Ex-situ bioremediation of U(VI) from contaminated mine water using Acidithiobacillus ferrooxidans strains
The ex-situ bioremoval of U(VI) from contaminated water using Acidithiobacillus ferrooxidans strain 8455 and 13538 was studied under a range of pH and uranium concentrations. The effect of pH on the growth of bacteria was evaluated across the range 1.5 – 4.5 pH units. The respiration rate of At. fer...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-05-01
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| Series: | Frontiers in Environmental Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fenvs.2016.00039/full |
| _version_ | 1811202744061526016 |
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| author | Maria eRomero-Gonzalez Bennett eNwaobi Joseph Michael Hufton Daniel James Gilmour |
| author_facet | Maria eRomero-Gonzalez Bennett eNwaobi Joseph Michael Hufton Daniel James Gilmour |
| author_sort | Maria eRomero-Gonzalez |
| collection | DOAJ |
| description | The ex-situ bioremoval of U(VI) from contaminated water using Acidithiobacillus ferrooxidans strain 8455 and 13538 was studied under a range of pH and uranium concentrations. The effect of pH on the growth of bacteria was evaluated across the range 1.5 – 4.5 pH units. The respiration rate of At. ferrooxidans at different U(VI) concentrations was quantified as a measure of the rate of metabolic activity over time using an oxygen electrode. The biosorption process was quantified using a uranyl nitrate solution, U-spiked growth media and U-contaminated mine water. The results showed that both strains of At. ferrooxidans are able to remove U(VI) from solution at pH 2.5 – 4.5, exhibiting a buffering capacity at pH 3.5. The respiration rate of the micro-organism was affected at U(VI) concentration of 30 mg L-1. The kinetics of the sorption fitted a pseudo-first order equation, and depended on the concentration of U(VI). The KD obtained from the biosorption experiments indicated that strain 8455 is more efficient for the removal of U(VI). A bioreactor designed to treat a solution of 100 mg U(VI) L-1 removed at least 50% of the U(VI) in water. The study demonstrated that At. ferrooxidans can be used for the ex-situ bioremediation of U(VI) contaminated mine water. |
| first_indexed | 2024-04-12T02:44:54Z |
| format | Article |
| id | doaj.art-00a4002c688f4d199187355b7e42020f |
| institution | Directory Open Access Journal |
| issn | 2296-665X |
| language | English |
| last_indexed | 2024-04-12T02:44:54Z |
| publishDate | 2016-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Environmental Science |
| spelling | doaj.art-00a4002c688f4d199187355b7e42020f2022-12-22T03:51:13ZengFrontiers Media S.A.Frontiers in Environmental Science2296-665X2016-05-01410.3389/fenvs.2016.00039187156Ex-situ bioremediation of U(VI) from contaminated mine water using Acidithiobacillus ferrooxidans strainsMaria eRomero-Gonzalez0Bennett eNwaobi1Joseph Michael Hufton2Daniel James Gilmour3The University of SheffieldImperial Colleague LondonThe University of SheffieldThe University of SheffieldThe ex-situ bioremoval of U(VI) from contaminated water using Acidithiobacillus ferrooxidans strain 8455 and 13538 was studied under a range of pH and uranium concentrations. The effect of pH on the growth of bacteria was evaluated across the range 1.5 – 4.5 pH units. The respiration rate of At. ferrooxidans at different U(VI) concentrations was quantified as a measure of the rate of metabolic activity over time using an oxygen electrode. The biosorption process was quantified using a uranyl nitrate solution, U-spiked growth media and U-contaminated mine water. The results showed that both strains of At. ferrooxidans are able to remove U(VI) from solution at pH 2.5 – 4.5, exhibiting a buffering capacity at pH 3.5. The respiration rate of the micro-organism was affected at U(VI) concentration of 30 mg L-1. The kinetics of the sorption fitted a pseudo-first order equation, and depended on the concentration of U(VI). The KD obtained from the biosorption experiments indicated that strain 8455 is more efficient for the removal of U(VI). A bioreactor designed to treat a solution of 100 mg U(VI) L-1 removed at least 50% of the U(VI) in water. The study demonstrated that At. ferrooxidans can be used for the ex-situ bioremediation of U(VI) contaminated mine water.http://journal.frontiersin.org/Journal/10.3389/fenvs.2016.00039/fullbioreactorMine waterAt. ferrooxidansEx-situ processbioremediation of uranium |
| spellingShingle | Maria eRomero-Gonzalez Bennett eNwaobi Joseph Michael Hufton Daniel James Gilmour Ex-situ bioremediation of U(VI) from contaminated mine water using Acidithiobacillus ferrooxidans strains Frontiers in Environmental Science bioreactor Mine water At. ferrooxidans Ex-situ process bioremediation of uranium |
| title | Ex-situ bioremediation of U(VI) from contaminated mine water using Acidithiobacillus ferrooxidans strains |
| title_full | Ex-situ bioremediation of U(VI) from contaminated mine water using Acidithiobacillus ferrooxidans strains |
| title_fullStr | Ex-situ bioremediation of U(VI) from contaminated mine water using Acidithiobacillus ferrooxidans strains |
| title_full_unstemmed | Ex-situ bioremediation of U(VI) from contaminated mine water using Acidithiobacillus ferrooxidans strains |
| title_short | Ex-situ bioremediation of U(VI) from contaminated mine water using Acidithiobacillus ferrooxidans strains |
| title_sort | ex situ bioremediation of u vi from contaminated mine water using acidithiobacillus ferrooxidans strains |
| topic | bioreactor Mine water At. ferrooxidans Ex-situ process bioremediation of uranium |
| url | http://journal.frontiersin.org/Journal/10.3389/fenvs.2016.00039/full |
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