Investigating quorum-quenching marine bacilli as potential biocontrol agents for protection of shrimps against Early Mortality Syndrome (EMS)
Abstract Early Mortality Syndrome (EMS) has been a major problem for shrimp aquaculture in Southeast Asia due to its epizootic prevalence within the region since the first reported case in 2009. This study explores the application of halophilic marine bacilli isolated from coral mucus and their quor...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-03-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-31197-4 |
| _version_ | 1797864873663660032 |
|---|---|
| author | Moritz Müller Andrew J. Spiers Angelica Tan Aazani Mujahid |
| author_facet | Moritz Müller Andrew J. Spiers Angelica Tan Aazani Mujahid |
| author_sort | Moritz Müller |
| collection | DOAJ |
| description | Abstract Early Mortality Syndrome (EMS) has been a major problem for shrimp aquaculture in Southeast Asia due to its epizootic prevalence within the region since the first reported case in 2009. This study explores the application of halophilic marine bacilli isolated from coral mucus and their quorum-quenching abilities as potential biocontrol agents in aquaculture systems to combat the causative agent of EMS, Vibrio parahaemolyticus. N-acylhomoserine lactone (AHL)-degrading (AiiA) activity was first screened by PCR then confirmed by bio-reporter assay, and a combination of 16S rDNA sequence analysis and quantitative phenotype assays including biofilm-formation and temperature-growth responses were used to demonstrate diversity amongst these quorum-quenching isolates. Three phenotypically distinct strains showing notable potential were chosen to undergo co-cultivation as a method for strain improvement via long term exposure to the pathogenic V. parahaemolyticus. The novel approach taken led to significant improvements in antagonism and quorum quenching activities as compared to the ancestral wild-type strains and offers a potential solution as well as pathway to improve existing beneficial microbes for one of the most pressing issues in shrimp aquacultures worldwide. |
| first_indexed | 2024-04-09T22:59:00Z |
| format | Article |
| id | doaj.art-00a0c180ff974876a3cfe52f80479a6b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T22:59:00Z |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-00a0c180ff974876a3cfe52f80479a6b2023-03-22T11:02:59ZengNature PortfolioScientific Reports2045-23222023-03-0113111010.1038/s41598-023-31197-4Investigating quorum-quenching marine bacilli as potential biocontrol agents for protection of shrimps against Early Mortality Syndrome (EMS)Moritz Müller0Andrew J. Spiers1Angelica Tan2Aazani Mujahid3Faculty of Engineering, Computing and Science, Swinburne University of Technology SarawakSchool of Science, Engineering and Technology, Abertay UniversityFaculty of Engineering, Computing and Science, Swinburne University of Technology SarawakInstitute of Biodiversity and Environmental Conservation (IBEC), Universiti Malaysia SarawakAbstract Early Mortality Syndrome (EMS) has been a major problem for shrimp aquaculture in Southeast Asia due to its epizootic prevalence within the region since the first reported case in 2009. This study explores the application of halophilic marine bacilli isolated from coral mucus and their quorum-quenching abilities as potential biocontrol agents in aquaculture systems to combat the causative agent of EMS, Vibrio parahaemolyticus. N-acylhomoserine lactone (AHL)-degrading (AiiA) activity was first screened by PCR then confirmed by bio-reporter assay, and a combination of 16S rDNA sequence analysis and quantitative phenotype assays including biofilm-formation and temperature-growth responses were used to demonstrate diversity amongst these quorum-quenching isolates. Three phenotypically distinct strains showing notable potential were chosen to undergo co-cultivation as a method for strain improvement via long term exposure to the pathogenic V. parahaemolyticus. The novel approach taken led to significant improvements in antagonism and quorum quenching activities as compared to the ancestral wild-type strains and offers a potential solution as well as pathway to improve existing beneficial microbes for one of the most pressing issues in shrimp aquacultures worldwide.https://doi.org/10.1038/s41598-023-31197-4 |
| spellingShingle | Moritz Müller Andrew J. Spiers Angelica Tan Aazani Mujahid Investigating quorum-quenching marine bacilli as potential biocontrol agents for protection of shrimps against Early Mortality Syndrome (EMS) Scientific Reports |
| title | Investigating quorum-quenching marine bacilli as potential biocontrol agents for protection of shrimps against Early Mortality Syndrome (EMS) |
| title_full | Investigating quorum-quenching marine bacilli as potential biocontrol agents for protection of shrimps against Early Mortality Syndrome (EMS) |
| title_fullStr | Investigating quorum-quenching marine bacilli as potential biocontrol agents for protection of shrimps against Early Mortality Syndrome (EMS) |
| title_full_unstemmed | Investigating quorum-quenching marine bacilli as potential biocontrol agents for protection of shrimps against Early Mortality Syndrome (EMS) |
| title_short | Investigating quorum-quenching marine bacilli as potential biocontrol agents for protection of shrimps against Early Mortality Syndrome (EMS) |
| title_sort | investigating quorum quenching marine bacilli as potential biocontrol agents for protection of shrimps against early mortality syndrome ems |
| url | https://doi.org/10.1038/s41598-023-31197-4 |
| work_keys_str_mv | AT moritzmuller investigatingquorumquenchingmarinebacilliaspotentialbiocontrolagentsforprotectionofshrimpsagainstearlymortalitysyndromeems AT andrewjspiers investigatingquorumquenchingmarinebacilliaspotentialbiocontrolagentsforprotectionofshrimpsagainstearlymortalitysyndromeems AT angelicatan investigatingquorumquenchingmarinebacilliaspotentialbiocontrolagentsforprotectionofshrimpsagainstearlymortalitysyndromeems AT aazanimujahid investigatingquorumquenchingmarinebacilliaspotentialbiocontrolagentsforprotectionofshrimpsagainstearlymortalitysyndromeems |