LCRMP-1 is required for spermatogenesis and stabilises spermatid F-actin organization via the PI3K-Akt pathway
Abstract Long-form collapsin response mediator protein-1 (LCRMP-1) belongs to the CRMP family which comprises brain-enriched proteins responsible for axon guidance. However, its role in spermatogenesis remains unclear. Here we find that LCRMP-1 is abundantly expressed in the testis. To characterize...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-04-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-023-04778-2 |
| _version_ | 1797845783731503104 |
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| author | Jung-Hsuan Chang Chia-Hua Chou Jui-Ching Wu Keng-Mao Liao Wei-Jia Luo Wei-Lun Hsu Xuan-Ren Chen Sung-Liang Yu Szu-Hua Pan Pan-Chyr Yang Kang-Yi Su |
| author_facet | Jung-Hsuan Chang Chia-Hua Chou Jui-Ching Wu Keng-Mao Liao Wei-Jia Luo Wei-Lun Hsu Xuan-Ren Chen Sung-Liang Yu Szu-Hua Pan Pan-Chyr Yang Kang-Yi Su |
| author_sort | Jung-Hsuan Chang |
| collection | DOAJ |
| description | Abstract Long-form collapsin response mediator protein-1 (LCRMP-1) belongs to the CRMP family which comprises brain-enriched proteins responsible for axon guidance. However, its role in spermatogenesis remains unclear. Here we find that LCRMP-1 is abundantly expressed in the testis. To characterize its physiological function, we generate LCRMP-1-deficient mice (Lcrmp-1 −/− ). These mice exhibit aberrant spermiation with apoptotic spermatids, oligospermia, and accumulation of immature testicular cells, contributing to reduced fertility. In the seminiferous epithelial cycle, LCRMP-1 expression pattern varies in a stage-dependent manner. LCRMP-1 is highly expressed in spermatids during spermatogenesis and especially localized to the spermiation machinery during spermiation. Mechanistically, LCRMP-1 deficiency causes disorganized F-actin due to unbalanced signaling of F-actin dynamics through upregulated PI3K-Akt-mTOR signaling. In conclusion, LCRMP-1 maintains spermatogenesis homeostasis by modulating cytoskeleton remodeling for spermatozoa release. |
| first_indexed | 2024-04-09T17:44:32Z |
| format | Article |
| id | doaj.art-00a2c279cbcf45a98307696afbe0f2ab |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-04-09T17:44:32Z |
| publishDate | 2023-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-00a2c279cbcf45a98307696afbe0f2ab2023-04-16T11:22:28ZengNature PortfolioCommunications Biology2399-36422023-04-016111510.1038/s42003-023-04778-2LCRMP-1 is required for spermatogenesis and stabilises spermatid F-actin organization via the PI3K-Akt pathwayJung-Hsuan Chang0Chia-Hua Chou1Jui-Ching Wu2Keng-Mao Liao3Wei-Jia Luo4Wei-Lun Hsu5Xuan-Ren Chen6Sung-Liang Yu7Szu-Hua Pan8Pan-Chyr Yang9Kang-Yi Su10Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan UniversityDepartment of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan UniversityDepartment of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan UniversityGenome and Systems Biology Degree Program, National Taiwan University and Academia SinicaDepartment of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan UniversityDepartment of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan UniversityGraduate Institute of Medical Genomics and Proteomics, College of Medicine, National Taiwan UniversityDepartment of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan UniversityGenome and Systems Biology Degree Program, National Taiwan University and Academia SinicaDepartment of Internal Medicine, College of Medicine, National Taiwan UniversityDepartment of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan UniversityAbstract Long-form collapsin response mediator protein-1 (LCRMP-1) belongs to the CRMP family which comprises brain-enriched proteins responsible for axon guidance. However, its role in spermatogenesis remains unclear. Here we find that LCRMP-1 is abundantly expressed in the testis. To characterize its physiological function, we generate LCRMP-1-deficient mice (Lcrmp-1 −/− ). These mice exhibit aberrant spermiation with apoptotic spermatids, oligospermia, and accumulation of immature testicular cells, contributing to reduced fertility. In the seminiferous epithelial cycle, LCRMP-1 expression pattern varies in a stage-dependent manner. LCRMP-1 is highly expressed in spermatids during spermatogenesis and especially localized to the spermiation machinery during spermiation. Mechanistically, LCRMP-1 deficiency causes disorganized F-actin due to unbalanced signaling of F-actin dynamics through upregulated PI3K-Akt-mTOR signaling. In conclusion, LCRMP-1 maintains spermatogenesis homeostasis by modulating cytoskeleton remodeling for spermatozoa release.https://doi.org/10.1038/s42003-023-04778-2 |
| spellingShingle | Jung-Hsuan Chang Chia-Hua Chou Jui-Ching Wu Keng-Mao Liao Wei-Jia Luo Wei-Lun Hsu Xuan-Ren Chen Sung-Liang Yu Szu-Hua Pan Pan-Chyr Yang Kang-Yi Su LCRMP-1 is required for spermatogenesis and stabilises spermatid F-actin organization via the PI3K-Akt pathway Communications Biology |
| title | LCRMP-1 is required for spermatogenesis and stabilises spermatid F-actin organization via the PI3K-Akt pathway |
| title_full | LCRMP-1 is required for spermatogenesis and stabilises spermatid F-actin organization via the PI3K-Akt pathway |
| title_fullStr | LCRMP-1 is required for spermatogenesis and stabilises spermatid F-actin organization via the PI3K-Akt pathway |
| title_full_unstemmed | LCRMP-1 is required for spermatogenesis and stabilises spermatid F-actin organization via the PI3K-Akt pathway |
| title_short | LCRMP-1 is required for spermatogenesis and stabilises spermatid F-actin organization via the PI3K-Akt pathway |
| title_sort | lcrmp 1 is required for spermatogenesis and stabilises spermatid f actin organization via the pi3k akt pathway |
| url | https://doi.org/10.1038/s42003-023-04778-2 |
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