonas veronii isolated from Indian major carp (Catla catla), Indian carp (Labeo rohita), catfish (Clarias batrachus) and Nile tilapia (Oreochromis niloticus) sampled in India. To gain a wider comparison, we included 11 whole genome sequences of Aeromonas spp. from different host species in India deposited in the National Center for Biotechnology Information (NCBI). Our findings show that all 15 Aeromonas sequences examined had multiple AMR genes of which the Ambler classes B, C and D β-lactamase genes were the most dominant. The high similarity of AMR genes in the Aeromonas sequences obtained from different host species point to interspecies transmission of AMR genes. Our findings also show that all Aeromonas sequences examined encoded several multidrug efflux-pump proteins. As for genes linked to mobile genetic elements (MBE), only the class I integrase was detected from two fish isolates, while all transposases detected belonged to the insertion sequence (IS) family. Only seven of the 15 Aeromonas sequences examined had plasmids and none of the plasmids encoded AMR genes. In summary, our findings show that Aeromonas spp. isolated from different host species in India carry multiple AMR genes. Thus, we advocate that the control of AMR caused by Aeromonas spp. in India should be based on a One Health approach.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1008870/fullAeromonasresistanceplasmidsintegrasebeta lactamantimicrobials |
| spellingShingle | Saurabh Dubey Eirill Ager-Wick Jitendra Kumar Indrani Karunasagar Iddya Karunasagar Bo Peng Øystein Evensen Henning Sørum Hetron M. Munang’andu Hetron M. Munang’andu Aeromonas species isolated from aquatic organisms, insects, chicken, and humans in India show similar antimicrobial resistance profiles Frontiers in Microbiology Aeromonas resistance plasmids integrase beta lactam antimicrobials |
| title | Aeromonas species isolated from aquatic organisms, insects, chicken, and humans in India show similar antimicrobial resistance profiles |
| title_full | Aeromonas species isolated from aquatic organisms, insects, chicken, and humans in India show similar antimicrobial resistance profiles |
| title_fullStr | Aeromonas species isolated from aquatic organisms, insects, chicken, and humans in India show similar antimicrobial resistance profiles |
| title_full_unstemmed | Aeromonas species isolated from aquatic organisms, insects, chicken, and humans in India show similar antimicrobial resistance profiles |
| title_short | Aeromonas species isolated from aquatic organisms, insects, chicken, and humans in India show similar antimicrobial resistance profiles |
| title_sort | aeromonas species isolated from aquatic organisms insects chicken and humans in india show similar antimicrobial resistance profiles |
| topic | Aeromonas resistance plasmids integrase beta lactam antimicrobials |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1008870/full |
| work_keys_str_mv | AT saurabhdubey aeromonasspeciesisolatedfromaquaticorganismsinsectschickenandhumansinindiashowsimilarantimicrobialresistanceprofiles AT eirillagerwick aeromonasspeciesisolatedfromaquaticorganismsinsectschickenandhumansinindiashowsimilarantimicrobialresistanceprofiles AT jitendrakumar aeromonasspeciesisolatedfromaquaticorganismsinsectschickenandhumansinindiashowsimilarantimicrobialresistanceprofiles AT indranikarunasagar aeromonasspeciesisolatedfromaquaticorganismsinsectschickenandhumansinindiashowsimilarantimicrobialresistanceprofiles AT iddyakarunasagar aeromonasspeciesisolatedfromaquaticorganismsinsectschickenandhumansinindiashowsimilarantimicrobialresistanceprofiles AT bopeng aeromonasspeciesisolatedfromaquaticorganismsinsectschickenandhumansinindiashowsimilarantimicrobialresistanceprofiles AT øysteinevensen aeromonasspeciesisolatedfromaquaticorganismsinsectschickenandhumansinindiashowsimilarantimicrobialresistanceprofiles AT henningsørum aeromonasspeciesisolatedfromaquaticorganismsinsectschickenandhumansinindiashowsimilarantimicrobialresistanceprofiles AT hetronmmunangandu aeromonasspeciesisolatedfromaquaticorganismsinsectschickenandhumansinindiashowsimilarantimicrobialresistanceprofiles AT hetronmmunangandu aeromonasspeciesisolatedfromaquaticorganismsinsectschickenandhumansinindiashowsimilarantimicrobialresistanceprofiles |