Bacillus subtilis spores displaying RBD domain of SARS-CoV-2 spike protein
Bacillus subtilis spores are considered to be efficient and useful vehicles for the surface display and delivery of heterologous proteins. In this study, we prepared recombinant spores with the receptor binding domain (RBD) of the SARS-CoV-2 spike glycoprotein displayed on their surface in fusion wi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Computational and Structural Biotechnology Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2001037023000557 |
| _version_ | 1797384109381648384 |
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| author | A. Vetráková R. Kalianková Chovanová R. Rechtoríková D. Krajčíková I. Barák |
| author_facet | A. Vetráková R. Kalianková Chovanová R. Rechtoríková D. Krajčíková I. Barák |
| author_sort | A. Vetráková |
| collection | DOAJ |
| description | Bacillus subtilis spores are considered to be efficient and useful vehicles for the surface display and delivery of heterologous proteins. In this study, we prepared recombinant spores with the receptor binding domain (RBD) of the SARS-CoV-2 spike glycoprotein displayed on their surface in fusion with the CotZ or CotY spore coat proteins as a possible tool for the development of an oral vaccine against the SARS-CoV-2 virus. The RBD was attached to the N-terminus or C-terminus of the coat proteins. We also directly adsorbed non-recombinantly produced RBD to the spore surface. SDS-PAGE, western blot and fluorescence microscopy were used to analyze RBD surface expression on purified spores. Results obtained from both display systems, recombinant and non-recombinant, demonstrated that RBD was present on the spore surfaces. |
| first_indexed | 2024-03-08T21:30:45Z |
| format | Article |
| id | doaj.art-00a9a354fe2246429f4be68a84ad2415 |
| institution | Directory Open Access Journal |
| issn | 2001-0370 |
| language | English |
| last_indexed | 2024-03-08T21:30:45Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Computational and Structural Biotechnology Journal |
| spelling | doaj.art-00a9a354fe2246429f4be68a84ad24152023-12-21T07:30:59ZengElsevierComputational and Structural Biotechnology Journal2001-03702023-01-012115501556Bacillus subtilis spores displaying RBD domain of SARS-CoV-2 spike proteinA. Vetráková0R. Kalianková Chovanová1R. Rechtoríková2D. Krajčíková3I. Barák4Department of Microbial Genetics, Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, SlovakiaDepartment of Microbial Genetics, Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, SlovakiaDepartment of Microbial Genetics, Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, SlovakiaDepartment of Microbial Genetics, Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, SlovakiaCorresponding author.; Department of Microbial Genetics, Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, SlovakiaBacillus subtilis spores are considered to be efficient and useful vehicles for the surface display and delivery of heterologous proteins. In this study, we prepared recombinant spores with the receptor binding domain (RBD) of the SARS-CoV-2 spike glycoprotein displayed on their surface in fusion with the CotZ or CotY spore coat proteins as a possible tool for the development of an oral vaccine against the SARS-CoV-2 virus. The RBD was attached to the N-terminus or C-terminus of the coat proteins. We also directly adsorbed non-recombinantly produced RBD to the spore surface. SDS-PAGE, western blot and fluorescence microscopy were used to analyze RBD surface expression on purified spores. Results obtained from both display systems, recombinant and non-recombinant, demonstrated that RBD was present on the spore surfaces.http://www.sciencedirect.com/science/article/pii/S2001037023000557Bacillus subtilisSpore surface displayCotZCotYRBD |
| spellingShingle | A. Vetráková R. Kalianková Chovanová R. Rechtoríková D. Krajčíková I. Barák Bacillus subtilis spores displaying RBD domain of SARS-CoV-2 spike protein Computational and Structural Biotechnology Journal Bacillus subtilis Spore surface display CotZ CotY RBD |
| title | Bacillus subtilis spores displaying RBD domain of SARS-CoV-2 spike protein |
| title_full | Bacillus subtilis spores displaying RBD domain of SARS-CoV-2 spike protein |
| title_fullStr | Bacillus subtilis spores displaying RBD domain of SARS-CoV-2 spike protein |
| title_full_unstemmed | Bacillus subtilis spores displaying RBD domain of SARS-CoV-2 spike protein |
| title_short | Bacillus subtilis spores displaying RBD domain of SARS-CoV-2 spike protein |
| title_sort | bacillus subtilis spores displaying rbd domain of sars cov 2 spike protein |
| topic | Bacillus subtilis Spore surface display CotZ CotY RBD |
| url | http://www.sciencedirect.com/science/article/pii/S2001037023000557 |
| work_keys_str_mv | AT avetrakova bacillussubtilissporesdisplayingrbddomainofsarscov2spikeprotein AT rkaliankovachovanova bacillussubtilissporesdisplayingrbddomainofsarscov2spikeprotein AT rrechtorikova bacillussubtilissporesdisplayingrbddomainofsarscov2spikeprotein AT dkrajcikova bacillussubtilissporesdisplayingrbddomainofsarscov2spikeprotein AT ibarak bacillussubtilissporesdisplayingrbddomainofsarscov2spikeprotein |