Cytotoxicity studies of Fe3O4 nanoparticles in chicken macrophage cells
Magnetic Fe3O4 nanoparticles (Fe3O4-NPs) have been widely investigated for their biomedical applications. The main purpose of this study was to evaluate the cytotoxic effects of different sizes of Fe3O4-NPs in chicken macrophage cells (HD11). Experimental groups based on three sizes of Fe3O4-NPs (60...
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| Format: | Article |
| Language: | English |
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The Royal Society
2020-04-01
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| Series: | Royal Society Open Science |
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| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.191561 |
| _version_ | 1819115618489597952 |
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| author | Shan Zhang Shu Wu Yiru Shen Yunqi Xiao Lizeng Gao Shourong Shi |
| author_facet | Shan Zhang Shu Wu Yiru Shen Yunqi Xiao Lizeng Gao Shourong Shi |
| author_sort | Shan Zhang |
| collection | DOAJ |
| description | Magnetic Fe3O4 nanoparticles (Fe3O4-NPs) have been widely investigated for their biomedical applications. The main purpose of this study was to evaluate the cytotoxic effects of different sizes of Fe3O4-NPs in chicken macrophage cells (HD11). Experimental groups based on three sizes of Fe3O4-NPs (60, 120 and 250 nm) were created, and the Fe3O4-NPs were added to the cells at different doses according to the experimental group. The cell activity, oxidative index (malondialdehyde (MDA), superoxide dismutase (SOD) and reactive oxygen species (ROS)), apoptosis and pro-inflammatory cytokine secretion level were detected to analyse the cytotoxic effects of Fe3O4-NPs of different sizes in HD11 cells. The results revealed that the cell viability of the 60 nm Fe3O4-NPs group was lower than those of the 120 and 250 nm groups when the same concentration of Fe3O4-NPs was added. No significant difference in MDA was observed among the three Fe3O4-NP groups. The SOD level and ROS production of the 60 nm group were significantly greater than those of the 120 and 250 nm groups. Furthermore, the highest levels of apoptosis and pro-inflammatory cytokine secretion were caused by the 60 nm Fe3O4-NPs. In conclusion, the smaller Fe3O4-NPs produced stronger cytotoxicity in chicken macrophage cells, and the cytotoxic effects may be related to the oxidative stress and apoptosis induced by increased ROS production as well as the increased expression of pro-inflammatory cytokines. |
| first_indexed | 2024-12-22T05:04:03Z |
| format | Article |
| id | doaj.art-00a16a30219b4a57bd56bfd1b611421e |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-12-22T05:04:03Z |
| publishDate | 2020-04-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-00a16a30219b4a57bd56bfd1b611421e2022-12-21T18:38:09ZengThe Royal SocietyRoyal Society Open Science2054-57032020-04-017410.1098/rsos.191561191561Cytotoxicity studies of Fe3O4 nanoparticles in chicken macrophage cellsShan ZhangShu WuYiru ShenYunqi XiaoLizeng GaoShourong ShiMagnetic Fe3O4 nanoparticles (Fe3O4-NPs) have been widely investigated for their biomedical applications. The main purpose of this study was to evaluate the cytotoxic effects of different sizes of Fe3O4-NPs in chicken macrophage cells (HD11). Experimental groups based on three sizes of Fe3O4-NPs (60, 120 and 250 nm) were created, and the Fe3O4-NPs were added to the cells at different doses according to the experimental group. The cell activity, oxidative index (malondialdehyde (MDA), superoxide dismutase (SOD) and reactive oxygen species (ROS)), apoptosis and pro-inflammatory cytokine secretion level were detected to analyse the cytotoxic effects of Fe3O4-NPs of different sizes in HD11 cells. The results revealed that the cell viability of the 60 nm Fe3O4-NPs group was lower than those of the 120 and 250 nm groups when the same concentration of Fe3O4-NPs was added. No significant difference in MDA was observed among the three Fe3O4-NP groups. The SOD level and ROS production of the 60 nm group were significantly greater than those of the 120 and 250 nm groups. Furthermore, the highest levels of apoptosis and pro-inflammatory cytokine secretion were caused by the 60 nm Fe3O4-NPs. In conclusion, the smaller Fe3O4-NPs produced stronger cytotoxicity in chicken macrophage cells, and the cytotoxic effects may be related to the oxidative stress and apoptosis induced by increased ROS production as well as the increased expression of pro-inflammatory cytokines.https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.191561magnetic fe3o4 nanoparticlescytotoxic effectsoxidative indexapoptosischicken macrophage cells |
| spellingShingle | Shan Zhang Shu Wu Yiru Shen Yunqi Xiao Lizeng Gao Shourong Shi Cytotoxicity studies of Fe3O4 nanoparticles in chicken macrophage cells Royal Society Open Science magnetic fe3o4 nanoparticles cytotoxic effects oxidative index apoptosis chicken macrophage cells |
| title | Cytotoxicity studies of Fe3O4 nanoparticles in chicken macrophage cells |
| title_full | Cytotoxicity studies of Fe3O4 nanoparticles in chicken macrophage cells |
| title_fullStr | Cytotoxicity studies of Fe3O4 nanoparticles in chicken macrophage cells |
| title_full_unstemmed | Cytotoxicity studies of Fe3O4 nanoparticles in chicken macrophage cells |
| title_short | Cytotoxicity studies of Fe3O4 nanoparticles in chicken macrophage cells |
| title_sort | cytotoxicity studies of fe3o4 nanoparticles in chicken macrophage cells |
| topic | magnetic fe3o4 nanoparticles cytotoxic effects oxidative index apoptosis chicken macrophage cells |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.191561 |
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